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11.
N. G. Katzen 《BMJ (Clinical research ed.)》1964,1(5386):839-840
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A novel method for generation of insect-based cell-free translation extracts is presented. The protocol can be completed in less than an hour, and the resulting extracts are extremely proficient in N-linked glycosylation and signal sequence processing. No specialized equipment other than that usually present in an ordinary biochemistry laboratory is required. The novel approach dramatically reduces cost and time while rendering enhanced lysates compared to previously published strategies. 相似文献
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New Mobilizable Vectors Suitable for Gene Replacement in Gram-Negative Bacteria and Their Use in Mapping of the 3′ End of the Xanthomonas campestris pv. campestris gum Operon 下载免费PDF全文
Federico Katzen Anke Becker M. Vernica Ielmini Cristian G. Oddo Luis Ielpi 《Applied microbiology》1999,65(1):278-282
We describe useful vectors to select double-crossover events directly in site-directed marker exchange mutagenesis in gram-negative bacteria. These vectors contain the gusA marker gene, providing colorimetric screens to identify bacteria harboring those sequences. The applicability of these vectors was shown by mapping the 3′ end of the Xanthomonas campestris gum operon, involved in biosynthesis of xanthan. 相似文献
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Endoscopic approach for the resection of forehead masses 总被引:3,自引:0,他引:3
Cronin ED Ruiz-Razura A Livingston CK Katzen JT 《Plastic and reconstructive surgery》2000,105(7):2459-2463
Over the past several years, surgery aided by the endoscope has come into favor for a number of reasons. Because it is minimally invasive surgery, it has less morbidity, thus, reduced postoperative pain and complications. It results in earlier mobilization and shorter hospitalization, and most importantly, it contributes to an improved cosmetic appearance as a result of a shortened incision line concealed within the hairline in most cases. We have proposed an alternative approach to the surgical resection of forehead masses by means of the endoscope, which has proven to be useful not only for diagnosis but also as a therapeutic tool for the removal of forehead lesions. This report described the clinical experience with the removal of forehead masses in four patients. The cases illustrated the feasibility and ease of resecting a variety of forehead masses with excellent cosmetic results. We hope that more plastic surgeons will use the proposed technique and will continue to explore the safe limits of endoscopic plastic surgery. 相似文献
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Xanthan gum is a complex exopolysaccharide produced by the plant-pathogenic bacterium Xanthomonas campestris pv. campestris. It consists of D-glucosyl, D-mannosyl, and D-glucuronyl acid residues in a molar ratio of 2:2:1 and variable proportions of O-acetyl and pyruvyl residues. Because of its physical properties, it is widely used as a thickener or viscosifier in both
food and non-food industries. Xanthan gum is also used as a stabilizer for a wide variety of suspensions, emulsions, and foams.
This article outlines aspects of the biochemical assembly and genetic loci involved in its biosynthesis, including the synthesis
of the sugar nucleotide substrates, the building and decoration of the pentasaccharide subunit, and the polymerization and
secretion of the polymer. An overview of the applications and industrial production of xanthan is also covered.
Received: 18 March 1998 / Received revision: 29 April 1998 / Accepted: 30 April 1998 相似文献
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Frederick SB Kibenge Marcos G Godoy Yingwei Wang Molly JT Kibenge Valentina Gherardelli Soledad Mansilla Angelica Lisperger Miguel Jarpa Geraldine Larroquete Fernando Avendaño Marcela Lara Alicia Gallardo 《Virology journal》2009,6(1):1-16