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551.
Katsuyuki Terada 《Mycoscience》2001,42(1):1-9
Laboulbenia stenolophi is reported for the first time from Japan.Stenolophus iridicolor andS. propinquus are added as new hosts. The crowded antheridia and the protruding (bulging) cell IV are characteristic of this fungus, although
thalli with an almost normal cell IV sometimes occur.Laboulbenia anoplogenii onAnoplogenius is easily distinguished from what has been calledL. anoplogenii onStenolophus, Astigis, Abacetus andChlaeminus by the following characters: 1) no crowded antheridia can be observed throughout thallus development, but many sterile long
branches can be observed instead; 2) cell IV starts to undergo cell division early in thallus development; 3) cell V extends
downward to the level of cell VII, rather than extending only as far as the perithecial basal cells; and 4) cell VI is usually
longer and extends farther distally than cell III.Anoplogenius is the host genus ofL. anoplogenii and the records from other hosts represent misapplied names. 相似文献
552.
GLUT4 translocation is not impaired after acute exercise in skeletal muscle of women with obesity and polycystic ovary syndrome
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Wagner Silva Dantas José Antonio Miguel Marcondes Samuel Katsuyuki Shinjo Luiz Augusto Perandini Vanessa Olzon Zambelli Willian Das Neves Cristiano Roberto Grimaldi Barcellos Michele Patrocínio Rocha Viviane Dos Reis Vieira Yance Renato Tavares Dos Santos Pereira Igor Hisashi Murai Ana Lucia De Sá Pinto Hamilton Roschel Bruno Gualano 《Obesity (Silver Spring, Md.)》2015,23(11):2207-2215
553.
A new method for the isolation of a high yield of collagen from human skeletal muscle is described. This technique employs rapid stirring of the homogenate of skeletal muscle with a magnetic stirrer. Fibrous material entangled during rapid stirring was recovered by passing the homogenate through a sieve and then sequentially extracted with Hasselbach-Schneider solution and 0.6 m KI-0.06 m Na2S2O3. The insoluble residue obtained after these extractions was shown to be highly purified collagen by amino acid analysis, and the recovery of collagen by this method was found to be more than 90% of the total collagen in skeletal muscle. The isolated collagen from 5 years of age was mostly composed of Type I collagen, and small amounts of Type III collagen and an unidentified collagenous protein migrating in a position near Type V collagen (αB chain) were also found on a sodium dodecyl sulfate-polyacrylamide gel. 相似文献
554.
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556.
Naosuke Kojima M. Sato Katsuyuki Imai Mitsutaka Miura Yoshikazu Matano Haruki Senoo 《Histochemistry and cell biology》1998,110(2):121-128
When cultured on a polystyrene surface or aminoalkylsilane-coated cover glasses, rat and human hepatic stellate cells exhibit
a flattened, fibroblast-like shape with well-developed stress fibers. However, culturing the cells on type I collagen gel
results in the elongation of long, multipolar cellular processes, whereas cells cultured on Matrigel maintain their round
shapes. Dual fluorescence staining of microtubules and fibrillar actin indicated that the processes extend together with collagen
fibers and contained microtubules as the core, whereas the periphery contained fibrillar actin. Immunofluorescence staining
of vinculin showed that the focal adhesions were distributed mainly in lamellipodia when cultured on aminoalkylsilane-coated
cover glasses, whereas in the cells cultured on type I collagen gel they were localized to the tips of the processes and along
their bottom surface contacting collagen fibers. Wortmannin, as well as staurosporin and herbimycin A, inhibited the elongation
process and induced the retraction of elongated processes. The wortmannin treatment also resulted in an alteration in focal
adhesion distribution from the processes to cell bodies. These results indicate that the cell surface integrin binding to
interstitial collagen fibers induces the elongation of processes through signaling events and the subsequent cytoskeleton
assembly in hepatic stellate cells.
Accepted: 12 February 1998 相似文献