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981.
982.
Summary An Escherichia coli mutant harbouring altered ribosomal protein L32 has been isolated and genetically characterized. The mutation leading to this alteration (rpmF) and the temperature-sensitive mutation (ts-1517) present in the same strain were found to map near pyrC (23.4 min), being cotransducible not only with pyrC but also with fabD, flaT and purB in P1 phage mediated transductions. Furthermore, we found that the gene rimJ, which encodes an enzyme that acetylates the N-terminal alanine of protein S5 and the temperature-sensitive mutation, ts-386, present in the rimJ mutant strain (Cumberlidge and Isono 1979) also mapped in this region. Thus, the order of genes is deduced to be: ts-386-pyrC-ts-1517-rimJ-flaT-fabD-rpmF-purB. 相似文献
983.
984.
Summary Temperature-sensitive mutants of an Escherichia coli K-12 strain PA3092 have been isolated following mutagenesis with nitrosoguanidine, and their ribosomal proteins analyzed by two-dimensional gel electrophoresis This method was found to be very efficient in obtaining mutants with various structural alterations in ribosomal proteins. Thus a total of some 160 mutants with alterations in 41 different ribosomal proteins have so far been isolated. By characterizing these mutants, we could isolate, not only those mutants with alterations in the structural genes for various ribosomal proteins, but also those with impairments in the modification of proteins S5, S18 and L12. Furthermore, a mutant has been obtained which apparently lacks the protein S20 (L26) with a concomitant reduction to a great extent of the polypeptide synthetic activity of the small subunit. The usefulness of these mutants in establishing the genetic architecture of the genes coding for the ribosomal proteins and their modifiers is discussed. 相似文献
985.
Keiko Nishizawa Ayumi Oguma Maki Kawata Yuji Sakasegawa Kenta Teruya Katsumi Doh-ura 《Journal of virology》2014,88(8):4083-4099
986.
Cultured green Lavandula vera cells were irradiated with variousdosages of gamma rays which increased the variation in the amountof free biotin produced by the cell clones. Variant sublinescontaining much more free biotin than the original line wereobtained by repeated selection. The effectiveness of gamma raysfor the induction of the variant sublines is described. (Received June 2, 1982; Accepted September 14, 1982) 相似文献
987.
988.
Yasushi Itoh Kumi Akataki Katsumi Mita Makoto Watakabe Hisako Nonaka 《Journal of electromyography and kinesiology》2013,23(3):572-579
The aims of the present study were to develop a mathematical model of the skeletal muscle based on the frequency transfer function, referred to as frequency response model, and to presume the relationship between the model elements and skeletal muscle contractile properties. Twitch force in elbow flexion was elicited by applying a single electrical stimulation to the motor point of biceps brachii muscles, and then analyzed visually by the Bode gain and phase diagram of the force signal. The frequency response model was represented by a frequency transfer function consisting of five basic control elements (proportional element, dead time element, and three first-order lag elements). The model element constants were estimated by best-fitting to the Bode gain and phase diagram of the twitch force signal. The proportional constant and the dead time in the frequency response model correlated significantly with the peak torque and the latency in the actual twitch force, respectively. In addition, the time constants of the three first-order lag elements in the model correlated strongly with the contraction time and the half relaxation time in the actual twitch force. The results suggested a possibility that the individual elements in the frequency response model would reflect the biochemical and biomechanical properties in the excitation–contraction coupling process of skeletal muscle. 相似文献
989.
Extracts of a coloured malt contained 4-hydroxy-5-monomethyl-3(2H)-furanone (HMMF), 4-hydroxy-2,5-dimethyl-3(2H)-furanone
(HDMF) but not 4-hydroxy-5(or 2)-ethyl-2(or 5)-methyl-3(2H)-furanone (HEMF). Extracts of a pale malt did not contain any of
the furanones. HMMF and HDMF were produced by Saccharomyces cerevisiae during fermen-tation of both types of malt extract.
About 0.09 mg HEMF l −1 was synthesised during fermentation of the coloured malt extract but none was produced with the pale malt extract. Final
concentrations of HDMF (2.0 mgl −1) and HEMF (0.09 mgl −1) were in excess of their aroma threshold values in water (0.16 and 0.02 mgl −1 respectively) after fermen-tation of the coloured malt extract.
This revised version was published online in November 2006 with corrections to the Cover Date. 相似文献
990.
Minamino T Kinoshita M Hara N Takeuchi S Hida A Koya S Glenwright H Imada K Aldridge PD Namba K 《Molecular microbiology》2012,83(4):775-788
FlgN chaperone acts as a bodyguard to protect its cognate substrates, FlgK and FlgL, from proteolysis in the cytoplasm. Docking of the FlgN-FlgK complex with the FliI ATPase of the flagellar type III export apparatus is key to the protein export process. However, a ΔfliH-fliI flhB(P28T) mutant forms some flagella even in the absence of FliH and FliI, raising the question of how FlgN promotes the export of its cognate substrates. Here, we report that the interaction of FlgN with an integral membrane export protein, FlhA, is directly involved in efficient protein export. A ΔfliH-fliI flhB(P28T) ΔflgN mutant caused extragenic suppressor mutations in the C-terminal domain of FlhA (FlhA(C) ). Pull-down assays using GST affinity chromatography showed an interaction between FlgN and FlhA(C) . The FlgN-FlgK complex bound to FlhA(C) and FliJ to form the FlgN-FlgK-FliJ-FlhA(C) complex. The FlgN-FlhA(C) interaction was enhanced by FlgK but not by FliJ. FlgN120 missing the last 20 residues still bound to FlgK and FliJ but not to FlhA(C) . A highly conserved Tyr-122 residue was required for the interaction with FlhA(C) . These results suggest that FlgN efficiently transfers FlgK/L subunits to FlhA(C) to promote their export. 相似文献