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81.
Moree WJ Kataoka K Ramirez-Weinhouse MM Shiota T Imai M Tsutsumi T Sudo M Endo N Muroga Y Hada T Fanning D Saunders J Kato Y Myers PL Tarby CM 《Bioorganic & medicinal chemistry letters》2008,18(6):1869-1873
SAR studies were conducted around lead compound 1 using high-throughput parallel solution and solid phase synthesis. Our lead optimization efforts led to the identification of several CCR2b antagonists with potent activity in both binding and functional assays [Compound 71 CCR2b Binding IC(50) 3.2 nM; MCP-1-Induced Chemotaxis IC(50) 0.83 nM; Ca(2+) Flux IC(50) 7.5 nM]. 相似文献
82.
Hidenori Ito Kimie Atsuzawa† Rika Morishita Nobuteru Usuda† Kaori Sudo Ikuko Iwamoto Kosuke Mizutani‡ Ritsuko Katoh-Semba Yoshinori Nozawa‡ Tomiko Asano Koh-ichi Nagata 《Journal of neurochemistry》2009,108(4):867-880
Septins, a conserved family of GTP/GDP-binding proteins, are present in organisms as diverse as yeast and mammals. We analyzed the distribution of five septins, Sept6, Sept7, Sept8, Sept9 and Sept11, in various rat tissues by western blot analyses and found all septins to be expressed in brain. We also examined the developmental changes of expression of these septins in the rat brain and found that the level of Sept8 increased during post-natal development. Morphological analyses revealed that Sept8 is enriched at pre-synapses. Using yeast two-hybrid screening, we identified vesicle-associated membrane protein 2 (VAMP2), a soluble N -ethylmaleimide-sensitive factor attachment protein receptor (SNARE), as an interacting protein for Sept8. Synaptophysin is reported to associate with and recruit VAMP2 to synaptic vesicles and dissociate prior to forming the SNARE complex consisting of VAMP2, syntaxin and synaptosome-associated protein of 25 kDa. We showed that Sept8 suppresses the interaction between VAMP2 and synaptophysin through binding to VAMP2. In addition, we found that Sept8 forms a complex with syntaxin1A, and the Sept8-VAMP2 interaction is disrupted by synaptosome-associated protein of 25 kDa. These results suggest that Sept8 may participate in the process of the SNARE complex formation and subsequent neurotransmitter release. 相似文献
83.
84.
Daisuke Suzuki Keiichi Inoue Makoto Sakai Hideki Kandori Yuki Sudo 《Journal of molecular biology》2009,392(1):48-4922
Microbial organisms utilize light not only as energy sources but also as signals by which rhodopsins (containing retinal as a chromophore) work as photoreceptors. Sensory rhodopsin I (SRI) is a dual photoreceptor that regulates both negative and positive phototaxis in microbial organisms, such as the archaeon Halobacterium salinarum and the eubacterium Salinibacter ruber. These organisms live in highly halophilic environments, suggesting the possibility of the effects of salts on the function of SRI. However, such effects remain unclear because SRI proteins from H. salinarum (HsSRI) are unstable in dilute salt solutions. Recently, we characterized a new SRI protein (SrSRI) that is stable even in the absence of salts, thus allowing us to investigate the effects of salts on the photochemical properties of SRI. In this study, we report that the absorption maximum of SrSRI is shifted from 542 to 556 nm in a Cl−-dependent manner with a Km of 307 ± 56 mM, showing that Cl−-binding sites exist in SRI. The bathochromic shift was caused not only by NaCl but also by other salts (NaI, NaBr, and NaNO3), implying that I−, Br−, and NO3− can also bind to SrSRI. In addition, the photochemical properties during the photocycle are also affected by chloride ion binding. Mutagenesis studies strongly suggested that a conserved residue, His131, is involved in the Cl−-binding site. In light of these results, we discuss the effects of the Cl− binding to SRI and the roles of Cl− binding in its function. 相似文献
85.
Shinji Ohsawa Takeshi Saito Shin Yoshikawa Hideo Mawatari Makoto Yamada Kazuhiro Amita Nobuki Takamatsu Yasuaki Sudo Tsuneomi Kagiyama 《Limnology》2010,11(3):207-215
One feature of volcanic lakes influenced by subaqueous fumaroles existing at lake bottoms (called active crater lakes) is
the remarkable color of their waters: turquoise or emerald green. The active crater lake named Yudamari at Mt. Nakadake of
Aso volcano, Japan, takes on a milky pale blue-green. The particular blue component of the lake water color results from Rayleigh
scattering of sunlight by very fine aqueous colloidal sulfur particles; the green component is attributable to absorption
of sunlight by dissolved ferrous ions. An objective color observation conducted during 2000–2007 revealed that the lake water
color changed from blue-green to solid green. The disappearance of the blue ingredient of the water color will result in diminution
of aqueous colloidal sulfur from chemical analyses of lake waters sampled simultaneously. The aqueous sulfur is produced by
the reaction of sulfur dioxide and hydrogen sulfide supplied from subaqueous fumaroles. However, its production efficiency
decreases by domination of sulfur dioxide in the subaqueous fumarolic sulfur gas species with increasing subaqueous fumarolic
temperature. The disappearance of blue ingredients from the blue-green color of the lake water may be attributed to activation
of subaqueous fumarole activity. 相似文献
86.
Landgraf SS Wengert M Silva JS Zapata-Sudo G Sudo RT Takiya CM Pinheiro AA Caruso-Neves C 《American journal of physiology. Renal physiology》2011,300(2):F499-F510
The renal renin-angiotensin system plays a central role in the development of hypertension. The aim of this work was to verify the expression of angiotensin II receptors AT(1)R and AT(2)R in the microsomal fraction of renal cortex and correlate this with the development of hypertension and renal damage in spontaneously hypertensive rats (SHR) using Wistar-Kyoto rats (WKY) as controls. AT(1)R expression increased (126%) and AT(2)R expression decreased (66%) in 4-wk-old SHR; AT(2) expression decreased in 14-wk-old SHR (61%) compared with respective age-matched WKY. These modifications were correlated to the increase in protein kinase C activity and decrease in protein kinase A activity. Four-week-old SHR showed large accumulations of macrophages in kidney glomerulus and the tubulointerstitial area, dense cortical collagen deposition, and arterial proliferative changes in the walls of arterioles and medium-sized vessels. Similar modifications were also observed in 14-wk-old SHR. Four-week-old SHR treated with losartan (30 mg·kg(-1)·day(-1)) or hydralazine (15 and 30 mg·kg(-1)·day(-1)) by gavage for 10 wk did not develop hypertension. The decrease in AT(2)R expression and renal damage observed in SHR remained even after treatment with hydralazine. On the other hand, losartan treatment prevented the modifications observed in 14-wk-old SHR, indicating that renal injuries are caused specifically by AT(1) rather than an increase in blood pressure. Our results indicate that the imbalance in AT(1)R and AT(2)R expression is associated with an inflammatory process that contributes to renal injury in adult SHR and to the development of hypertension. 相似文献
87.
The adaxial (upper) and abaxial (lower) surfaces of a plant leaf provide heterogeneous habitats for small arthropods with
different environmental conditions, such as light, humidity, and surface morphology. As for plant mites, some agricultural
pest species and their natural enemies have been observed to favor the abaxial leaf surface, which is considered an adaptation
to avoid rain or solar ultraviolet radiation. However, whether such a preference for the leaf underside is a common behavioral
trait in mites on wild vegetation remains unknown. The authors conducted a 2-year survey on the foliar mite assemblage found
on Viburnum erosum var. punctatum, a deciduous shrub on which several mite taxa occur throughout the seasons, and 14 sympatric tree or shrub species in secondary
broadleaf-forest sites in Kyoto, west–central Japan. We compared adaxial–abaxial surface distributions of mites among mite
taxa, seasons, and morphology of host leaves (presence/absence of hairs and domatia). On V. erosum var. punctatum, seven of 11 distinguished mite taxa were significantly distributed in favor of abaxial leaf surfaces and the trend was seasonally
stable, except for Eriophyoidea. Mite assemblages on 15 plant species were significantly biased towards the abaxial leaf surfaces,
regardless of surface morphology. Our data suggest that many mite taxa commonly prefer to stay on abaxial leaf surfaces in
wild vegetation. Oribatida displayed a relatively neutral distribution, and in Tenuipalpidae, the ratio of eggs collected
from the adaxial versus the abaxial side was significantly higher than the ratio of the motile individuals, implying that
some mite taxa exploit adaxial leaf surfaces as habitat. 相似文献
88.
Kazufumi Yoshihara Tetsuya Hiramoto Takakazu Oka Chiharu Kubo Nobuyuki Sudo 《BioPsychoSocial medicine》2014,8(1):1-9
Background
Previous studies have shown that the practice of yoga reduces perceived stress and negative feelings and that it improves psychological symptoms. Our previous study also suggested that long-term yoga training improves stress-related psychological symptoms such as anxiety and anger. However, little is known about the beneficial effects of yoga practice on somatization, the most common stress-related physical symptoms, and stress-related biomarkers. We performed a prospective, single arm study to examine the beneficial effects of 12 weeks of yoga training on somatization, psychological symptoms, and stress-related biomarkers.Methods
We recruited healthy women who had no experience with yoga. The data of 24 participants who were followed during 12 weeks of yoga training were analyzed. Somatization and psychological symptoms were assessed before and after 12 weeks of yoga training using the Profile of Mood State (POMS) and the Symptom Checklist-90-Revised (SCL-90-R) questionnaires. Urinary 8-hydroxydeoxyguanosine (8-OHdG), biopyrrin, and cortisol levels were measured as stress-related biomarkers. The Wilcoxon signed-rank test was used to compare the stress-related biomarkers and the scores of questionnaires before and after 12 weeks of yoga training.Results
After 12 weeks of yoga training, all negative subscale scores (tension-anxiety, depression, anger-hostility, fatigue, and confusion) from the POMS and somatization, anxiety, depression, and hostility from the SCL-90-R were significantly decreased compared with those before starting yoga training. Contrary to our expectation, the urinary 8-OHdG concentration after 12 weeks of yoga training showed a significant increase compared with that before starting yoga training. No significant changes were observed in the levels of urinary biopyrrin and cortisol after the 12 weeks of yoga training.Conclusions
Yoga training has the potential to reduce the somatization score and the scores related to mental health indicators, such as anxiety, depression, anger, and fatigue. The present findings suggest that yoga can improve somatization and mental health status and has implications for the prevention of psychosomatic symptoms in healthy women.Trial registration
University Hospital Medical Information Network (UMIN CTR) UMIN000007868. 相似文献89.
Makoto Sudo Yoshiki Yamaguchi Peter J. Sp?th Kana Matsumoto-Morita Benjamin K. Ong Nortina Shahrizaila Nobuhiro Yuki 《PloS one》2014,9(9)
Intravenous immunoglobulin (IVIG) is the first line treatment for Guillain–Barré syndrome and multifocal motor neuropathy, which are caused by anti-ganglioside antibody-mediated complement-dependent cytotoxicity. IVIG has many potential mechanisms of action, and sialylation of the IgG Fc portion reportedly has an anti-inflammatory effect in antibody-dependent cell-mediated cytotoxicity models. We investigated the effects of different IVIG glycoforms on the inhibition of antibody-mediated complement-dependent cytotoxicity. Deglycosylated, degalactosylated, galactosylated and sialylated IgG were prepared from IVIG following treatment with glycosidases and glycosyltransferases. Sera from patients with Guillain–Barré syndrome, Miller Fisher syndrome and multifocal motor neuropathy associated with anti-ganglioside antibodies were used. Inhibition of complement deposition subsequent to IgG or IgM autoantibody binding to ganglioside, GM1 or GQ1b was assessed on microtiter plates. Sialylated and galactosylated IVIGs more effectively inhibited C3 deposition than original IVIG or enzyme-treated IVIGs (agalactosylated and deglycosylated IVIGs). Therefore, sialylated and galactosylated IVIGs may be more effective than conventional IVIG in the treatment of complement-dependent autoimmune diseases. 相似文献
90.
Toshihiko Sudo Xiaoxian Zhao Yoko Wakamatsu Miki Shibahara Nobuhiko Nomura Tadaatsu Nakahara Akemi Suzuki Yoshiro Kobayashi Chunyuan Jin Takehide Murata Kazunari K. Yokoyama 《Cytotechnology》2000,33(1-3):259-264
Four analogs of succinoyl trehalose lipid-3 (STL-3)with saturated even-number or odd-number carbonchains, and unsaturated or halogenated fatty acidswere examined for their ability to inhibit the growthand induce the differentiation of HL-60 humanpromyelocytic leukemia cells. The optimalconcentration of STL-3 at which such activities wererecognized was closed to the critical micelleconcentration of STL-3. Analog of STL-3 witheven-number or odd-number carbon chain and unsaturatedfatty acids strongly inhibited growth and induced thedifferentiation of HL-60 cells, as evaluated in termsof nitroblue tetrazilium-reducing activity and theappearance of the CD36 antigen. An analog of STL-3with halogenated fatty acids significantly inhibitedproliferation but only induced the differentiation ofHL-60 cells. Our results indicate that the effects ofSTL-3 and its analogs on HL-60 cells depend on thestructure of the hydrophobic moiety of STL-3.These authors contributed equally to this work 相似文献