全文获取类型
收费全文 | 457篇 |
免费 | 35篇 |
出版年
2023年 | 1篇 |
2022年 | 1篇 |
2021年 | 4篇 |
2020年 | 4篇 |
2019年 | 7篇 |
2018年 | 6篇 |
2017年 | 6篇 |
2016年 | 11篇 |
2015年 | 21篇 |
2014年 | 18篇 |
2013年 | 39篇 |
2012年 | 32篇 |
2011年 | 27篇 |
2010年 | 21篇 |
2009年 | 14篇 |
2008年 | 25篇 |
2007年 | 37篇 |
2006年 | 28篇 |
2005年 | 32篇 |
2004年 | 22篇 |
2003年 | 19篇 |
2002年 | 23篇 |
2001年 | 6篇 |
2000年 | 4篇 |
1999年 | 5篇 |
1998年 | 7篇 |
1997年 | 10篇 |
1996年 | 7篇 |
1995年 | 6篇 |
1994年 | 3篇 |
1993年 | 3篇 |
1992年 | 3篇 |
1991年 | 6篇 |
1990年 | 2篇 |
1989年 | 3篇 |
1987年 | 3篇 |
1986年 | 2篇 |
1984年 | 6篇 |
1982年 | 2篇 |
1981年 | 2篇 |
1978年 | 1篇 |
1977年 | 3篇 |
1976年 | 1篇 |
1975年 | 2篇 |
1974年 | 5篇 |
1968年 | 2篇 |
排序方式: 共有492条查询结果,搜索用时 15 毫秒
211.
Motivated by recent experimental work of Burd et al., we propose a model of bi-directional ant traffic on pre-existing ant trails. It captures in a simple way some of the generic collective features of movements of real ants on a trail. Analysing this model, we demonstrate that there are crucial qualitative differences between vehicular- and ant-traffics. In particular, we predict some unusual features of the flow rate that can be tested experimentally. As in the uni-directional model a non-monotonic density-dependence of the average velocity can be observed in certain parameter regimes. As a consequence of the interaction between oppositely moving ants the flow rate can become approximately constant over some density interval. 相似文献
212.
Ricci-Silva ME Konno K Faria F Rádis-Baptista G Fontes W Stöcklin R Michalet S Yamane T Chudzinski-Tavassi AM 《Omics : a journal of integrative biology》2005,9(2):194-208
The salivary complex of leeches contains many components able to modulate physiological mechanisms, such as coagulation and fibrinolysis, and it is composed by the salivary glands and proboscis, encompassing two different proteomes. The bidimensional electrophoretic pattern of the salivary complex from the Haementeria depressa leech revealed a total of 352 spots, 103 in common with the muscular tissue and 249 exclusive from the salivary complex as detected by silver staining; these spots showed isoelectric points from 3.5 to 9.5 and covered an apparent molecular weight range from 10 to 105 kDa. The following isoforms of proteins were identified by mass spectrometry analysis: antiplatelet protein, myohemerythrin and carbonic anhydrase. Since the leeches were not fed for about 2-3 months to stimulate the secretion of proteins that facilitates the blood metabolism, these most abundant proteins in the salivary complex excised from leeches, are expected to play a role during feeding and might have some anti-hemostatic properties. Furthermore, by zymography, a gelatinolytic and a fibrinolytic protein were identified. 相似文献
213.
Characterization of Hexose Transporter for Facilitated Diffusion of the Tonoplast Vesicles from Pear Fruit 总被引:2,自引:0,他引:2
Tonoplast vesicles were prepared from the flesh tissue of maturepear fruit. Sugar uptakes into the vesicles determined by twodifferent methods, the membrane and the gel filtration methods,were quite similar. The uptake was highest for glucose and subsequently,in order, for fructose, sucrose and sorbitol. It was not stimulatedby addition of ATP, although the vesicles could create a protongradient. However, the uptakes were significantly inhibitedby p-chloromercuribenzene sulphonate (PCMBS, SH-reagent andinhibitor of sugar transporter). Further, the PCMBS-sensitiveuptakes of glucose and fructose saturated with their increasedconcentrations. Thus, these PCMBS-sensitive uptakes are mediatedby the transporter of facilitated diffusion. The uptakes ofglucose or fructose each had two Km values. Km values for glucosewere 0.35 and 18 mM, and those for fructose were 1.6 and 25raM. The uptake of 0.2 mM glucose was inhibited by 2 mM fructoseand that of 2 mM fructose was inhibited by 2 mM glucose, butneither was inhibited by sucrose or sorbitol. O-methyl-glucose(OMG) also inhibited both the glucose and fructose uptakes.Therefore, the same transporter may mediate both glucose andfructose uptakes at lower concentrations; this hexose transportsystem differed from the sucrose and sorbitol transport systems.
1Research Fellow of the Japan Society for the Promotion of Science.
2Present address: Faculty of Agriculture, Tohoku University,1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai, 981 Japan. 相似文献
214.
Life tables for two predatory ladybirds,Coccinella septempunctata andHarmonia axyridis, were constructed in two years in which prey abundance differed. The prey aphid,Aphis gossypii, was abundant on its primary host from late May to late June. Females ofH. axyridis andC. septempumctata laid their eggs during the increase and peak in aphid abundance. The oviposition period ofC. septempunctata started before and was shorter than that ofH. axyridis. In both species, intraguild predation and/or cannibalism of the egg and fourth instar stages, but rarely of other developmental stages, were observed. Fourth instar larvae ofH. axyridis had to complete their development when aphids were scarce more frequently than those ofC. septempunctata. The highest frequency of intra- and interspecific predation was of fourth instarH. axyridis larvae. In terms of intraguild predation, the larvae ofH. axyridis preyed on larvae ofC. septempunctata, but the reverse was not observed. Percentage survival from egg to adult inH. axyridis was higher than inC. septempunctata in both years, and least in both species in the year of low aphid abundance. These results suggest that prey abundance influenced the frequency of cannibalism and intraguild predation, which were important in structuring this guild of ladybirds. 相似文献
215.
Pressure shock treatment induced synthesis of heat shock protein (hsp104) and tolerance against various stresses such as high temperature, high pressure and high concentration of ethanol in Saccharomyces cerevisiae. The optimum pressures that induced maximal tolerance against these stresses were in the range of 50–75 MPa and depended on the type of stress. However, pressure shock did not stimulate trehalose production in the cells. © Rapid Science Ltd. 1998 相似文献
216.
Katsuhiro Fukuta Hiroshi Kudo N. H. Fuzina S. Sivanandam A. R. Mutlib S. Jalaludin 《Primates; journal of primatology》1994,35(1):105-108
Thirteen days after capture, the blood serum of eight anesthetized female leaf-monkeys,Presbytis cristatus (3.86 kg of mean body weight), were analyzed for hematocrit (35.6±6.7 %), total protein (6.7±0.8 g/dl), albumin (3.61±0.77
g/dl), α-1 globulin (0.13±0.04 g/dl), α-2 globulin (0.73±0.18 g/dl), β globulin (0.87±0.27 g/dl), γ globulin (1.36±0.55 g/dl),
A/G ratio (1.23±0.38), Na (161±6.14 mEq/l), K (5.61±0.74 mEq/l), LDH (575±257 IU/l), GOT (93±67 IU/l), GPT (34±33 IU/l), CPK
(250±200 IU/l), ALP (613±633 IU/l), LAP (115±68 IU/l), γ-GTP (28±30 IU/l), TG (47±24 mg/dl), T-Cho (141±31 mg/dl), BUN (29.0±5.7
mg/dl), T-bil (0.21±0.07 mg/dl), and IP (3.4±2.0 mg/dl). 相似文献
217.
Yoshiaki Takahashi Kohnosuke Mitani Katsuhiro Kuwabara Tatsuhiko Hayashi Michiko Niwa Nobumoto Miyashita Kazuo Moriwaki Ryo Kominami 《Chromosoma》1994,103(7):450-458
Mouse mo-2 macrosatellites consisting of 31-bp tandem repeat units are mainly located at two loci in the C57BL/6 genome, one being at the centromere-distal telomeric region of chromosome 9 and the other at the pseudoautosomal (PA) region of chromosomes X and Y. The two clustes constitute approximately 300 kb and 150 kb, respectively. Southern analysis of a methylation-sensitive enzyme, HpaII-digested DNA showed that the mo-2 macrosatellites are detected as more than 30 polymorphic bands. Comparison of those bands between reciprocally crossed F1 mice revealed that approximately 20% of the allele-specific fragments exhibit different band intensities depending on the sex of the parent of origin. The differential methylation is observed in the mo-2 macrosatellite on the PA region but not in that on chromosome 9. Several fragments including the 3.4-kb fragment without internal HpaII site are more clearly detected when paternally derived, suggesting that the male-derived macrosatellite is undermethylated. Interestingly the difference is much more remarkable in inter-subspecific F1 mice between C57BL/6 and MSM than F1 between C57BL/6 and C3H/He. This suggests the presence of a modifier(s) that affect(s) the methylation of mo-2 in the MSM genome. 相似文献
218.
219.
Temozolomide regresses a doxorubicin‐resistant undifferentiated spindle‐cell sarcoma patient‐derived orthotopic xenograft (PDOX): precision‐oncology nude‐mouse model matching the patient with effective therapy
下载免费PDF全文
![点击此处可从《Journal of cellular biochemistry》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Kentaro Igarashi Kei Kawaguchi Tasuku Kiyuna Kentaro Miyake Masuyo Miyake Yunfeng Li Scott D. Nelson Sarah M. Dry Arun S. Singh Irmina A. Elliott Tara A. Russell Mark A. Eckardt Norio Yamamoto Katsuhiro Hayashi Hiroaki Kimura Shinji Miwa Hiroyuki Tsuchiya Fritz C. Eilber Robert M. Hoffman 《Journal of cellular biochemistry》2018,119(8):6598-6603
220.
Cytoplasm and cell sap of Lamprothamnium succinctum were analyzedseparately for the contents of free amino acids and sucroseto find whether they contribute to turgor regulation. In thevacuole, both amino acids and sucrose were found to be minorcomponents contributing to the generation of osmotic pressure.Their amounts were almost insensitive to changes in externalosmotic pressure. In the cytoplasm, both amino acids and sucrosein the cytoplasm contributed about 20% to the osmotic pressure.Hypotonic treatment did not affect the contents of either, buthypertonic treatment, while not affecting the amino acid contents,caused a significant increase in sucrose content. The cytoplasmicsucrose content increased linearly with an increase in externalosmotic pressure, accounting for 40% of the increased osmoticpressure.
1 Present address: Department of Biology, Osaka Medical College,Sawaragi-cho, Takatsuki, Osaka 569, Japan
2 Present address: Department of Applied Physiology, NationalInstitute of Agrobiological Resources, Yatabe, Tsukuda, Ibaragi305, Japan (Received November 25, 1986; Accepted March 18, 1987) 相似文献