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71.
72.
Concerted transpositions of mobile genetic elements coupled with fitness changes in Drosophila melanogaster 总被引:3,自引:0,他引:3
Pasyukova EG; Belyaeva ES; Kogan GL; Kaidanov LZ; Gvozdev VA 《Molecular biology and evolution》1986,3(4):299-312
In an inbred low-activity (LA) strain of Drosophila melanogaster with a low
level of fitness and a complex of inadaptive characters, in situ
hybridization reveals an invariant pattern of distribution of three
copia-like elements (mdg-1, mdg-3, and copia). Rare, spontaneous, multiple
transpositions of mobile elements in the LA strain were shown to be coupled
with a drastic increase of fitness. A changed pattern of various types of
mobile elements was also observed on selecting the LA strain for higher
fitness. High-fitness strains show transpositions of mobile elements to
definite chromosomal sites ("hot spots"). Concerted changes in the location
of three different mobile elements were found to be coupled with an
increase of fitness. The mdg-1 distribution patterns were also examined in
two low-fitness strains independently selected from the high-fitness ones.
Fitness decrease was accompanied by mdg-1 excision from the hot spots of
their location usually detected in the high-fitness strains. The results
suggest the existence of a system of adaptive transpositions of mobile
elements that takes part in fitness control.
相似文献
73.
The circular dichroism spectra of liquid-crystalline dispersions obtained by phase exclusion of linear double-stranded DNA molecules from aqueous saline solutions of polyethylene glycol (120 ≤ CPEG ≤ 300 mg/mL) have been investigated. The formation of liquid-crystalline dispersions at polyethylene glycol concentrations ranging from 120 to 200 mg/mL was accompanied by the emergence of an abnormal negative band in the spectrum of circular dichroism; this is indicative of cholesteric packing of the double stranded DNA molecules in the particles of the dispersion. Liquid-crystalline dispersions formed at PEG concentrations higher than 220 mg/mL and room temperature did not show any abnormal bands in the circular dichroism spectra; this is indicative of hexagonal packing of double-stranded DNA molecules in the particles of the dispersions. Heating of optically inactive liquid crystal dispersions induced a transition of the dispersions into a different state accompanied by the emergence of an abnormal negative band in the spectrum of circular dichroism. This transition is considered within the concept of the transformation of a hexagonal packing of DNA molecules into a cholesteric packing. A qualitative mechanism of such a transition is proposed that is formulated in the terms of the “quasinematic” layers of double-stranded DNA molecules that change their spatial orientation under the competing influences of the osmotic pressure of the solvent, orientational elasticity of the cholesteric packing, and thermal fluctuations. 相似文献
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L E Klazacheva I S Tartakovski? N D Konstantinova R A Pshenichnov L N Kats 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》1977,(9):131-133
A study was made of the structure of Bac. subtilis and Listeria monocytogenes protoplasts by the method of scanning electron microscopy. The mechanism of protoplast formation in Gram-positive bacteria and in spheroplasts of Gram-negative bacteria proved to differ. A loss of the rigid form of the cell, round protrusions on cell surface, and an escape of the cytoplasm through the ruptured cell wall in some one place was noted in case of protoplasts. Individual cells can coalesce with one another with the formation of shapeless masses. The formation of small spheroid bodies by budding, and also a division of protoplasts by constriction was described. 相似文献
79.
There was shown a difference in the biological properties and the ultrastructure of two strains of brucellae, spheroplasts obtained from them under the action of penicillin, L-form and revertants obtained from the L-form. Spheroplasts formation was characterized by a change of brucellae into R-form and some virulence reduction. The cells had an outer and a cytoplasmic membranes, and usually lost their capacity to binary division. L-forms were obtained during the 9th and the 35th passage on a medium with penicillin; their formation was accompanied by the change in serological properties of the culture and significant reduction of the virulence; the cells were characterized by a marked polymorphism and the capacity to budding; they had 2 membranes on the cell surface and an intensively developed system of intracytoplasmic membranes. The revertants formed on the medium without penicillin during the 16th-30th passage or spontaneously on the medium with penicillin. They differed from the initial strains of brucella culture by a marked increase in penicillin-resistance, by the changes in serological properties, and also by polymorphism of cells, capable, however, of binary division. 相似文献
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