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961.
962.
Cristobal A. Onetto Kathryn L. Eales Paul Guagliardo Matt R. Kilburn Joanna M. Gambetta Paul R. Grbin 《Systematic and applied microbiology》2017,40(8):500-507
Defluviicoccus vanus-related glycogen accumulating organisms (GAO) regularly proliferate in industrial wastewater treatment plants handling high carbon but nitrogen deficient wastes. When GAO dominate, they are associated with poor performance, characterised by slow settling biomass and turbid effluents. Although their ecophysiology has been studied thoroughly in domestic waste treatment plants, little attention has been paid to them in aerobic industrial systems.In this study, the effect of nitrogen addition on GAO carbon metabolism was investigated during an 8 h cycle. Activated sludge dominated by GAO from a winery wastewater sequencing batch reactor was incubated under different carbon to nitrogen (COD:N) ratios (100:1, 60:1 and 20:1) using 13C — acetate and 15N — urea. GAO cell assimilation was quantified using FISH-NanoSIMS. The activated sludge community was assessed by 16S rRNA gene profiling, DNA and storage polymer production. Carbon and nitrogen quantification at the cellular level by NanoSIMS revealed that low (COD:N of 100:1) or null nitrogen concentrations enhanced GAO carbon uptake. COD:N ratios of 60:1 and 20:1 reduced GAO carbon uptake and promoted whole microbial community DNA production. Nitrogen dosing at COD:N ratios of 60:1 or higher was demonstrated as feasible strategy for controlling the excessive GAO growth in high COD waste treatment plants. 相似文献
963.
Kathryn L. Garner Rebecca M. Perrett Margaritis Voliotis Clive Bowsher George R. Pope Thanh Pham Christopher J. Caunt Krasimira Tsaneva-Atanasova Craig A. McArdle 《The Journal of biological chemistry》2016,291(5):2246-2259
Cell signaling pathways are noisy communication channels, and statistical measures derived from information theory can be used to quantify the information they transfer. Here we use single cell signaling measures to calculate mutual information as a measure of information transfer via gonadotropin-releasing hormone (GnRH) receptors (GnRHR) to extracellular signal-regulated kinase (ERK) or nuclear factor of activated T-cells (NFAT). This revealed mutual information values <1 bit, implying that individual GnRH-responsive cells cannot unambiguously differentiate even two equally probable input concentrations. Addressing possible mechanisms for mitigation of information loss, we focused on the ERK pathway and developed a stochastic activation model incorporating negative feedback and constitutive activity. Model simulations revealed interplay between fast (min) and slow (min-h) negative feedback loops with maximal information transfer at intermediate feedback levels. Consistent with this, experiments revealed that reducing negative feedback (by expressing catalytically inactive ERK2) and increasing negative feedback (by Egr1-driven expression of dual-specificity phosphatase 5 (DUSP5)) both reduced information transfer from GnRHR to ERK. It was also reduced by blocking protein synthesis (to prevent GnRH from increasing DUSP expression) but did not differ for different GnRHRs that do or do not undergo rapid homologous desensitization. Thus, the first statistical measures of information transfer via these receptors reveals that individual cells are unreliable sensors of GnRH concentration and that this reliability is maximal at intermediate levels of ERK-mediated negative feedback but is not influenced by receptor desensitization. 相似文献
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967.
Do skeletal muscle phenotypic characteristics of Xhosa and Caucasian endurance runners differ when matched for training and racing distances? 总被引:1,自引:0,他引:1
Tertius A Kohn Birgitta Essén-Gustavsson Kathryn H Myburgh 《Journal of applied physiology》2007,103(3):932-940
Although East African black athletes dominate endurance running events, it is unknown whether black and white endurance runners with similar racing ability, matched for training, may differ in their skeletal muscle biochemical phenotype. Thirteen Xhosa (XR) and 13 Caucasian (CR) endurance runners were recruited and matched for 10-km performance, average preferred racing distance (PRD(A)), and training volume. Submaximal and maximal exercise tests were done, and vastus lateralis muscle biopsies were taken. XR were significantly lighter and shorter than CR athletes but had similar maximum oxygen consumption corrected for body weight and peak treadmill speed (PTS). XR had lower plasma lactate concentrations at 80% PTS (P < 0.05) compared with CR. Also, XR had more type IIA (42.4 +/- 9.2 vs. 31.3 +/- 11.5%, P < 0.05) and less type I fibers (47.8 +/- 10.9 vs. 63.1 +/- 13.2%, P < 0.05), although oxidative enzyme activities did not differ. Furthermore, XR compared with CR had higher lactate dehydrogenase (LDH) activity in homogenate muscle samples (383 +/- 99 vs. 229 +/- 85 mumol.min(-1).g dry weight(-1), P < 0.05) and in both type IIa (P < 0.05) and type I (P = 0.05) single-fiber pools. A marked difference (P < 0.05) in the composition of LDH isoform content was found between the two groups with XR having higher levels of LDH(5-4) isoforms (skeletal muscle isozymes; LDH-M) than CR, which was not accounted for by fiber-type differences alone. These results confirm differences in muscle phenotype and physiological characteristics, particularly associated with high-intensity running. 相似文献
968.
Dunkley Katie Ellison Amy R. Mohammed Ryan S. van Oosterhout Cock Whittey Kathryn E. Perkins Sarah E. Cable Jo 《Coral reefs (Online)》2019,38(2):321-330
Coral Reefs - Cleaning interactions, which involve a cleaner removing ectoparasites and other material from the body of a heterospecific (client), are iconic symbiotic interactions observed on... 相似文献
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Richard Copin Alina Baum Elzbieta Wloga Kristen E. Pascal Stephanie Giordano Benjamin O. Fulton Anbo Zhou Nicole Negron Kathryn Lanza Newton Chan Angel Coppola Joyce Chiu Min Ni Yi Wei Gurinder S. Atwal Annabel Romero Hernandez Kei Saotome Yi Zhou Christos A. Kyratsous 《Cell》2021,184(15):3949-3961.e11
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