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91.
92.
Role of siderophores in the biocontrol of Pseudomonas tolaasii by fluorescent pseudomonad antagonists 总被引:1,自引:1,他引:0
A number of pseudomonad bacteria were investigated for their in vitro antagonism on agar against Pseudomonas tolaasii , the causative agent of bacterial blotch of the cultivated mushroom. Addition of FeCl3 to the culture medium suppressed the antagonism in 14 of the 20 bacteria tested and the production of a substance with an absorption peak at 400 nm by all antagonists was eliminated by the presence of Fe3+ . Both observations indicated that siderophores could be involved in the mode of action of some antagonists. The addition of the iron chelators EDTA and bipyridyl to the medium used for culture of antagonists and pathogens did not generally prevent growth. Siderophore production is not essential for the in vivo activity of antagonists. 相似文献
93.
Molecular cloning and expression of a cDNA encoding endothelial cell nitric oxide synthase. 总被引:31,自引:0,他引:31
W C Sessa J K Harrison C M Barber D Zeng M E Durieux D D D'Angelo K R Lynch M J Peach 《The Journal of biological chemistry》1992,267(22):15274-15276
Endothelium-derived relaxing factor (EDRF), identified as nitric oxide (NO), is derived from a guanidino nitrogen of L-arginine via its metabolism by nitric oxide synthase (NOS). Herein, we report the molecular cloning of a cDNA encoding the constitutive calcium-calmodulin (Ca2+/CaM)-regulated nitric oxide synthase (ECNOS). A full-length ECNOS clone was isolated by screening a bovine aortic endothelial cell cDNA library using a fragment of rat brain NOS (bNOS) cDNA. This cDNA has an open reading frame of 3615 nucleotides encoding a 1205-amino acid protein. Membranes prepared from COS cells transfected with the ECNOS cDNA demonstrated NADPH- and Ca2+/CaM- dependent conversion of L-, but not D-, arginine to NO and citrulline that was inhibited by NG-nitro-L-arginine methyl ester. Comparison of the deduced amino acid sequence of ECNOS to the bNOS and macrophage NOS (Mac-NOS) sequences revealed 57 and 50% identity, respectively. In addition, ECNOS contains a unique N-myristylation consensus sequence (not shared by bNOS or Mac-NOS) that may explain its membrane localization. 相似文献
94.
95.
Regulation by estrogen through the 5'-flanking region of the transforming growth factor alpha gene. 总被引:2,自引:0,他引:2
T Saeki A Cristiano M J Lynch M Brattain N Kim N Normanno N Kenney F Ciardiello D S Salomon 《Molecular endocrinology (Baltimore, Md.)》1991,5(12):1955-1963
Expression of transforming growth factor alpha (TGF alpha) mRNA and protein can be stimulated by estrogens such as 17 beta-estradiol (E2) in estrogen-responsive rodent and human breast cancer cells. To ascertain if E2 can directly regulate TGF alpha expression through the 5'-flanking region of the human TGF alpha gene, E2-responsive MCF-7 or ZR-75-1 human breast cancer cells or E2-nonresponsive MDA-MB-231 breast cancer cells were transiently transfected with a plasmid containing an 1140-base pair (bp) Sac-I fragment of the TGF alpha 5'-flanking region ligated to the chloramphenicol acetyltransferase (CAT) gene. Cells that were transfected and subsequently treated with physiological concentrations of E2 (10(-11)-10(-8) M) for 24 h exhibited a 2- to 10-fold increase in CAT activity. The E2 stimulation of CAT activity was dose-dependent with an increase first found at 10(-10) M E2. The increase in CAT activity could be detected within 24-36 h after the addition of E2. There was no significant change in CAT activity in transiently transfected MDA-MB-231 cells as mediated through the TGF alpha 5'-flanking region after E2 treatment. MCF-7 cells were also transiently transfected with different fragments of the TGF alpha 5'-flanking region ligated to the luciferase gene. In the absence of E2 treatment, no detectable luciferase activity was found.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
96.
97.
Michael Lynch Wilfried Gabriel 《Evolution; international journal of organic evolution》1990,44(7):1725-1737
Previous attempts to model the joint action of selection and mutation in finite populations have treated population size as being independent of the mutation load. However, the accumulation of deleterious mutations is expected to cause a gradual reduction in population size. Consequently, in small populations random genetic drift will progressively overpower selection making it easier to fix future mutations. This synergistic interaction, which we refer to as a mutational melt-down, ultimately leads to population extinction. For many conditions, the coefficient of variation of extinction time is less than 0.1, and for species that reproduce by binary fission, the expected extinction time is quite insensitive to population carrying capacity. These results are consistent with observations that many cultures of ciliated protozoans and vertebrate fibroblasts have characteristic extinction times. The model also predicts that clonal lineages are unlikely to survive more than 104 to 105 generations, which is consistent with existing data on parthenogenetic animals. Contrary to the usual view that Muller's ratchet does more damage when selection is weak, we show that the mean extinction time declines as mutations become more deleterious. Although very small sexual populations, such as self-fertilized lines, are subject to mutational meltdowns, recombination effectively eliminates the process when the effective population size exceeds a dozen or so. The concept of the effective mutation load is developed, and several procedures for estimating it are described. It is shown that this load can be reduced substantially when mutational effects are highly variable. 相似文献
98.
Fibroblast heterogeneity in glucocorticoid regulation of collagen metabolism: Genetic or epigenetic?
James D. Russell Shirley B. Russell Kathryn M. Trupin 《In vitro cellular & developmental biology. Plant》1982,18(6):557-564
Summary Cultured fibroblasts derived from normal human dermis show a consistent 62% inhibition of collagen synthesis by hydrocortisone,
whereas cultures derived from keloids average only 30% inhibition and show a much larger strain to strain variation ranging
from 75% inhibition to 49% stimulation. Examination of fibroblast clones indicates that this high variation among keloid strains
is not due to differences in the proportion of normal and keloid cells in the mass culture populations. Small but significant
differences in the effect of hydrocortisone on collagen deposition are also seen among these clonal populations, but are not
related to the type of tissue from which cultures were derived. Two to three-fold differences among clones derived from a
single individual were observed, possibly suggesting functional heterogeneity of dermal fibroblasts with regard to collagen
metabolism under control conditions and in response to hydrocortisone. However, this variation among clones may simply reflect
differences in clonal growth, inasmuch as both collagen synthesis and deposition, and the effect of hydrocortisone on these
processes, are strongly affected by population density.
This work was supported in part by PHS grants, CA-17229 from the National Cancer Institute and AG-02046 from the National
Institute on Aging, DHHS; and by Grant RIM 78-17313 from the National Science Foundation. 相似文献
99.
100.
The postneonatal death rate was studied for 332 infants from 160 families, ascertained through an abused proband. There were nine deaths compared with 2.9 expected from the legitimacy, social class, age, and parity distribution (p = 0.003). All but one of the babies died at home, and all were referred to a coroner or procurator fiscal. No adequate explanation of death could be found in four cases. Bonding problems probably existed in most of the nine families before death occurred. 相似文献