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991.
Summary Numerous plasmodesmata occur in the walls between the secretory cells ofTamarix salt glands. The plasmalemma bounds the plasmodesmata and is continuous from cell to cell. In freeze-fracture, the e-face of the plasmalemma within the plasmodesmata is virtually devoid of intramembranous particles while, in contrast, the p-face is decidedly enriched with particles. The axial components appear to be a tightly curved membrane bilayer, as judged from measurements and their appearance in freeze-fracture, and the e-face of this membrane is also devoid of particles. Observations from both thin sections and freeze-fracture replicas indicate the presence of a circular cluster of six particles around the axial component near the cytoplasmic termini of the plasmodesmata. These particles extend from the p-face of the axial component to the p-face of the plasmalemma. These observations are summarized in a model. 相似文献
992.
Increased transport of acetyl‐CoA into the endoplasmic reticulum causes a progeria‐like phenotype 下载免费PDF全文
Yajing Peng Samantha L. Shapiro Varuna C. Banduseela Inca A. Dieterich Kyle J. Hewitt Emery H. Bresnick Guangyao Kong Jing Zhang Kathryn L. Schueler Mark P. Keller Alan D. Attie Timothy A. Hacker Ruth Sullivan Elle Kielar‐Grevstad Sebastian I. Arriola Apelo Dudley W. Lamming Rozalyn M. Anderson Luigi Puglielli 《Aging cell》2018,17(5):e12820
The membrane transporter AT‐1/SLC33A1 translocates cytosolic acetyl‐CoA into the lumen of the endoplasmic reticulum (ER), participating in quality control mechanisms within the secretory pathway. Mutations and duplication events in AT‐1/SLC33A1 are highly pleiotropic and have been linked to diseases such as spastic paraplegia, developmental delay, autism spectrum disorder, intellectual disability, propensity to seizures, and dysmorphism. Despite these known associations, the biology of this key transporter is only beginning to be uncovered. Here, we show that systemic overexpression of AT‐1 in the mouse leads to a segmental form of progeria with dysmorphism and metabolic alterations. The phenotype includes delayed growth, short lifespan, alopecia, skin lesions, rectal prolapse, osteoporosis, cardiomegaly, muscle atrophy, reduced fertility, and anemia. In terms of homeostasis, the AT‐1 overexpressing mouse displays hypocholesterolemia, altered glycemia, and increased indices of systemic inflammation. Mechanistically, the phenotype is caused by a block in Atg9a‐Fam134b‐LC3β and Atg9a‐Sec62‐LC3β interactions, and defective reticulophagy, the autophagic recycling of the ER. Inhibition of ATase1/ATase2 acetyltransferase enzymes downstream of AT‐1 restores reticulophagy and rescues the phenotype of the animals. These data suggest that inappropriately elevated acetyl‐CoA flux into the ER directly induces defects in autophagy and recycling of subcellular structures and that this diversion of acetyl‐CoA from cytosol to ER is causal in the progeria phenotype. Collectively, these data establish the cytosol‐to‐ER flux of acetyl‐CoA as a novel event that dictates the pace of aging phenotypes and identify intracellular acetyl‐CoA‐dependent homeostatic mechanisms linked to metabolism and inflammation. 相似文献
993.
994.
Thomas L. Parchman Joshua P. Jahner Kathryn A. Uckele Lanie M. Galland Andrew J. Eckert 《Tree Genetics & Genomes》2018,14(3):39
As tree species vary extensively in genome size, complexity, and resource development, reduced representation methods have been increasingly employed for the generation of population genomic data. By allowing rapid marker discovery and genotyping for thousands of genomic regions in many individuals without requiring genomic resources, restriction site-associated DNA sequencing (RADseq) methods have dramatically improved our ability to bring population genomic perspectives to non-model trees. The rapid recent increase in studies of trees utilizing RADseq suggests that it is likely to become among the most common approaches for generating genome-wide data for a variety of applications. Here we provide a practical review of RADseq and its application to research areas of tree genetics. We briefly review RADseq laboratory methods and consider analytical approaches for assembly, variant calling, and bioinformatic processing. To guide considerations for study design, we use in silico analyses of eight available tree genomes to illustrate how expected marker number and density vary across laboratory approaches and genome sizes, and to consider the ability of RADseq designs to query coding regions. We review the empirical use of RADseq for different research objectives, considering its strengths and limitations. Many studies have used RADseq data to perform genome scans for selection, although limited marker density and linkage disequilibrium will often compromise its utility for such analyses. Regardless of this limitation, RADseq offers a powerful and inexpensive technique for generating genome-wide SNP data that can greatly contribute to research spanning phylogenetic and population genetic inference, linkage mapping, and quantitative genetic parameter estimation for tree genetics. 相似文献
995.
Short‐ and Long‐Term Changes in Health‐Related Quality of Life with Weight Loss: Results from a Randomized Controlled Trial 下载免费PDF全文
996.
Laura Bozal-Basterra Itziar Martín-Ruíz Lucia Pirone Yinwen Liang Jón Otti Sigurðsson Maria Gonzalez-Santamarta Immacolata Giordano Estibaliz Gabicagogeascoa Angela de Luca Jose A. Rodríguez Andrew O.M. Wilkie Jürgen Kohlhase Deborah Eastwood Christopher Yale Jesper V. Olsen Michael Rauchman Kathryn V. Anderson James D. Sutherland Rosa Barrio 《American journal of human genetics》2018,102(2):249-265
997.
Yoshiro Suzuki David Chitayat Hirotake Sawada Matthew A. Deardorff Heather M. McLaughlin Amber Begtrup Kathryn Millar Jennifer Harrington Karen Chong Maian Roifman Katheryn Grand Makoto Tominaga Fumio Takada Shirley Shuster Megumi Obara Hiroshi Mutoh Reiko Kushima Gen Nishimura 《American journal of human genetics》2018,102(6):1104-1114
998.
Kathryn A. Buckley David A. Crook Richard D. Pillans Liam Smith Peter M. Kyne 《Reviews in Fish Biology and Fisheries》2018,28(1):137-151
Zoos and public aquaria exhibit numerous threatened species globally, and in the modern context of these institutions as conservation hubs, it is crucial that displays are ecologically sustainable. Elasmobranchs (sharks and rays) are of particular conservation concern and a higher proportion of threatened species are exhibited than any other assessed vertebrate group. Many of these lack sustainable captive populations, so comprehensive assessments of sustainability may be needed to support the management of future harvests and safeguard wild populations. We propose an approach to identify species that require an assessment of sustainability. Species at risk of extinction in the wild were considered to be those assessed as threatened (CR, EN or VU) on the IUCN Red List of Threatened Species, or data deficient species that may be at an elevated risk of extinction due to life history traits and habitat associations. We defined sustainable captive populations as self-maintaining, or from a source population that can sustain harvest levels without risk of population declines below sustainable levels. The captive breeding and wild harvest records of at risk species displayed by Australian aquaria were examined as a case study. Two species, largetooth sawfish Pristis pristis and grey nurse shark Carcharias taurus, were found to have unsustainable captive populations and were identified as high priorities for comprehensive sustainability assessments. This review highlights the need for changes in permitting practices and zoo and aquarium record management systems to improve conservation outcomes for captive elasmobranchs. 相似文献
999.
Sujay S. Kaushal Arthur J. Gold Susana Bernal Tammy A. Newcomer Johnson Kelly Addy Amy Burgin Douglas A. Burns Ashley A. Coble Eran Hood YueHan Lu Paul Mayer Elizabeth C. Minor Andrew W. Schroth Philippe Vidon Henry Wilson Marguerite A. Xenopoulos Thomas Doody Joseph G. Galella Phillip Goodling Katherine Haviland Shahan Haq Barret Wessel Kelsey L. Wood Norbert Jaworski Kenneth T. Belt 《Biogeochemistry》2018,141(3):281-305
In the Anthropocene, watershed chemical transport is increasingly dominated by novel combinations of elements, which are hydrologically linked together as ‘chemical cocktails.’ Chemical cocktails are novel because human activities greatly enhance elemental concentrations and their probability for biogeochemical interactions and shared transport along hydrologic flowpaths. A new chemical cocktail approach advances our ability to: trace contaminant mixtures in watersheds, develop chemical proxies with high-resolution sensor data, and manage multiple water quality problems. We explore the following questions: (1) Can we classify elemental transport in watersheds as chemical cocktails using a new approach? (2) What is the role of climate and land use in enhancing the formation and transport of chemical cocktails in watersheds? To address these questions, we first analyze trends in concentrations of carbon, nutrients, metals, and salts in fresh waters over 100 years. Next, we explore how climate and land use enhance the probability of formation of chemical cocktails of carbon, nutrients, metals, and salts. Ultimately, we classify transport of chemical cocktails based on solubility, mobility, reactivity, and dominant phases: (1) sieved chemical cocktails (e.g., particulate forms of nutrients, metals and organic matter); (2) filtered chemical cocktails (e.g., dissolved organic matter and associated metal complexes); (3) chromatographic chemical cocktails (e.g., ions eluted from soil exchange sites); and (4) reactive chemical cocktails (e.g., limiting nutrients and redox sensitive elements). Typically, contaminants are regulated and managed one element at a time, even though combinations of elements interact to influence many water quality problems such as toxicity to life, eutrophication, infrastructure corrosion, and water treatment. A chemical cocktail approach significantly expands evaluations of water quality signatures and impacts beyond single elements to mixtures. High-frequency sensor data (pH, specific conductance, turbidity, etc.) can serve as proxies for chemical cocktails and improve real-time analyses of water quality violations, identify regulatory needs, and track water quality recovery following storms and extreme climate events. Ultimately, a watershed chemical cocktail approach is necessary for effectively co-managing groups of contaminants and provides a more holistic approach for studying, monitoring, and managing water quality in the Anthropocene. 相似文献
1000.