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51.
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Mice of the PL/J strain exhibit a high percentage of morphologically abnormal sperm and provide a model for studying the function of abnormal sperm. The ability of such sperm to reach the site of fertilization within the female reproductive tract has been investigated. We have found a decrease in the percentage of structurally abnormal sperm within the population that reaches the oviduct. This observation suggests either that there is an active selection against abnormal sperm or that they are physiologically disadvantaged in reaching the site of fertilization. 相似文献
53.
Anne Grüneberg 《BMJ (Clinical research ed.)》1984,288(6432):1763-1764
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cAMP/theophylline exaggerates cell shape—whether the fibroblastic morphology of controls or the epithelioid shape of colchicine-treated cells. The ultrastructural basis is that cAMP/theophylline increases the number and linearity of microtubules and microfilament bundles, although where also treated with colchicine, the cells adopt a well-spread shape maintained by microfilament bundles alone. Since interference reflection microscopy shows that colchicine promotes the marked alignment of focal contacts (which terminate microfilament bundles) it is concluded that microtubules encourage angular cell form and modify the pattern of adhesions by influencing the directionality of microfilament bundle formation although they are inessential for the maintenance of the spread form or adhesion per se. 相似文献
56.
Cysteine metabolism has been thought to be important to the phenomenon of dimorphism inHistoplasma capsulatum. We sought mutants with genetic blocks in the metabolism of cysteine by selection of colonies resistant to the toxic analogue, selenocystine. The 22 resistant strains thus obtained were all deficient in uptake of cystine from the surrounding medium but were normally able to convert from mycelium to yeast and back again. Furthermore, they had normal quantities of NADH-dependent cystine reductase when this enzyme was measured. We conclude that mutants defective in cystine uptake can be readily obtained by selection of colonies resistant to selenocystine, and that a lesion in cystine-uptake does not appear to affect the phenomenon of dimorphism in this organism.Preliminary reports of this work were presented at the Second International Congress of Mycology, Tampa, 1977 and at the first International Conference on Histoplasmosis, Atlanta, 1978. 相似文献
57.
58.
Anne Dell Howard R. Morris 《Biochemical and biophysical research communications》1982,106(4):1456-1462
Using a combination of Fast Atom Bombardment lonisation and High Field Magnet — Mass Spectrometry we demonstrate here the ability to observe, for the first time, the accurate molecular weights of polypeptides or other important bioorganic substances up to 6000 Dalton in size. This claim is substantiated with data on intact unreduced Insulin and on the intact sweet protein Monellin, which consists of three polypeptide chains. 相似文献
59.
Anne T. Truesdale David A. Johnson Hendrick G. Bedigian Henry C. Outzen George A. Carlson 《Cellular immunology》1982,74(1):120-125
C57BL/6J-bgJ mice are reported to be less susceptible to tumor induction by threshold doses of Moloney murine sarcoma virus than their +/bg littermates, and there are no significant differences between and +/bg mice in which tumors were induced with respect to tumor latency, size, and regression rate. The difference in tumor frequency cannot be accounted for by M-MSV boosting of activity in mice or by depression of activity in +/bg animals. 相似文献
60.
Biogeographic comparisons of marine algal polyphenolics: evidence against a latitudinal trend 总被引:5,自引:1,他引:4
Nancy M. Targett Loren D. Coen Anne A. Boettcher Christopher E. Tanner 《Oecologia》1992,89(4):464-470
Summary Marine allelochemicals generally are present in greater quantity and diversity in tropical than in temperate regions. Marine algal polyphenolics have been reported as an apparent exception to this biogeographic trend, with literature values for phenolic concentrations significantly higher in temperate than in tropical brown algae. In contrast, our results, the first reported for Caribbean brown algae (orders Dictyotales and Fucales), show that many species have high phenolic levels. In addition, both our study and previous studies with north temperate and tropical species demonstrate that there is marked variation in algal phenolic levels within species from different locations. We conclude that high phenolic concentrations occur in species from both temperate and tropical regions, indicating that latitude alone is not a reasonable predictor of plant phenolic concentrations. 相似文献