全文获取类型
收费全文 | 6882篇 |
免费 | 663篇 |
国内免费 | 3篇 |
专业分类
7548篇 |
出版年
2023年 | 44篇 |
2022年 | 122篇 |
2021年 | 234篇 |
2020年 | 97篇 |
2019年 | 136篇 |
2018年 | 152篇 |
2017年 | 112篇 |
2016年 | 227篇 |
2015年 | 395篇 |
2014年 | 360篇 |
2013年 | 436篇 |
2012年 | 611篇 |
2011年 | 542篇 |
2010年 | 350篇 |
2009年 | 261篇 |
2008年 | 390篇 |
2007年 | 396篇 |
2006年 | 345篇 |
2005年 | 313篇 |
2004年 | 324篇 |
2003年 | 269篇 |
2002年 | 251篇 |
2001年 | 79篇 |
2000年 | 60篇 |
1999年 | 71篇 |
1998年 | 73篇 |
1997年 | 41篇 |
1996年 | 43篇 |
1995年 | 35篇 |
1994年 | 43篇 |
1993年 | 41篇 |
1992年 | 32篇 |
1991年 | 36篇 |
1990年 | 34篇 |
1989年 | 30篇 |
1988年 | 24篇 |
1987年 | 27篇 |
1986年 | 23篇 |
1985年 | 30篇 |
1984年 | 38篇 |
1983年 | 23篇 |
1982年 | 29篇 |
1980年 | 21篇 |
1979年 | 25篇 |
1978年 | 29篇 |
1977年 | 32篇 |
1976年 | 25篇 |
1975年 | 22篇 |
1974年 | 23篇 |
1973年 | 20篇 |
排序方式: 共有7548条查询结果,搜索用时 0 毫秒
51.
In the mammalian central nervous system, the majority of fast excitatory synaptic transmission is mediated by glutamate acting on AMPA-type ionotropic glutamate receptors. The abundance of AMPA receptors at the synapse can be modulated through receptor trafficking, which dynamically regulates many fundamental brain functions, including learning and memory. Reversible posttranslational modifications, including phosphorylation, palmitoylation and ubiquitination of AMPA receptor subunits are important regulatory mechanisms for controlling synaptic AMPA receptor expression and function. In this review, we highlight recent advances in the study of AMPA receptor posttranslational modifications and discuss how these modifications regulate AMPA receptor trafficking and function at synapses. 相似文献
52.
James Skelton Michelle A. Jusino Paige S. Carlson Katherine Smith Mark T. Banik Daniel L. Lindner Jonathan M. Palmer Jiri Hulcr 《Molecular ecology》2019,28(22):4971-4986
A prevailing paradigm in forest ecology is that wood‐boring beetles facilitate wood decay and carbon cycling, but empirical tests have yielded mixed results. We experimentally determined the effects of wood borers on fungal community assembly and wood decay within pine trunks in the southeastern United States. Pine trunks were made either beetle‐accessible or inaccessible. Fungal communities were compared using culturing and high‐throughput amplicon sequencing (HTAS) of DNA and RNA. Prior to beetle infestation, living pines had diverse fungal endophyte communities. Endophytes were displaced by beetle‐associated fungi in beetle‐accessible trees, whereas some endophytes persisted as saprotrophs in beetle‐excluded trees. Beetles increased fungal diversity several fold. Over forty taxa of Ascomycota were significantly associated with beetles, but beetles were not consistently associated with any known wood‐decaying fungi. Instead, increasing ambrosia beetle infestations caused reduced decay, consistent with previous in vitro experiments that showed beetle‐associated fungi reduce decay rates by competing with decay fungi. No effect of bark‐inhabiting beetles on decay was detected. Platypodines carried significantly more fungal taxa than scolytines. Molecular results were validated by synthetic and biological mock communities and were consistent across methodologies. RNA sequencing confirmed that beetle‐associated fungi were biologically active in the wood. Metabarcode sequencing of the LSU/28S marker recovered important fungal symbionts that were missed by ITS2, though community‐level effects were similar between markers. In contrast to the current paradigm, our results indicate ambrosia beetles introduce diverse fungal communities that do not extensively decay wood, but instead reduce decay rates by competing with wood decay fungi. 相似文献
53.
Rømer Thomsen K Lou HC Joensson M Hyam JA Holland P Parsons CE Young KS Møller A Stein A Green AL Kringelbach ML Aziz TZ 《PloS one》2011,6(4):e18686
Emotion and reward have been proposed to be closely linked to conscious experience, but empirical data are lacking. The anterior cingulate cortex (ACC) plays a central role in the hedonic dimension of conscious experience; thus potentially a key region in interactions between emotion and consciousness. Here we tested the impact of emotion on conscious experience, and directly investigated the role of the ACC. We used a masked paradigm that measures conscious reportability in terms of subjective confidence and objective accuracy in identifying the briefly presented stimulus in a forced-choice test. By manipulating the emotional valence (positive, neutral, negative) and the presentation time (16 ms, 32 ms, 80 ms) we measured the impact of these variables on conscious and subliminal (i.e. below threshold) processing. First, we tested normal participants using face and word stimuli. Results showed that participants were more confident and accurate when consciously seeing happy versus sad/neutral faces and words. When stimuli were presented subliminally, we found no effect of emotion. To investigate the neural basis of this impact of emotion, we recorded local field potentials (LFPs) directly in the ACC in a chronic pain patient. Behavioural findings were replicated: the patient was more confident and accurate when (consciously) seeing happy versus sad faces, while no effect was seen in subliminal trials. Mirroring behavioural findings, we found significant differences in the LFPs after around 500 ms (lasting 30 ms) in conscious trials between happy and sad faces, while no effect was found in subliminal trials. We thus demonstrate a striking impact of emotion on conscious experience, with positive emotional stimuli enhancing conscious reportability. In line with previous studies, the data indicate a key role of the ACC, but goes beyond earlier work by providing the first direct evidence of interaction between emotion and conscious experience in the human ACC. 相似文献
54.
Edward P. Glenn Alfredo R. Huete Pamela L. Nagler Katherine K. Hirschboeck Paul Brown 《植物科学评论》2007,26(3):139-168
Evapotranspiraton (ET) is the second largest term in the terrestrial water budget after precipitation, and ET is expected to increase with global warming. ET studies are relevant to the plant sciences because over 80% of terrestrial ET is due to transpiration by plants. Remote sensing is the only feasible means for projecting ET over large landscape units. In the past decade or so, new ground and remote sensing tools have dramatically increased our ability to measure ET at the plot scale and to scale it over larger regions. Moisture flux towers and micrometeorological stations have been deployed in numerous natural and agricultural biomes and provide continuous measurements of actual ET or potential ET with an accuracy or uncertainty of 10–30%. These measurements can be scaled to larger landscape units using remotely-sensed vegetation indices (VIs), Land Surface Temperature (LST), and other satellite data. Two types of methods have been developed. Empirical methods use time-series VIs and micrometeorological data to project ET measured on the ground to larger landscape units. Physically-based methods use remote sensing data to determine the components of the surface energy balance, including latent heat flux, which determines ET. Errors in predicting ET by both types of methods are within the error bounds of the flux towers by which they are calibrated or validated. However, the error bounds need to be reduced to 10% or less for applications that require precise wide-area ET estimates. The high fidelity between ET and VIs over agricultural fields and natural ecosystems where precise ground estimates of ET are available suggests that this might be an achievable goal if ground methods for measuring ET continue to improve. 相似文献
55.
Anders Bjrn Katherine Richardson Michael Zwicky Hauschild 《Journal of Industrial Ecology》2019,23(4):838-854
An absolute environmental sustainability assessment (AESA) addresses whether a production or consumption activity can be considered environmentally sustainable in an absolute sense. This involves a comparison of its environmental pressure to its allocated environmental carrying capacity. AESA methods have been developed in multiple academic fields, each using their own set of concepts and terms with little communication across the fields. A recent growing interest in using AESA methods for decision support calls for a better common understanding of the constituents of an AESA method and how it can be communicated to scientific peers and to potential users. With this aim, we develop a framework for AESA methods, composed of a succession of four assessment steps and involving six methodological choices that must be made by the method developer or the user. We then use the framework to analyze and compare five selected AESA methods that focus on the release of phosphorus and nitrogen to the environment. In this manner, we show that the framework is able to systematically differentiate AESA methods that initially appear to be similar. Intended users of the framework include (1) method developers communicating new AESA methods to academic peers or potential method users and (2) researchers comparing a group of existing AESA methods and communicating their differences to their peers and to potential users looking for guidance on method selection. 相似文献
56.
57.
Assessment of two flexible and compatible SNP genotyping platforms: TaqMan SNP Genotyping Assays and the SNPlex Genotyping System 总被引:3,自引:0,他引:3
In this review we describe the principles, protocols, and applications of two commercially available SNP genotyping platforms, the TaqMan SNP Genotyping Assays and the SNPlex Genotyping System. Combined, these two technologies meet the requirements of multiple SNP applications in genetics research and pharmacogenetics. We also describe a set of SNP selection tools and validated assay resources which we developed to accelerate the cycle of experimentation on these platforms. Criteria for selecting the more appropriate of these two genotyping technologies are presented: the genetic architecture of the trait of interest, the throughput required, and the number of SNPs and samples needed for a successful study. Overall, the TaqMan assay format is suitable for low- to mid-throughput applications in which a high assay conversion rate, simple assay workflow, and low cost of automation are desirable. The SNPlex Genotyping System, on the other hand, is well suited for SNP applications in which throughput and cost-efficiency are essential, e.g., applications requiring either the testing of large numbers of SNPs and samples, or the flexibility to select various SNP subsets. 相似文献
58.
59.
60.