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31.
32.
Cells containing immunoreactive vimentin-type intermediate filaments (IF) were identified in paraffin sections and whole-mount preparations of the gerbil inner ear. Most connective tissue cells showed positive immunostaining, although one unusual class of stromal cell lacked vimentin. Several different types of epithelial cells contained high levels of vimentin. In the cochlea, Deiters' cells, inner phalangeal cells, Boettcher's cells, some outer sulcus cells, and the intermediate cells of the stria vascularis showed strong immunoreactivity. Strial basal cells exhibited weaker and less consistent staining. Neither inner nor outer hair cells were stained. In the vestibular system, hair cells with a morphology and location more characteristic of type I than of type II cells showed strong immunostaining for vimentin. Supporting cells in vestibular neurosensory epithelium stained with less intensity. These results were surprising because epithelial cells in vivo only rarely express vimentin-type IF. Although the functional significance of vimentin remains to be established, its presence in some but not other highly specialized cell types provides an excellent marker for investigating the lineage and morphogenesis of the complex inner ear tissues. 相似文献
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Maarten Jaap Erik Broekman Selwyn Hoeks Rosa Freriks Merel M. Langendoen Katharina M. Runge Ecaterina Savenco Ruben ter Harmsel Mark A. J. Huijbregts Marlee A. Tucker 《Global Ecology and Biogeography》2023,32(2):198-205
Motivation
Home range is a common measure of use of space by animals because it provides ecological information that is useful for conservation applications. In macroecological studies, values are typically aggregated to species means to examine general patterns of use of space by animals. However, this ignores the environmental context in which the home range was estimated and does not account for intraspecific variation in home range size. In addition, the focus of macroecological studies on home ranges has historically been biased towards terrestrial mammals. The use of aggregated numbers and the terrestrial focus limit our ability to examine home-range patterns across different environments, their variation in time and variation between different levels of organization. Here, we introduce HomeRange, a global database with 75,611 home-range values across 960 different species of mammals, including terrestrial, aquatic and aerial species.Main types of variables contained
The dataset contains estimates of home ranges of mammals, species names, methodological information on data collection, method of home-range estimation, period of data collection, study coordinates and name of location, in addition to species traits derived from the studies, such as body mass, life stage, reproductive status and locomotor habit.Spatial location and grain
The collected data are distributed globally. Across studies, the spatial accuracy varies, with the coarsest resolution being 1°.Time period and grain
The data represent information published between 1939 and 2022. Across studies, the temporal accuracy varies; some studies report start and end dates specific to the day, whereas for other studies only the month or year is reported.Major taxa and level of measurement
Mammalian species from 24 of the 27 different taxonomic orders. Home-range estimates range from individual-level values to population-level averages.Software format
Data are supplied as a comma-delimited text file (.csv) and can be loaded directly into R using the “HomeRange” R package ( https://github.com/SHoeks/HomeRange ). 相似文献34.
Oxygen uptake measurements have shown that pressurized gas transport, resulting from the physical effect of thermo-osmosis
of gases, improves oxygen supply to the roots of the seedlings in two alder speciesAlnus japonica (Thunb.) Steud. andAlnus hirsuta (Spach) Rupr., which are both native in Japan. When gas transport conditions were established by irradiation of the tree
stems the internal aeration was increased to a level nearly equal to the oxygen demand of the root system in leafless seedlings
ofA. hirsuta, but was higher inA. japonica so that excess oxygen was excreted into the environment. An increase of superoxide dismutase (SOD) activity, which protects
plants from toxic oxygen radicals and post-anoxic injury, has been observed in root tissues ofA. japonica when the seedlings were flooded for 3 days. The increase of SOD activity, in concert with high gas transport rates, may enable
this tree species to grow in wet sites characterized by low oxygen partial pressure in the soil and by varying water tables.
A less effective gas transport, flood-induced reduction of SOD activity in root tissues, and reduced height growth in waterlogged
soil may be responsible for the fact thatA. hirsuta is unable to inhabit wettland sites. 相似文献
35.
Newly hatched young of the benthic, coastal-living octopod, Octopus vulgaris , enter the plankton and remain there for perhaps eight weeks. At hatching the arms are short and bear a few, large, primary suckers. The buccal mass is relatively large in proportion to the size of the animal. The eyes are large. The central nervous system has fairly well-defined lobes, some of which develop earlier than others. We shall follow the development of several features of O. vulgaris from hatching, through its life in the plankton until settlement and correlate them with changes in the brain and behaviour. 相似文献
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Selection of cell lines resistant to anti-transferrin receptor antibody: evidence for a mutation in transferrin receptor. 总被引:6,自引:1,他引:5
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Some anti-murine transferrin receptor monoclonal antibodies block iron uptake in mouse cell lines and inhibit cell growth. We report here the selection and characterization of mutant murine lymphoma cell lines which escape this growth inhibition by anti-transferrin receptor antibody. Growth assays and immunoprecipitation of transferrin receptor in hybrids between independently derived mutants or between mutants and antibody-susceptible parental cell lines indicate that all of the selected lines have a similar genetic alteration that is codominantly expressed in hybrids. Anti-transferrin receptor antibodies and transferrin itself still bind to the mutant lines with saturating levels and Kd values very similar to those of the parental lines. However, reciprocal clearing experiments by immunoprecipitation and reciprocal blocking of binding to the cell surface with two anti-transferrin receptor antibodies indicate that the mutant lines have altered a fraction of their transferrin receptors such that the growth-inhibiting antibody no longer binds, whereas another portion of their transferrin receptors is similar to those of the parental lines and binds both antibodies. These results argue that the antibody-selected mutant cell lines are heterozygous in transferrin receptor expression, probably with a mutation in one of the transferrin receptor structural genes. 相似文献
39.
Summary Crypts of the lingual tonsil were investigated in 10 male and female Macaca fascicularis by use of correlated light and scanning-electron microscopy. Counting of crypt openings provided an estimate of the total number of respective crypto-lymphatic units, which were found to range from 20 to 39. Crypt openings appeared in three distinct morphological varieties, i.e. circular, oval or slit-like. Tonsillar units existed individually or were arranged in a rosary fashion below a slit-like mucosal fold serving as a common exit. Although the crypt epithelium was generally non-keratinized, individual cells showing a surface pattern similar to that of the keratinized cells could be encountered. The crypt epithelium was frequently fragmented and showed heavy mononuclear cell infiltration and surface discontinuities, with lymphoid cells coming in contact with luminal contents. The crypt lumen either appeared as a simple epithelial invagination or existed as a complex, cavernous pouch with many blind-ending diverticula. The lumen contained a mixture of exfoliated epithelial cells, leucocytes and bacteria. The secretory ducts of the posterior lingual glands opened occasionally at various levels into the crypt lumina or independently to the exterior. 相似文献
40.
A total of 19 different species belonging to the genera Asperula, Galium, Rubia and Sherardia were taken into cell culture. All species, differentiated plants as well as tissue cultures, produced anthraquinones in differing yields. Cells were grown in a basal medium containing 7 differently substituted phenoxyacetic acids, as well as 1-naphthaleneacetic acid, all at 10–5 M concentration. The effectors supporting highest pigment production in each culture were selected and, in the presence of the selected effector, the sucrose content of the medium was then varied from 1 to 14%. Anthraquinone formation was thus optimized for each individual species, but no general pattern, either of effector quality or of sucrose concentration, emerged. In 17 out of 19 cases secondary product formation in optimized cell cultures surpassed that of differentiated plants. The highest anthraquinone yield was observed with Galium verum (1.7 g/l) and the highest concentration achieved with Rubia fruticosa (20% of dry weight).Abbreviations 2,4-D
2,4-dichlorophenoxyacetic acid
- NAA
1-naphthaleneacetic acid
- PAA
phenoxyacetic acid
- dwt
dry weight 相似文献