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A total of 187 Patients with suspected onychomycosis were examined for causative fungal agents between 1996 and 1997. Laboratory
examination confirmed onychomycosis in 115 patients, of which 97 cases were presented with positive microscopic and cultural
examinations, and they were selected for itraconazole pulse therapy. From an etiological point of view, 48.4% of the nail
infections, mainly toenail infections, were caused by dermatophytes, 43.3% were infected with Candida spp, specially infected fingernails, and 8.2% by non-dermatophytic molds. Trichophyton mentagrophytes var. interdigital and T. violaceum were the most prevalent species. Candida albicans and C. parapsilosis were the predominant species of the Genus Candida. Scopolariopsis brevicaulis was the most common non-dermatophyte molds observed. Female affected more frequently than male and in both sexes, those who
were 30–49 years old, more infected. Toenails were affected more frequently than fingernails. In this study, itraconazole
pulse therapy (400 mg daily) gave during the first week of per month for 3 months. The study included 51 patients with toenail
onychomychosis (group 1) and 46 patients with fingernail infections (group 2). Patients were followed up for 9 months after
the last treatment. Clinical response rates were 83% in the group 1, 95% in the group 2 at month 12; the corresponding mycological
cure rates were 71 and 87%, respectively.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
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Rashidbaghan Azam Mostafaie Ali Yazdani Yaghoub Mansouri Kamran 《Biochemical genetics》2021,59(4):1049-1064
Biochemical Genetics - Treatment of acute myeloid leukemia (AML) requires new drugs as result of a rise in new cases and high disease relapse. Plant lectins with the ability to bind carbohydrates... 相似文献
88.
Kashanian S Khodaei MM Roshanfekr H Shahabadi N Rezvani A Mansouri G 《DNA and cell biology》2011,30(5):287-296
The DNA binding behavior of [Cu(phen)(phen-dione)Cl]Cl (1) and [Cu(bpy)(phen-dione)Cl]Cl (2) was studied with a series of techniques including UV-vis absorption, circular dichroism spectroscopy, and viscometric methods. Cytotoxicity effect and DNA unwinding properties were also investigated. The results indicate that the Cu(II) complexes interact with calf-thymus DNA by both partially intercalative and hydrogen binding. These findings have been further substantiated by the determination of intrinsic binding constants spectrophotometrically, 12.5?×?10(5) and 5?×?10(5) for 1 and 2, respectively. Our findings suggest that the type of ligands and structure of complexes have marked effect on the binding affinity of complexes involving CT-DNA. Circular dichroism results show that complex 1 causes considerable increase in base stacking of DNA, whereas 2 decreases the base stacking, which is related to more extended aromatic area of 1,10-phenanthroline in 1 rather than bipyridine in 2. Slow decrease in DNA viscosity indicates partially intercalative binding in addition to hydrogen binding on the surface of DNA. The second binding mode was also confirmed by additional tests: interaction in denaturation condition and acidic pH. Also, these new complexes induced cleavage in pUC18 plasmid DNA as indicated in gel electrophoresis and showed excellent antitumor activity against K562 (human chronic myeloid leukemia) cells. 相似文献
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Interferon-induced BST2/Tetherin prevents budding of vpu-deficient HIV-1 by tethering mature viral particles to the plasma membrane. BST2 also inhibits release of other enveloped viruses including Ebola virus and Kaposi's sarcoma associated herpesvirus (KSHV), indicating that BST2 is a broadly acting antiviral host protein. Unexpectedly however, recovery of human cytomegalovirus (HCMV) from supernatants of BST2-expressing human fibroblasts was increased rather than decreased. Furthermore, BST2 seemed to enhance viral entry into cells since more virion proteins were released into BST2-expressing cells and subsequent viral gene expression was elevated. A significant increase in viral entry was also observed upon induction of endogenous BST2 during differentiation of the pro-monocytic cell line THP-1. Moreover, treatment of primary human monocytes with siRNA to BST2 reduced HCMV infection, suggesting that BST2 facilitates entry of HCMV into cells expressing high levels of BST2 either constitutively or in response to exogenous stimuli. Since BST2 is present in HCMV particles we propose that HCMV entry is enhanced via a reverse-tethering mechanism with BST2 in the viral envelope interacting with BST2 in the target cell membrane. Our data suggest that HCMV not only counteracts the well-established function of BST2 as inhibitor of viral egress but also employs this anti-viral protein to gain entry into BST2-expressing hematopoietic cells, a process that might play a role in hematogenous dissemination of HCMV. 相似文献
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In this study the influence of gibberellic acid (GA3) on plastidic and cytosolic terpenoids and on two key enzymes, 1-deoxy-d-xylulose-5-phosphate synthase (DXS) and 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR), for terpenoid biosynthesis
was compared in vegetative cannabis plants. Treatment with GA3 resulted in a decrease of DXS activity in comparison with the control plants. The amount of chlorophylls a, b and total carotenoids declined when plants treated by GA3 in a concentration dependent manner. The α-tocopherol content of cannabis plants decreased in 50 μM GA3 treatment and increased in 100 μM GA3 treatment. Exogenous GA3 caused an increase in HMGR activity. Concomitant with this result, the amount of squalene and phytosterols increased with
GA3 treatment. The amount of THC and CBD did not change at 50 μM GA3 treatment, but applying of 100 μM GA3 increased THC and CBD content in leaf plant in comparison with control plants. GA3 treatment declined number and percentage of monoterpenes in treated plants. Also the number of sesquiterpenes decreased in
response to GA3 treatment but among the remainder of them, the amount of some sesquiterpenes decreased and some sesquiterpenes increased
with GA3 treatment. Our results showed that GA3 treatment had opposite effect on primary terpenoid biosynthesis by the plastidic 2C-methyl-d-erythritol 4-phosphate (MEP) and mevalonate (MVA) pathways. But secondary terpenoids showed different response to GA3 treatment probably due to interference of two biosynthetic pathways in their formation. 相似文献