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Production and characterization of a monoclonal antibody able to discriminate galectin-1 from galectin-2 and galectin-3 总被引:1,自引:0,他引:1
Cornillot JD; Pontet M; Dupuy C; Chadli A; Caron M; Joubert-Caron R; Bourin P; Bladier D 《Glycobiology》1998,8(5):425-432
Antisera raised against galectin-1 exhibit crossreactivities with other
galectins or related molecules. In order to overcome this problem, a
monoclonal antibody to human brain galectin-1 was obtained by selecting
clones without reactivity toward galectin-3. This mAb specifically bound
galectin-1 of various animal origins but neither galectin-2 nor galectin-3.
Western-blotting analysis of soluble human brain extracts after 2D gel
electrophoresis revealed only the two most acidic isoforms of galectin-1.
The ability of this mAb to bind galectin-1/asialofetuin complexes indicates
that its epitope is not localized in the carbohydrate recognition domain of
galectin-1. This particularity induces with efficiency its monospecificity.
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An anchored restriction-mapping approach applied to the genetic analysis of the Anopheles gambiae malaria vector complex 1 总被引:1,自引:0,他引:1
We introduce here a simple approach for rapidly determining restriction
maps for a number of regions of a genome; this involves "anchoring" a map
with a rare restriction site (in this case the seldom-cutting EagI)
followed by partial digestion of a frequent-cutting enzyme (e.g., Sau 3A).
We applied this technology to five species of the Anopheles gambiae
complex. In a single Southern blot we obtained about a 15-kb restriction
map each for the mtDNA, rRNA gene, and a scnDNA region for each of five
species. Phylogenetic analyses of these regions yield trees at odds with
the more traditional chromosome inversion-based trees. The value of the
approach for systematic purposes is the ease with which several large,
independent regions of the genome can be quickly assayed for molecular
variation.
相似文献
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Testing lack of fit in multiple regression 总被引:2,自引:0,他引:2
35.
Axel Abelein J?rn D?vling Kaspersen S?ren Bang Nielsen Grethe Vestergaard Jensen Gunna Christiansen Jan Skov Pedersen Jens Danielsson Daniel E. Otzen Astrid Gr?slund 《The Journal of biological chemistry》2013,288(32):23518-23528
Intermediate amyloidogenic states along the amyloid β peptide (Aβ) aggregation pathway have been shown to be linked to neurotoxicity. To shed more light on the different structures that may arise during Aβ aggregation, we here investigate surfactant-induced Aβ aggregation. This process leads to co-aggregates featuring a β-structure motif that is characteristic for mature amyloid-like structures. Surfactants induce secondary structure in Aβ in a concentration-dependent manner, from predominantly random coil at low surfactant concentration, via β-structure to the fully formed α-helical state at high surfactant concentration. The β-rich state is the most aggregation-prone as monitored by thioflavin T fluorescence. Small angle x-ray scattering reveals initial globular structures of surfactant-Aβ co-aggregated oligomers and formation of elongated fibrils during a slow aggregation process. Alongside this slow (minutes to hours time scale) fibrillation process, much faster dynamic exchange (kex ∼1100 s−1) takes place between free and co-aggregate-bound peptide. The two hydrophobic segments of the peptide are directly involved in the chemical exchange and interact with the hydrophobic part of the co-aggregates. Our findings suggest a model for surfactant-induced aggregation where free peptide and surfactant initially co-aggregate to dynamic globular oligomers and eventually form elongated fibrils. When interacting with β-structure promoting substances, such as surfactants, Aβ is kinetically driven toward an aggregation-prone state. 相似文献
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Background
Quantifying the amount of standing genetic variation in fitness represents an empirical challenge. Unfortunately, the shortage of detailed studies of the genetic architecture of fitness has hampered progress in several domains of evolutionary biology. One such area is the study of sexual selection. In particular, the evolution of adaptive female choice by indirect genetic benefits relies on the presence of genetic variation for fitness. Female choice by genetic benefits fall broadly into good genes (additive) models and compatibility (non-additive) models where the strength of selection is dictated by the genetic architecture of fitness. To characterize the genetic architecture of fitness, we employed a quantitative genetic design (the diallel cross) in a population of the seed beetle Callosobruchus maculatus, which is known to exhibit post-copulatory female choice. From reciprocal crosses of inbred lines, we assayed egg production, egg-to-adult survival, and lifetime offspring production of the outbred F1 daughters (F1 productivity). 相似文献38.
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