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SORAYA C M LEAL-BERTIOLI ALETÉIA V PASCOAL PATRÍCIA M GUIMARÃES MARIA F GROSSI DE SÁ REJANE L GUIMARÃES DAMARES C MONTE DAVID J BERTIOLI 《The Annals of applied biology》2003,143(3):349-357
The protein Tarin 1, from Colocasia esculenta, was expressed in Nicotiana tabacum. Bioassays were done on plants expressing Tarin 1 at different levels using Spodoptera frugiperda larvae, various bacteria and fungi and the root‐knot nematode Meloidogyne javanica. It was found that S. frugiperda larvae fed on transformed plants had retarded and lower pupation, lower accumulated biomass and higher mortality rate than larvae fed on control plants. Also, Tarin 1 was found to inhibit the growth in vitro of Pseudomonas syringae pv. tomato. For Meloidogyne javanica, both relative replication and root damage were greater in control plants than in transformed plants, but the results were not statistically significant. This work illustrates the effects of plants expressing Tarin 1, on the growth and development of insects and bacteria, and shows its potential for pest management. 相似文献
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Ermakova IT Starovoĭtov II Tikhonova EB Slepen'kin AV Kashparov KI Boronin AM 《Mikrobiologiia》2002,71(5):604-610
The investigation of the degradation of thiodiglycol (the major product of mustard gas hydrolysis) by Alcaligenes xylosoxydans subsp. denitrificans strain TD2 showed that thiodiglycol is metabolized through the oxidation of its primary alcohol groups and the subsequent cleavage of C-S bonds in the intermediate products, thiodiglycolic and thioglycolic acids. The end products of these reactions are SO4(2-) ions and acetate, the latter being involved in the central metabolism of strain TD2. The oxidation of the sulfur atom gives rise to diglycolsulfoxide, which is recalcitrant to further microbial degradation. Based on the data obtained, a metabolic pathway of thiodiglycol transformation by A. xylosoxydans subsp. denitrificans strain TD2 is proposed. 相似文献
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Chunshan Guo Wei Cui Xue Feng Jianzhou Zhao Guihua Lu Beijing Kaituo DNA Biotech Research Center Beijing China Pioneer Hi-Bred International Inc. a DuPont Company Johnston IA USA The Institute of Genetics Developmental Biology the Chinese Academy of Sciences Beijing China. 《Acta Botanica Sinica》2011,(3)
Sorghum (Sorghum bicolor) has high levels of starch,sugar,and fiber and is one of the most important energy crops in the world.Insect damage is one of the challenges that impacts sorghum biomass production.There are at least 150 insect species that can infest sorghum varieties worldwide.These insects can complete several generations within a growing season,they target various parts of sorghum plants at developmental stages,and they cause significant biomass losses.Genetic research has revealed the existence... 相似文献
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卤虫染色体倍性组成的研究 总被引:8,自引:1,他引:8
本文报道了我国4个地理品系卤虫的染色体倍性组成及卵径分布,分析了卤虫染色体的非整倍性,讨论了卤虫种的分布。 天津卤虫有2倍体(2n=42),占33.3%和5倍体,占16.2%;染色体数的波动为21—108;卵径为262±17μm。 海南卤虫有2倍体(2n=42),占8%;4倍体,占17.7%;5倍体,占18.3%;染色体数的波动为16—111;卵径为285±15μm。 新疆卤虫有2倍体(2n=42),占39.2%;染色体数的波动为17—106;卵径为269±15μm。 山西卤虫有2倍体(2n=42),占45.1%,染色体数的波动为17—84;卵径为234±13μm。 相似文献
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桃儿七光合生理特性的地理差异研究 总被引:7,自引:3,他引:4
对中国由南向北5个种源地桃儿七(Sinopodophyllum hexandrum)的光合生理生态特性进行了研究.结果表明:(1)北部宁夏六盘山地区植株的光饱和点(LSP)和光补偿点(LCP)最高,表观光量子效率(AQY)、瞬时光能利用效率(ILUE)和最大光合速率(Pmax)最低,光合能力最差;最南部云南纳帕海植株的LSP和LCP很低,但因其AQY和ILUE最高,故其Pmax最大,强光下的光合能力最高.(2)最南部云南纳帕海和最北部甘肃兴隆山个体的羧化效率(CE)最高,且CO2补偿点(CCP)较低,因此CO2利用率较高;而较南部四川刷经寺个体的CE最低,且CCP最高,故CO2利用效率最低.(3)各种源植株叶片的叶绿素总量及叶绿素a含量无显著差异,而不同地区个体的Chla/Chlb值差异显著,最北部甘肃兴隆山植株的Chla/Chlb值最大,而南部四川刷经寺的Chla/Chlb值最小.(4)各种源地植株的水分利用效率(WUE)和蒸腾速率(Tr)对光量子通量密度(PFD)与CO2浓度的响应过程也表现出显著的地理分异.总的来看,桃儿七是一种喜光但又较耐阴植物,但不同分布区桃儿七的光合生理生态特征已经出现了较显著的地理分化. 相似文献
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Increased Level of β-Amyloid in the Brain of Bulbectomized Mice 总被引:1,自引:0,他引:1
Aleksandrova IY Kuvichkin VV Kashparov IA Medvinskaya NI Nesterova IV Lunin SM Samokhin AN Bobkova NV 《Biochemistry. Biokhimii?a》2004,69(2):176-180
Six weeks after bilateral olfactory bulbectomy, a peptide with molecular weight of 4 kD was revealed in extracts of the neocortex and hippocampus from mice. Using monoclonal antibodies 4G8, this peptide was identified as beta-amyloid. Its level was significantly higher in the bulbectomized animals than in sham-operated mice. The bulbectomized mice displayed sharp impairment in spatial memory when tested in the Morris water maze. The results suggest that bulbectomy initiates in the brain a pathological process similar to human Alzheimer's disease in location, biochemistry, and behavioral manifestations. 相似文献
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On the basis of theoretical conformational analysis of separate peptide fragments, the conformational characteristics of two substrates and a substrate-like inhibitor of aspartic protease rhizopuspepsin were studied. It was shown that the spatial structure of these molecules is described by several families of conformations, the transition between which does not require the overcoming of high energy barriers. It was assumed that the stabilization of beta-structural conformations experimentally observed in inhibitor complexes is due to the greater predisposition of extended structures to the formation of effective intermolecular contacts with amino acid residues of the active site of the enzyme. 相似文献