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Andryushina V. A. Karpova N. V. Druzhinina A. V. Stytsenko T. S. Podorozhko E. A. Ryabev A. N. Lozinsky V. I. 《Applied Biochemistry and Microbiology》2015,51(5):530-538
Applied Biochemistry and Microbiology - The steroid-transforming activity of free and immobilized cells of Pimelobacter simplex VKPM Ac-1632 entrapped in an operationally stable macroporous... 相似文献
13.
The complex N-AcPhe-tRNA(Phe).poly(U).80 S ribosome from human placenta was treated with puromycin taken in various concentrations. Based on the kinetic data of N-acetylphenylalanyl-puromycin formation, the association constant of puromycin with the acceptor site of the ribosome was estimated to be (3.96 +/- 0.84) x 10(4) M-1 at 37 degrees C. 相似文献
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K J Atabekov L G Tyulkina O V Karpova V G Metelev N P Rodionova Z A Shabarova J G Atabekov 《FEBS letters》1988,232(1):96-98
The TMV RNA molecule can be cleaved at a single site by RNase H directed by chimeric oligo(deoxyribo-ribo)nucleotide with an internucleotide pyrophosphate bond. 相似文献
16.
John S. Choy Eileen O'Toole Breanna M. Schuster Matthew J. Crisp Tatiana S. Karpova James G. McNally Mark Winey Melissa K. Gardner Munira A. Basrai 《Molecular biology of the cell》2013,24(17):2753-2763
How subunit dosage contributes to the assembly and function of multimeric complexes is an important question with implications in understanding biochemical, evolutionary, and disease mechanisms. Toward identifying pathways that are susceptible to decreased gene dosage, we performed a genome-wide screen for haploinsufficient (HI) genes that guard against genome instability in Saccharomyces cerevisiae. This led to the identification of all three genes (SPC97, SPC98, and TUB4) encoding the evolutionarily conserved γ-tubulin small complex (γ-TuSC), which nucleates microtubule assembly. We found that hemizygous γ-TuSC mutants exhibit higher rates of chromosome loss and increases in anaphase spindle length and elongation velocities. Fluorescence microscopy, fluorescence recovery after photobleaching, electron tomography, and model convolution simulation of spc98/+ mutants revealed improper regulation of interpolar (iMT) and kinetochore (kMT) microtubules in anaphase. The underlying cause is likely due to reduced levels of Tub4, as overexpression of TUB4 suppressed the spindle and chromosome segregation defects in spc98/+ mutants. We propose that γ-TuSC is crucial for balanced assembly between iMTs and kMTs for spindle organization and accurate chromosome segregation. Taken together, the results show how gene dosage studies provide critical insights into the assembly and function of multisubunit complexes that may not be revealed by using traditional studies with haploid gene deletion or conditional alleles. 相似文献
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E. V. Bogomolova N. V. Adonyeva E. K. Karpova N. E. Gruntenko I. Yu. Rauschenbach 《Russian Journal of Genetics》2013,49(7):778-781
The effect of the ubiquitous downregulation of insulin receptor (InR) gene expression on the metabolism of juvenile hormone (JH) and dopamine (DA) in young females of D. melanogaster under normal conditions and heat stress is studied. The level of JH degradation and alkaline phosphatase activity (ALP, an enzyme regulating DA synthesis) were used as indicators of JH and DA levels, respectively. We demonstrated that, under normal conditions, the ubiquitous inhibition of the InR gene expression in D. melanogaster females induced an increase in the JH degradation and ALP activity. As we have already shown, this is indicative of the decrease in the concentration of the above hormones. It was also found that the total inactivation of InR does not affect the initiation of JH and DA metabolic system response to heat stress; however, it does affect its intensity. Thus, the involvement of the insulin signaling pathway in the regulation of the JH and DA metabolism in Drosophila females was demonstrated in vivo under normal and stress conditions. 相似文献
18.
V. G. Solovyev A. Sh. Bychevsky I. A. Karpova 《Biochemistry (Moscow) Supplemental Series B: Biomedical Chemistry》2013,7(1):84-89
Estrogen and progestin (ethinylestradiol and levonorgestrel, respectively) in a dose dependent manner accelerated platelet lipid peroxidation (LPO), activated platelets, increased continuous intravascular coagulation and reduced tolerance to thrombin. Antioxidants limited these effects. 相似文献
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Astakhova T. M. Bozhok G. A. Alabedal’karim N. M. Karpova Ya. D. Lyupina Yu. V. Ushakova E. M. Legach E. I. Bondarenko T. P. Sharova N. P. 《Russian Journal of Developmental Biology》2019,50(5):261-267
Russian Journal of Developmental Biology - The aim of this work is to investigate the dynamics for the ovarian tissue engraftment of inbred August rats transplanted to outbred Wistar rats and vice... 相似文献
20.
Vasilyeva A. E. Yanshina D. D. Karpova G. G. Malygin A. A. 《Russian Journal of Bioorganic Chemistry》2019,45(6):758-765
Russian Journal of Bioorganic Chemistry - The ribosomal protein uS15 is one of the key proteins forming the small (40S) ribosomal subunit structure around the central domain of 18S rRNA. According... 相似文献