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151.
Konstantin Tomanov Anja Zeschmann Rebecca Hermkes Karolin Eifler Ionida Ziba Michele Grieco Maria Novatchkova Kay Hofmann Holger Hesse Andreas Bachmair 《The Plant cell》2014,26(11):4547-4560
The Arabidopsis thaliana genes PROTEIN INHIBITOR OF ACTIVATED STAT LIKE1 (PIAL1) and PIAL2 encode proteins with SP-RING domains, which occur in many ligases of the small ubiquitin-related modifier (SUMO) conjugation pathway. We show that PIAL1 and PIAL2 function as SUMO ligases capable of SUMO chain formation and require the SUMO-modified SUMO-conjugating enzyme SCE1 for optimal activity. Mutant analysis indicates a role for PIAL1 and 2 in salt stress and osmotic stress responses, whereas under standard conditions, the mutants show close to normal growth. Mutations in PIAL1 and 2 also lead to altered sulfur metabolism. We propose that, together with SUMO chain binding ubiquitin ligases, these enzymes establish a pathway for proteolytic removal of sumoylation substrates. 相似文献
152.
Salla T. Jaakkola Karolin Zerulla Qinggong Guo Ying Liu Hongling Ma Chunhe Yang Dennis H. Bamford Xiangdong Chen J?rg Soppa Hanna M. Oksanen 《PloS one》2014,9(10)
Live bacteria and archaea have been isolated from several rock salt deposits of up to hundreds of millions of years of age from all around the world. A key factor affecting their longevity is the ability to keep their genomic DNA intact, for which efficient repair mechanisms are needed. Polyploid microbes are known to have an increased resistance towards mutations and DNA damage, and it has been suggested that microbes from deeply buried rock salt would carry several copies of their genomes. Here, cultivable halophilic microbes were isolated from a surface sterilized middle-late Eocene (38–41 million years ago) rock salt sample, drilled from the depth of 800 m at Yunying salt mine, China. Eight unique isolates were obtained, which represented two haloarchaeal genera, Halobacterium and Halolamina. We used real-time PCR to show that our isolates are polyploid, with genome copy numbers of 11–14 genomes per cell in exponential growth phase. The ploidy level was slightly downregulated in stationary growth phase, but the cells still had an average genome copy number of 6–8. The polyploidy of halophilic archaea living in ancient rock salt might be a factor explaining how these organisms are able to overcome the challenge of prolonged survival during their entombment. 相似文献
153.
Sabrina Graf Dominik Schmieden Karolin Tschauner Sabine Hunke Gottfried Unden 《Journal of bacteriology》2014,196(5):1084-1093
The DctSR two-component system of Bacillus subtilis controls the expression of the aerobic C4-dicarboxylate transporter DctA. Deletion of DctA leads to an increased dctA expression. The inactivation of DctB, an extracellular binding protein, is known to inhibit the expression of dctA. Here, interaction between the sensor kinase DctS and the transporter DctA as well as the binding protein DctB was demonstrated in vivo using streptavidin (Strep) or His protein interaction experiments (mSPINE or mHPINE), and the data suggest that DctA and DctB act as cosensors for DctS. The interaction between DctS and DctB was also confirmed by the bacterial two-hybrid system (BACTH). In contrast, no indication was obtained for a direct interaction between the transporter DctA and the binding protein DctB. Activity levels of uptake of [14C]succinate by bacteria that expressed DctA from a plasmid were similar in the absence and the presence of DctB, demonstrating that the binding protein DctB is not required for transport. Thus, DctB is involved not in transport but in cosensing with DctS, highlighting DctB as the first example of a TRAP-type binding protein that acts as a cosensor. The simultaneous presence of DctS/DctB and DctS/DctA sensor pairs and the lack of direct interaction between the cosensors DctA and DctB indicate the formation of a tripartite complex via DctS. It is suggested that the DctS/DctA/DctB complex forms the functional unit for C4-dicarboxylate sensing in B. subtilis. 相似文献
154.
A feed-forward repression mechanism anchors the Sin3/histone deacetylase and N-CoR/SMRT corepressors on chromatin 总被引:3,自引:0,他引:3
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155.
Structure and function of nucleosome assembly proteins. 总被引:1,自引:0,他引:1
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158.
Lutz Kunzmann Christian Müller Karolin Moraweck Dorothea Bräutigam Torsten Wappler André Nel 《Pal?ontologische Zeitschrift》2017,91(3):315-326
The first unequivocal insect remains from compression plant assemblages of the central German Leipzig Embayment (former Weißelster Basin) are reported and briefly described. The fossil taphocoenosis comes from the upper Bartonian or lower Priabonian Luckenau Clay complex (lignite-bearing Borna Formation) that represents sediments of coastal alluvial braidplain origin. Autecological interpretations of insect taxa are in accordance with plant-based environmental reconstructions as three of five insect taxa are closely related to freshwater environments. Based on the accompanying plant assemblage the palaeoclimate of the habitat is characterized as “subtropical” and humid, but with a distinctive seasonality in precipitation. 相似文献
159.
Román González-Prieto Karolin Eifler-Olivi Laura A. Claessens Edwin Willemstein Zhenyu Xiao Cami M.P. Talavera Ormeno Huib Ovaa Helle D. Ulrich Alfred C.O. Vertegaal 《Cell reports》2021,34(4):108691
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160.
Histone Acetylation near the Nucleosome Dyad Axis Enhances Nucleosome Disassembly by RSC and SWI/SNF