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91.
Łukasz Mokros Michał Seweryn Karbownik Katarzyna Nowakowska-Domagała Janusz Szemraj Łukasz Wieteska Karol Woźniak 《Biological Rhythm Research》2016,47(6):865-871
Background: There is barely any evidence of antipsychotic drugs affecting the molecular clockwork in human, yet it is suggested that clock genes are associated with dopaminergic transmission, i.e. the main target of this therapeutics. We decided to verify if haloperidol and olanzapine affect expression of CLOCK, BMAL1, PER1 and CRY1 in a human central nervous system cell line model. Methods: U-87MG human glioblastoma cell line was used as an experimental model. The cells were incubated with or without haloperidol and olanzapine in the concentration of 5 and 20 μM for 24 h. Real-time quantitative polymerase chain reaction with the ΔCT analysis was used to examine the effect of haloperidol and olanzapine on the mRNA expression of the genes. Results: At 5 μM, haloperidol decreased expression of CRY1 almost 20-fold. There was nearly a 1.5-fold increase in expression of PER1. Considering the 20 μM haloperidol concentration and both olanzapine concentrations, no other statistically significant effect was observed. Conclusions: At certain concentration, haloperidol seems to affect expression of particular clock genes in a human central nervous system cell line model, yet mechanism underlying this phenomenon remains elusive. 相似文献
92.
Suppression and synthetic‐lethal genetic relationships of ΔgpsB mutations indicate that GpsB mediates protein phosphorylation and penicillin‐binding protein interactions in Streptococcus pneumoniae D39 下载免费PDF全文
Britta E. Rued Jiaqi J. Zheng Andrea Mura Ho‐Ching T. Tsui Michael J. Boersma Jeffrey L. Mazny Federico Corona Amilcar J. Perez Daniela Fadda Linda Doubravová Karolína Buriánková Pavel Branny Orietta Massidda Malcolm E. Winkler 《Molecular microbiology》2017,103(6):931-957
GpsB regulatory protein and StkP protein kinase have been proposed as molecular switches that balance septal and peripheral (side‐wall like) peptidoglycan (PG) synthesis in Streptococcus pneumoniae (pneumococcus); yet, mechanisms of this switching remain unknown. We report that ΔdivIVA mutations are not epistatic to ΔgpsB division‐protein mutations in progenitor D39 and related genetic backgrounds; nor is GpsB required for StkP localization or FDAA labeling at septal division rings. However, we confirm that reduction of GpsB amount leads to decreased protein phosphorylation by StkP and report that the essentiality of ΔgpsB mutations is suppressed by inactivation of PhpP protein phosphatase, which concomitantly restores protein phosphorylation levels. ΔgpsB mutations are also suppressed by other classes of mutations, including one that eliminates protein phosphorylation and may alter division. Moreover, ΔgpsB mutations are synthetically lethal with Δpbp1a, but not Δpbp2a or Δpbp1b mutations, suggesting GpsB activation of PBP2a activity. Consistent with this result, co‐IP experiments showed that GpsB complexes with EzrA, StkP, PBP2a, PBP2b and MreC in pneumococcal cells. Furthermore, depletion of GpsB prevents PBP2x migration to septal centers. These results support a model in which GpsB negatively regulates peripheral PG synthesis by PBP2b and positively regulates septal ring closure through its interactions with StkP‐PBP2x. 相似文献
93.
First discovery of the charophycean green alga Lychnothamnus barbatus (Charophyceae) extant in the New World 下载免费PDF全文
94.
Dorothea Bedigian Sebsebe Demissew Paul Gepts Daniel F. Austin Neil A. Harriman John Klock Sarah Walshaw John Richard Stepp Beverly J. Brown Julie Polley David Winston Barbara Pickersgill Patrick Van Damme Nina L. Etkin Beronda L. Montgomery Linda Perry Stephen E Siebert Robert J. Krueger Kathleen McConnell Wendy Applequist Mary Theresa Bonhage-Freund Karol Chandler-Ezell 《Economic botany》2005,59(4):395-412
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Daniel F. Austin Richard Felger Karol Chandler-Ezell Michael J. Balick Kurt Allerslev Reynertson Alfredo Gomez-Beloz My Lien T. Nguyen Rob Dean Neil A. Harriman Dorothea Bedigian Mary Theresa Bonhage-Freund Wendy Applequist Linda M. Lyon John Klock My Lien T. Nguyen 《Economic botany》2005,59(2):197-209
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Stacey DH Fox BC Poore SO Bentz ML Gutowski KA 《Plastic and reconstructive surgery》2008,122(4):120e-127e
LEARNING OBJECTIVES:: After studying this article, the participant should be able to: 1. Identify risk factors associated with community-acquired methicillin-resistant Staphylococcus aureus. 2. Recognize the clinical presentation of patients with community-acquired methicillin-resistant S. aureus. 3. Understand the treatment and indications for decolonization of patients who have community-acquired methicillin-resistant S. aureus infections. SUMMARY:: Community-acquired methicillin-resistant Staphylococcus aureus has evolved over the past 10 years as a new health threat seen by plastic surgeons and is an increasing cause of soft-tissue infections. This pathogen has several distinct virulence factors and unique antimicrobial susceptibilities that distinguish methicillin-resistant S. aureus from traditional hospital-acquired methicillin-resistant S. aureus. This article reviews the epidemiology, risk factors, clinical presentation, and treatment of community-acquired methicillin-resistant S. aureus. 相似文献
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100.
Jind?ich Chrtek Jr Jaroslav Zahradní?ek Karol Krak Judith Fehrer 《Annals of botany》2009,104(1):161-178