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31.
The case for a polyphyletic origin of mitochondria: Morphological and molecular comparisons 总被引:2,自引:0,他引:2
The comparative morphology and pigmentation of protists suggest that those with tubular mitochondrial cristae belong to a different lineage than those with lamellar cristae and that the evolutionary divergence might have been very early. We propose that the difference in cristal morphology is the result of separate origins of the mitochondria from endosymbionts related to the Rhodospirillaceae (purple nonsulfur bacteria) but differing in the morphology of their internal membranes. Comparisons of the cytochromes c of protists and the Rhodospirillaceae and of 16s rRNA T1 oligonucleotide catalogs in the Rhodospirillaceae do not contradict, and in fact provide support for, the idea. More extensive evidence may be lacking simply because cytochromes c have been studied in very few protists with tubular mitochondrial cristae. 相似文献
32.
To determine whether the enhanced insulin-sensitivity of glucose metabolism in muscle after acute exercise also extends to protein metabolism, untrained and exercise-trained rats were subjected to an acute bout of exercise, and the responses of protein synthesis and degradation to insulin were measured in epitrochlearis muscles in vitro. Acute exercise of both untrained and trained rats decreased protein synthesis in muscle in the absence or presence of insulin, but protein degradation was not altered. Exercise training alone had no effect on protein synthesis or degradation in muscle in the absence or presence of insulin. Acute exercise or training alone enhanced the sensitivities of both protein synthesis and degradation to insulin, but the enhanced insulin-sensitivities from training alone were not additive to those after acute exercise. These results indicate that: a decrease in protein synthesis is the primary change in muscle protein turnover after acute exercise and is not altered by prior exercise training, and the enhanced insulin-sensitivities of metabolism of both glucose and protein after either acute exercise or training suggest post-binding receptor events. 相似文献
33.
Summary A vasopressin anti-idiotype antibody was generated by immunization with purified IgG of a primary vasopressin antiserum. The anti-idiotype antibody immunostained neurons in the supraoptic and paraventricular nuclei of the hypothalamus of normal and Brattleboro rats. The distribution of immunostained perikarya in these hypothalamic nuclei together with the staining of fibers in median eminence and neural lobe was similar to that observed in normal rats with anti-vasopressin and suggests strongly that vasopressinergic neurons are being stained. Absorption studies with vasopressin and a vasopressin-binding receptor protein further indicate that a receptor associated with vasopressinergic neurons is recognized by the anti-idiotype antibody.Supported by NIH grants ES03239, NS18626 and NSF grant BNS-8310914. D.T.P. is the receipient of RCDA award NS00869 相似文献
34.
Robert Huber Thomas A. Langworthy Helmut König Michael Thomm Carl R. Woese Uwe B. Sleytr Karl O. Stetter 《Archives of microbiology》1986,144(4):324-333
A novel type of bacterium has been isolated from various geothermally heated locales on the sea floor. The organisms are strictly anaerobic, rod-shaped, fermentative, extremely thermophilic and grow between 55 and 90°C with an optimum of around 80°C. Cells show a unique sheath-like structure and monotrichous flagellation. By 16S rRNA sequencing they clearly belong to the eubacteria, although no close relationship to any known group could be detected. The majority of their lipids appear to be unique in structure among the eubacteria. Isolate MSB8 is described as Thermotoga maritima, representing the new genus Thermotoga.Dedicated to Otto Kandler on the occasion of his 65th birthday
Present addresses: University of South Dakota, Vermillion, USA; University of Illinois, Urbana, USA; Universität für Bodenkultur, Wien, Austria 相似文献
35.
Ten strains representing a novel genus of marine thermophilic archaebacteria growing at between 70 and 103°C with an optimal growth temperature of 100°C and a doubling time of only 37 min were isolated from geothermally heated marine sediments at the beach of Porto di Levante, Vulcano, Italy. The organisms are spherical-shaped, 0.8 to 2.5 m in width and exhibit monopolar polytrichous flagellation. They are strictly anaerobic heterotrophs, growing on starch, maltose, peptone and complex organic substrates. Only CO2 and H2 could be detected as metabolic products, the latter being inhibitory to growth at high concentrations. Hydrogen inhibition can be prevented by the addition of So, whereupon H2S is formed in addition, most likely as the result of a detoxification reaction. The GC-content of the DNA of isolate Vc 1 is 38 mol%. The new genus is named Pyrococcus, the fireball. Type species and strain is Pyrococcus furiosus Vc 1 (DSM 3638). 相似文献
36.
Serge Fermandjian Constantin Sakarellos Franlois Piriou Michel Juy Flavio Toma Hung Lam Thanh Karl Lintner Mahesh C. Khosla Robert R. Smeby F. Merlin Bumpus 《Biopolymers》1983,22(1):227-231
The conformation–biological activity relationships in a series of angiotensin II analogs substituted in position 5 were studied. Results indicated that only analogs with β-branched residue in position 5 possess spectral and biological properties identical to that of parent angiotensin II. 相似文献
37.
Cytokinesis essentially similar to that of vascular plants occurs in Ulothrix, an unbranched filamentous green alga. Plasmodesmata, similar to those of vascular plants, but different from those of many other algae, are also present. Cell plate formation and plasmodesmata also occur in Stigeoclonium, a branched green alga. 相似文献
38.
Saturated Fatty Acid Mutant of Saccharomyces cerevisiae with an Intact Fatty Acid Synthetase 总被引:5,自引:0,他引:5 下载免费PDF全文
To determine directly the effects of streptomycin on translational fidelity in intact cells, we studied the synthesis of beta-galactosidase and of the coat protein of bacteriophage R17 in an Escherichia coli mutant in which the bactericidal effects of streptomycin are delayed. After the addition of streptomycin to exponentially growing mutant cells, protein synthesis continues at an undiminished rate for approximately an hour; however, as measured by enzyme assays, little functional protein is produced. Serological assays designed to detect beta-galactosidase and bacteriophage R17 coat protein show that substantial amounts of the protein synthesized can react with antisera prepared against active beta-galactosidase and phage R17, indicating the aberrance of the protein produced in the presence of the antibiotic. The polypeptides synthesized in the presence of streptomycin are degraded in the cell to a much greater extent than protein synthesized in the absence of the antibiotic. The proteolytic attack on this protein is not affected by inhibitors of serine proteases, suggesting that enzymes other than those involved in "normal turnover" of cellular protein are responsible. In this strain, certain of the multiple effects of streptomycin are separated in time and the production of abnormal protein (enzymatically inactive and susceptible to proteolytic attack) could be studied in the absence of the lethal effect of the drug. 相似文献
39.
Kenzo Sato Karl J. Ullrich 《Biochimica et Biophysica Acta (BBA)/General Subjects》1974,343(3):609-614
Isotonic reabsorption by the rat kidney proximal tubule was drastically inhibited after less than 2 min intraluminal perfusion with fresh sera from rat (both homologous and autologous), cat, rabbit and human, but not with sera from mouse and guinea pig. The inhibitory factor in serum in a heat (56° C for 30 min) and storage (4°C for 2–5 days) labile macromolecule (mol. wt 50 000) and requires Ca2+ for its effect. The cellular electrical potential difference of the proximal tubular cells was irreversively destroyed and intraluminally perfused trypan blue dye incorporated into the tubular cells after the intraluminal perfusion with serum for 2 min. These observations suggest that lysis of the proximal tubular cells is the mechanism for serum-induced inhibition of proximal tubular isotonic reabsorption. 相似文献
40.
Karl -Heinz Wrobel 《Cell and tissue research》1969,101(4):607-620
Zusammenfassung Das Parenchym der Glandula bulbourethralis der Katze besteht aus weitlumigen, gebuchteten intraglandulären Gängen, in welche kurze, englumige, zumeist unverzweigte Tubuli einmünden. Der Drüse fehlt eine äußere Organkapsel, so daß ihre peripheren Tubuli stellenweise direkt zwischen den Fasern des quergestreiften M. bulboglandularis liegen. Die Drüsentubuli und die Buchten der intraglandulären Gänge sind mit einem einschichtigen Zylinderepithel ausgekleidet, auf den Gangfalten ist das Epithel abschnittsweise mehrreihig, Die sezernierende Epitheloberfläche ist durch die Ausbildung von interzellulären Sekretkapillaren vergrößert. Breite Zwischenzellspalten (Durchmesser etwa 1,5), in welche schlanke interdigitierende Cytoplasmafortsätze hineinragen, erstrecken sich von der Basalmembran bis kurz unter das Tubulusbzw. Ganglumen. Die lumenseitigen Zellgrenzen tragen einige stummelförmige Mikrovilli und besitzen zerklüftete Außenkonturen, die durch glykogenreiche Cytoplasmaprojektionen bedingt sind. Alle Epithelzellen sind reich an Mitochondrien. Die supranuklearen Abschnitte der meisten Gang- und Tubuluszellen enthalten Sekretgranula, welche im Elektronenmikroskop unterschiedliche optische Dichten aufweisen können. Die Granula enthalten ein PAS-positives, neuraminsäurehaltiges epitheliales Muzin, das in einzelnen Sekretkörnchen auch eine histochemische Reaktion auf Sulfatgruppen gibt. Alle Epithelzellen reagieren sehr stark auf unspezifische Esterase und stark auf -D-Glucuronidase, -D-Glactosidase sowie die Enzyme des Citronensäurezyklus, der Glykolyse und der Atmungskette (NAD-ICDH, SDH, ALD, LDH, ADH, GDH, NADH-T-Red, Cyt-Ox).
Morphological studies on the bulbourethral gland of the male cat
Summary The bulbourethral glands of sexually mature male cats are studied with the light and electron microscope. The parenchyma consists of spacious, sinus-like intraglandular ducts and short, narrow, mostly unbranched tubular endpieces. The gland has no complete connective tissue capsule, consequently some of the peripheral tubules are situated directly in between the fibers of the surrounding bulboglandularis muscle. The endpieces and the sinus of the intraglandular ducts are lined by a simple columnar epithelium, whereas the folds of the ducts are generally covered by a low pseudostratified epithelium. The secretory surface of the cells is increased by intercellular canaliculi which communicate with the gland lumen. These canaliculi are identified on the light microscopic level by their strong 5-nucleotidase activity. Furthermore widened intercellular spaces (approximately 1,5 in diameter) filled with slender, interdigitating cytoplasmic processes extend from the basal lamina to the apical junctional complexes. The luminal cell pole exhibits some short microvilli and forms irregularly shaped, glycogen containing protrusions. Within the cytoplasm of the gland cells numerous spherical mitochondria, some dense bodies, a typical Golgi apparatus, free ribosomes and a poorly developed endoplasmic reticulum are to be observed. Secretory granules which can be grouped into three types on the basis of their electron density occur in the supranuclear regions of most of the cells. According to histochemical tests all granules contain a periodate reactive sialomucin and some of them also sulfate groups. The glandular parenchyma is site of an exceptionally strong unspecific esterase activity and is rich in -D-glucuronidase, -D-glactosidase, aldolase, -glycerophosphate dehydrogenase, lactate dehydrogenase, alcohol dehydrogenase, NAD-dependent isocitrate dehydrogenase, succinate dehydrogenase and cytochrome oxydase.
Mit dankenswerter Unterstützung durch die Deutsche Forschungsgemeinschaft. 相似文献