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111.
Koski MK Hieta R Böllner C Kivirikko KI Myllyharju J Wierenga RK 《The Journal of biological chemistry》2007,282(51):37112-37123
Prolyl 4-hydroxylases (P4Hs) are 2-oxoglutarate dioxygenases that catalyze the hydroxylation of peptidyl prolines. They play an important role in collagen synthesis, oxygen homeostasis, and plant cell wall formation. We describe four structures of a P4H from the green alga Chlamydomonas reinhardtii, two of the apoenzyme at 1.93 and 2.90 A resolution, one complexed with the competitive inhibitor Zn2+, and one with Zn2+ and pyridine 2,4-dicarboxylate (which is an analogue of 2-oxoglutarate) at 1.85 A resolution. The structures reveal the double-stranded beta-helix core fold (jellyroll motif), typical for 2-oxoglutarate dioxygenases. The catalytic site is at the center of an extended shallow groove lined by two flexible loops. Mutagenesis studies together with the crystallographic data indicate that this groove participates in the binding of the proline-rich peptide-substrates. It is discussed that the algal P4H and the catalytic domain of collagen P4Hs have notable structural similarities, suggesting that these enzymes form a separate structural subgroup of P4Hs different from the hypoxia-inducible factor P4Hs. Key structural differences between these two subgroups are described. These studies provide first insight into the structure-function relationships of the collagen P4Hs, which unlike the hypoxia-inducible factor P4Hs use proline-rich peptides as their substrates. 相似文献
112.
Bone is a composite composed mainly of organics, minerals, and water. Many researchers have studied effects such as crack velocity, density, orientation, storage media, porosity, and age on the fracture toughness (K(C), also called critical stress intensity factor) of compact bone. Most of these studies were conducted at room temperature. Considering that the body temperature of animals is greater than room temperature, and that bone has a large volumetric percentage of organics and water (generally, 55-65%), it is hypothesized that temperature has a significant effect on the fracture toughness of compact bone. Single-edge V-notched (SEVN) specimens were prepared to measure the fracture toughness of bovine femur and manatee rib in water at 0, 10, 23, 37, and 50 degrees C in four-point flexure. The fracture toughness values of bovine femur and manatee rib were found to decrease from 7.0 to 4.3MPam(1/2) and from 5.5 to 4.0MPam(1/2), respectively, as temperature increased over a temperature range of 50 degrees C. The results support the hypothesis that temperature has a significant effect on the fracture toughness of compact bone. Therefore, we suggest that study on fracture toughness of bone should be done at physiologically relevant temperatures. 相似文献
113.
Assarsson E Chambers BJ Högstrand K Berntman E Lundmark C Fedorova L Imreh S Grandien A Cardell S Rozell B Ljunggren HG 《Journal of immunology (Baltimore, Md. : 1950)》2007,178(8):5018-5027
Transgenic mice were generated expressing NK1.1, an NK cell-associated receptor, under control of the human CD2 promoter. Unexpectedly, one of the founder lines, Tg66, showed a marked defect in thymic development characterized by disorganized architecture and small size. Mapping of the transgene insertion by fluorescence in situ hybridization revealed integration in chromosome 2, band G. Already from postnatal day 3, the thymic architecture was disturbed with a preferential loss of cortical thymic epithelial cells, a feature that became more pronounced over time. Compared with wild-type mice, total thymic cell numbers decreased dramatically between 10 and 20 days of age. Thymocytes isolated from adult Tg66 mice were predominantly immature double-negative cells, indicating a block in thymic development at an early stage of differentiation. Consequently, Tg66 mice had reduced numbers of peripheral CD4(+) and CD8(+) T cells. Bone marrow from Tg66 mice readily reconstituted thymi of irradiated wild-type as well as RAG-deficient mice. This indicates that the primary defect in Tg66 mice resided in nonhemopoietic stromal cells of the thymus. The phenotype is observed in mice heterozygous for the insertion and does not resemble any known mutations affecting thymic development. Preliminary studies in mice homozygous for transgene insertion reveal a more accelerated and pronounced phenotype suggesting a semidominant effect. The Tg66 mice may serve as a useful model to identify genes regulating thymic epithelial cell differentiation, thymic development, and function. 相似文献
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115.
Ana Carolina Luchiari Cristiane Regina do Amaral Duarte Fúlvio Aurélio de Morais Freire Kari Nissinen 《Journal of Ethology》2007,25(2):169-175
We studied the colour preference of isolated Nile tilapia (Oreochromis niloticus) and whether previous residence or body size can affect environmental colour choice. In the first phase, a cylindrical tank
was divided into five differently coloured compartments (yellow, blue, green, white and red), a single fish was introduced
into the tank and the frequency at which this fish visited each compartment was recorded over a 2-day study period. An increasingly
larger fish (approx +2 cm in length each time) was then added into the tank on each of days 3, 5 and 7 (=four fish in the
tank by day 7), and the frequency at which each fish visited the different compartments of the tank was observed twice a day
to obtain visit frequency data on the differently sized fishes. This experiment was replicated six times. In the first phase,
the solitary fish established residence inside the yellow compartment on the first and second days. Following the
introduction of a larger fish, the smaller fish was displaced from the occupied compartment. Nile tilapia possibly shows this
preference for yellow as a function of its visual spectral sensitivity and/or the spectral characteristics of its natural
environment. Moreover, body size is an important factor in determining hierarchical dominance and territorial defence, and
dominant fish chose the preferred environmental colour compartment as their territory. 相似文献
116.
Lynch AI Arnett DK Pankow JS Miller MB North KE Eckfeldt JH Hunt SC Rao DC Djoussé L 《Human genetics》2007,122(1):33-40
Evidence shows that an elevated pulse pressure (PP) may lead to an increased risk of cardiovascular morbidity and mortality.
There is also evidence that PP is a sexually dimorphic trait, and that genetic factors influence inter-individual variation
in PP. The aim of this project was to assess the genotype-by-sex interaction on PP in a sample of mostly hypertensive African
American and White participants using candidate genes involved in the renin–angiotensin–aldosterone system. Subjects were
participants in the HyperGEN Study, including men (43%) and women (57%) over the age of 55 years (mean age = 65). Candidate
gene polymorphisms used were ACE insertion/deletion (1,789 subjects genotyped) and AGT-M235T (1,800 subjects genotyped). We employed linear regression methods to assess the genotype-by-sex interaction. For ACE, genotype-by-sex interaction on PP was detected (P = 0.04): the “D/D” genotype predicted a 2.2 mmHg higher pulse pressure among women, but a 1.2 mmHg lower PP among men, compared to those with an “I” allele, after adjusting for age, weight, height, ethnicity, and antihypertension
medication use. A similar interaction was found for systolic blood pressure. The genotype-by-sex interaction was consistent
across ethnicity. The interaction was evident among those on antihypertensive medications (P = 0.05), but not among those not taking such medications (P = 0.55). In our analysis of AGT, no evidence of a genotype-by-sex interaction affecting PP, SBP, or DBP was detected. This evidence for a genotype-by-sex
interaction helps our understanding of the complex genetic underpinnings of blood pressure phenotypes. 相似文献
117.
Alanne M Kristiansson K Auro K Silander K Kuulasmaa K Peltonen L Salomaa V Perola M 《Human genetics》2007,122(3-4):355-365
Selenoprotein S (SEPS1) is a novel candidate gene involved in the regulation of inflammatory response and protection from
oxidative damage. This study explored the genetic variation in the SEPS1 locus for an association with CVD as well as with
quantitative phenotypes related to obesity and inflammation. We used the case-cohort design and time-to-event analysis in
two separate prospectively followed population-based cohorts FINRISK 92 and 97 (n = 999 and 1,223 individuals, respectively) to study the associations of five single nucleotide polymorphisms with the risk
for coronary heart disease (CHD) and ischemic stroke events. We found a significant association with increased CHD risk in
females carrying the minor allele of rs8025174 in the combined analysis of both cohorts [hazard ratio (HR) 2.95 (95% confidence interval: 1.37–6.39)]. Another variant,
rs7178239, increased the risk for ischemic stroke significantly in females [HR: 3.35 (1.66–6.76)] and in joint analysis of both sexes
and both cohorts [HR: 1.75 (1.17–2.64)]. These results indicate that variation in the SEPS1 locus may have an effect on CVD
morbidity, especially in females. This observation should stimulate further investigations of the role of this gene and protein
in the pathogenesis of CVD.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
118.
The Arabidopsis transcription factor MYB77 modulates auxin signal transduction 总被引:6,自引:0,他引:6
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Shin R Burch AY Huppert KA Tiwari SB Murphy AS Guilfoyle TJ Schachtman DP 《The Plant cell》2007,19(8):2440-2453
119.
120.
Trager EH Khanna R Marrs A Siden L Branham KE Swaroop A Richards JE 《Bioinformatics (Oxford, England)》2007,23(14):1854-1856
The Madeline 2.0 Pedigree Drawing Engine (PDE) is a pedigree drawing program for use in linkage and family-based association studies. The program is designed to handle large and complex pedigrees with an emphasis on readability and aesthetics. For complex pedigrees, we use a hybrid algorithm in which consanguinous loops are drawn as cyclic graphs whenever possible, but we resort to acyclic graphs when matings can no longer be connected without line crossings. A similar hybrid approach is used to avoid line crossings for matings between distant descendants of different founding groups. Written in object-oriented C++ and released under the GNU General Public License (GPL), Madeline 2.0 PDE reads input files specified on the command line and generates pedigree drawings without user interaction. Pedigree output in scalable vector graphics (SVG) format can be viewed in browsers with native SVG rendering support or in vector graphics editors. We provide an easy-to-use public web service, which is experimental and still under development. Availability: http://kellogg.umich.edu/madeline. 相似文献