Dynactin-dependent cortical dynein and spherical spindle shape correlate temporally with meiotic spindle rotation in Caenorhabditis elegans

Oocyte meiotic spindles orient with one pole juxtaposed to the cortex to facilitate extrusion of chromosomes into polar bodies. In Caenorhabditis elegans, these acentriolar spindles initially orient parallel to the cortex and then rotate to the perpendicular orientation. To understand the mechanism of spindle rotation, we characterized events that correlated temporally with rotation, including shortening of the spindle in the pole-to pole axis, which resulted in a nearly spherical spindle at rotation. By analyzing large spindles of polyploid C. elegans and a related nematode species, we found that spindle rotation initiated at a defined spherical shape rather than at a defined spindle length. In addition, dynein accumulated on the cortex just before rotation, and microtubules grew from the spindle with plus ends outward during rotation. Dynactin depletion prevented accumulation of dynein on the cortex and prevented spindle rotation independently of effects on spindle shape. These results support a cortical pulling model in which spindle shape might facilitate rotation because a sphere can rotate without deforming the adjacent elastic cytoplasm. We also present evidence that activation of spindle rotation is promoted by dephosphorylation of the basic domain of p150 dynactin.     

Was monogamy a key step on the hominin road? reevaluating the monogamy hypothesis in the evolution of cooperative breeding

Because human mothers routinely rely on others to help raise their young, humans have been characterized as cooperative breeders.^1–9 Several large‐scale phylogenetic analyses have presented compelling evidence that monogamy preceded the evolution of cooperative breeding in a wide variety of nonhuman animals.^10–14 These studies have suggested that monogamy provides a general rule (the monogamy hypothesis) for explaining evolutionary transitions to cooperative breeding.¹⁵ Given the prevalence of cooperative breeding in contemporary human societies, we evaluate whether this suggests a monogamous hominin past.     

Hydrological responses of a valley‐bottom wetland to land‐use/land‐cover change in a South African catchment: making a case for wetland restoration

Valley‐bottom wetlands are valuable assets as they provide many ecosystem services to mankind. Despite their value, valley‐bottom wetlands are often exploited and land‐use/land‐cover (LULC) change results in trade‐offs in ecosystem services. We coupled physically based hydrological modeling and spatial analysis to examine the effects of LULC change on water‐related ecosystem services in the Kromme catchment: an important water‐providing catchment for the city of Port Elizabeth. LULC scenarios were constructed to match 5 different decades in the last 50 years to explore the potential effects of restoring the catchment to different historic benchmarks. In the Kromme catchment, valley‐bottom wetlands have declined by 84%, driven by key LULC changes: an increase in irrigated land (307 ha) and invasion by alien trees (336 ha). If the wetlands were restored to the relatively pristine extent and condition of the 1950s, riverflow could increase by approximately 1.13 million m³/a, about 6% of the current supply to Port Elizabeth. Wetland restoration would also significantly improve the catchment's ability to absorb extreme rainfall events, decreasing flood damage. We conclude that in the face of the water scarcity in this region, all ecosystem services, particularly those related to water flow regulation, should be taken into account by decision makers in charge of land zonation. Zonation decisions should not continue to be made on the basis of provisioning ecosystem services alone (i.e. food provision or dam yield). We recommend prioritization of the preservation and restoration of valley‐bottom wetlands providing water‐related ecosystem services to settlements downstream.     

Implementing Evidence-Based Programs: Lessons Learned From the Field

Evidence-based programs (EBPs) are used in many health promotion efforts to ensure that the intended positive behavioral and health outcomes will be achieved. However, because EBPs are developed and tested in research settings, the contextual elements of real world implementation play an important role in their successful delivery in communities. As an intermediary charged with supporting the translation of research to practice for a statewide teen pregnancy prevention initiative, the ACT for Youth Center of Excellence conducted a mid-course evaluation to identify factors that contribute to the successful implementation of EBPs. The findings indicate that successful implementation involves systematic planning that addresses three critical contextual factors: community readiness, organizational capacity, and setting characteristics. By attending both to factors identified from local practice, and to theory and evidence identified through implementation science, practitioners can strengthen programming and enhance the quality of EBP delivery.     

Functional relevance of “seed” and “non-seed” sequences in microRNA-mediated promotion of C. elegans developmental progression

The founding heterochronic microRNAs, lin-4 and let-7, together with their validated targets and well-characterized phenotypes in C. elegans, offer an opportunity to test functionality of microRNAs in a developmental context. In this study, we defined sequence requirements at the microRNA level for these two microRNAs, evaluating lin-4 and let-7 mutant microRNAs for their ability to support temporal development under conditions where the wild-type lin-4 and let-7 gene products are absent. For lin-4, we found a strong requirement for seed sequences, with function drastically affected by several central mutations in the seed sequence, while rescue was retained by a set of mutations peripheral to the seed. let-7 rescuing activity was retained to a surprising degree by a variety of central seed mutations, while several non-seed mutant effects support potential noncanonical contributions to let-7 function. Taken together, this work illustrates both the functional partnership between seed and non-seed sequences in mediating C. elegans temporal development and a diversity among microRNA effectors in the contributions of seed and non-seed regions to activity.