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991.
We assessed the vertical growth and mycorrhizal infection of woody plant roots on a closed landfill, using tree and shrub clusters that had been previously installed in patches of increasing size to establish protocols for woodland restoration. The density of the fine roots of shrubs, which had poor-to-moderate mycorrhizal infection, decreased strongly with increasing depth. Oak ( Quercus ) seedlings planted within and outside patches were assessed for ectomycorrhizal infection. Oak root systems were mycorrhizal, but root-tip proliferation was improved and ectomycorrhizal composition was influenced by woody debris in the mineral soil. Most surviving oaks were found within patches, but all seedlings showed poor growth: most taproots were deflected horizontally above the boundary between surface soil and subsoil layers (−15 cm). Abrupt decreases in pH between surface and subsurface horizons (6.9 versus 5.3), together with poor drainage and aeration of the latter soil, were probably responsible for poor root growth. Root growth of greenhouse-grown pine and maple seedlings was similarly restricted in pots packed with topsoil over subsoil material. Our results suggest that many current specifications for the cover of closed landfills will not permit restoration of native woody plant communities because of physical limitations to root growth and infectivity. The structure of the engineered soil must address basic plant growth requirements as well as traditional concerns of drainage and barrier protection. 相似文献
992.
Do Bird Perching Structures Elevate Seed Rain and Seedling Establishment in Abandoned Tropical Pasture? 总被引:14,自引:0,他引:14
Karen D. Holl 《Restoration Ecology》1998,6(3):253-261
Lack of seed dispersal has been shown to be a major factor limiting tropical forest recovery in abandoned pasture land. The goal of this work was to determine whether bird perching structures serve to enhance seed dispersal and seedling establishment in an abandoned pasture in Costa Rica. Two types of perching structures (crossbar and branch) were tested. Bird visitation rates were significantly higher on branch than on crossbar perches. The number of animal-dispersed seeds was significantly higher below branch perches than below crossbar perches or in open pasture. Despite differences in seed rain, percent cover of animal-dispersed plants and the number of seedlings of animal-dispersed plant species were similar below both perch types and in open pasture. Baiting perches with bananas did not increase either bird visitation rates or seed rain. These results suggest that, although bird perching structures increase seed dispersal, they do not overcome other barriers to tropical forest recovery such as seed predation and low seed germination. 相似文献
993.
Walter E. Horton Jr. Imelda Udo Patricia Precht Richard Blakir Karen Hasty 《In vitro cellular & developmental biology. Animal》1998,34(5):378-384
Summary The objective of this study was to determine if an immortalized mammalian chondrocyte cell line had a profile of matrix metalloproteinase
(MMP) expression that was consistent with what has been reported for primary chondrocytes in vitro and in vivo. A combination of zymography, Western, and Northern analysis was used to examine the expression of MMPs that are relevant
to cartilage degradation. Both interleukin-1β and tumor necrosis factor α induced a 4- to 9-fold increase in the level of
MMP-9 expression in conditioned media, and a 17- to 24-fold increase in MMP-3 mRNA. Other compounds such as basic fibroblast
growth factor and staurosporine each increased MMP-9 expression individually and potentiated the effects of the two cytokines.
Transforming growth factor β had no positive or inhibitory effects. N-methyl arginine blocked the increase in nitric oxide observed following treatment with the cytokines but did not prevent
the increased expression of MMPs. The pattern of metalloproteinase expression observed in IRC cells and the response to cytokines
is very similar to what has been reported during the pathogenesis of osteoarthritis. The IRC cells should be useful as a model
system to study basic mechanisms controlling chondrocyte MMP expression and to identify pharmacological modulators of this
process. 相似文献
994.
995.
996.
Aerobic exercise maintains regional bone mineral density during weight loss in postmenopausal women 总被引:2,自引:0,他引:2
Ryan Alice S.; Nicklas Barbara J.; Dennis Karen E. 《Journal of applied physiology》1998,84(4):1305-1310
This studyexamines the effects of weight loss by caloric restriction (WL) andaerobic exercise plus weight loss (AEx+WL) on total and regional bonemineral density (BMD) in older women. Healthy,postmenopausal women [age 63 ± 1 (SE) yr] not onhormone-replacement therapy underwent 6 mo of WL(n = 15) consisting of dietarycounseling one time per week with a caloric deficit (250-350kcal/day) or AEx+WL (n = 15)consisting of treadmill exercise three times per week in addition tothe weight loss. Maximal aerobic capacity increased only in the AEx+WLgroup (P < 0.001). Body weight,percent fat, and fat mass decreased similarly in both groups(P < 0.005), with no changesin fat-free mass. Total body BMD (by dual-energy X-rayabsorptiometry) decreased in both groups(P < 0.05). Femoral neck, Ward'striangle, and greater trochanter BMD decreased in the WL group(P 0.05) but were not significantlydifferent after AEx+WL.L2-L4BMD did not significantly change in either group. Thus WL andAEx+WL both result in losses of totalbody BMD; however, AEx+WL appears to prevent the loss in regional BMDseen with WL alone in healthy, older women. This suggests that theaddition of exercise to weight-loss programs may reduce the risk forbone loss. 相似文献
997.
The ability to predict the effects of climate change on trace gas fluxes requires a knowledge of microbial temperature responses. However, the response of a microbial community to temperature in a given substrate may be complicated by its thermal history. To examine the effect of sequentially changing temperature on methane and carbon dioxide production in different peat types, we incubated anaerobic peat samples from 3 types of northern peatlands, a bog, a sedge fen and a cedar swamp, in both rising and falling temperature regimes. Graphic and statistical comparisons of the different temperature regimes suggest hysteresis in microbial response to temperature, although the absolute rates at any given temperature often did not differ. Where regressions for temperature response (Arrhenius plots) were significant, they generally differed between temperature regimes. The greatest differences among treatments occurred during the first half of the 40-d incubation. Increases in carbon dioxide production were similar across all peat types, but methanogenesis varied widely: methane production was uniformly low in the bog peat but increased sharply with temperature in the other two peat types. The complicating effect of history or chronology on substrate responses to environmental stimuli may restrain our ability to model the responses of complex systems to changing conditions. 相似文献
998.
999.
Uribe Xavier Torres Miguel Angel Capellades Montserrat Puigdomènech Pere Rigau Joan 《Plant molecular biology》1998,37(6):1069-1078
In the past few years many - and -tubulin genes of different organisms have been cloned and studied, and in most systems studied they constitute multigene families. In plants, most studies have been done in Arabidopsis thaliana and Zea mays. In this paper, the study of mRNA accumulation by in situ hybridization and the activity of three maize -tubulin gene promoters (tua1, tua2 and tua3) in transgenic tobacco plants are described. In maize, the expression of these three tubulin isotypes differ in the root and shoot apex and is associated with different groups of cells throughout the distinct stages of cell differentiation. In transgenic tobacco plants the promoters of the genes, fused to the uidA reporter gene (GUS), direct expression to the same tissues observed by in situ hybridization experiments. The tua1 promoter is mainly active in cortex-producing meristematic cells and in pollen, whereas tua3 is active in cells which are differentiating to form vascular bundles in the root and shoot apices. The accumulation of tua2 mRNA is detected by RNA blot in a similar form as tua1, but at a very much low level. In situ hybridization indicates that the tua2 mRNA specifically accumulates in the maize root epidermis. No GUS staining was detected in transgenic tobacco plants with the tua2 promoter. The difference in expression of the specific genes may be linked to processes where microtubules have different functions, suggesting that in plants, as in animals, there are differences in the function of the tubulin isotypes. 相似文献
1000.