Changes in the biological activity of agropyrene on combined application with humus substances in the cultivation ofScenedesmus obliquus (TURP.) krüger

The work is concerned with the question of the effect of humic acids on the biological action of agropyrene, the effective substance from the rootstock of Agropyron repens (L.) P. BEAUV. The test organism was the alga Scenedesmus obliquus (TURP.) KRÜGER, cultivated in mineral solution. The basic biological test was the determination of the number and size of the cells of this alga. It was found that the way in which agropyrene acts on combined application with humus acids depends on which humus fraction is used. It can display a synergic action if humic acid is used, since this substance probably facilitates the penetration of agropyrene into the cells. On the other hand, fulvic acid counteracts the activity of agropyrene and this most probably by mutual binding and blocking of active groups and links.     

Die durch Blattdüngung mit Humusstoffen hervorgerufenen anatomischen und physiologischen Veränderungen der Zuckerrübe

V p?edlo?ené práci je sledován ú?inek humusových látek aplikovaných na listy cukrovky post?ikem. Sou?asně je ově?ována vhodnost kombinace humusových látek s minerálními ?ivinami. Ukazuje se, ?e post?ik humusovými látkami zvlá?tě v kombinaci s minerálními ?ivinami p?íznivě ovlivňuje r?st cukrovky, zvy?uje váhu list? i ko?ene a celkové mno?ství cukru v ko?eni. Ú?inok post?iku humusovými látkami je vět?í u rostlin pěstovaných ve vodní kultu?e a st?íkaných ?ivným roztokem s kompletněj?ím zastoupením minerálních prvk?. Humusové látky p?i aplikaci na list vyvolávají podobné změny v anatomické stavbě pletiv a orgán?, jako p?i jejich aplikaci do ?ivného roztoku ke ko?en?m. Humusové látky zvlá?tě v kombinaci s minerálním roztokem zvy?ují v listech cukrovky mno?ství chlorofylu a zvy?ují intezitu fotosyntézy. Post?ik humusovými látkami zvy?uje sou?asně transpiraci cukrovky.     

The influence of EDTA on the mutagenic activity of ethyl methanesulphonate (EMS) inArabidopsis thaliana

Nebyl prokázán sensibilizující ú?inek EDTA na muta?ní aktivitu EMS uArabidopsis thaliana.     

Biomass partitioning in twoCalamagrostis villosa stands on deforested sites

Calamagrostis villosa stands occurring in areas deforested by air-pollution impact in the Moravian-Silesian Beskydy Mountains were characterized by a high dry mass of total underground biomass (3 300 g. m^?2—the slope site, 2 850 g. m^?2—the flat site). The percentage of living roots and rhizomes in total underground biomass was very high (about 70%). The total aboveground biomass was respectively, 321 g.m^?2 (the slope site) and 726 g. m^?2 (the flat site). In unstabilized habitats on steep slope, the higher plant biomass produced was allocated to a more developed root system.     

Catalysis by Laccase (From Coriolus uersicolor) in Microheterogeneous Media of the Water/Organic Solvent/Surfactant Type

Catalysis by laccase from Coriolus uersicolor solubilized in the ternary systems of surfactant/water/organic solvent type, namely, Aerosol OT/water/octane, Brij 56/water/cyclohexane and egg lecithin/water/octane + pentanol + methanol mixture, has been studied. The laccase activity is found to depend, in principle, not only on the water/surfactant molar ratio, but on the surfactant concentration (with its hydration degree being constant) as well. The following inferences should be emphasized. Firstly, in all the systems under study, the catalytic activity (k_cat) of laccase entrapped into surfactant reversed micelles increases more than 50 times (when the surfactant concentration is extrapolated to zero) compared with the k_cat value in aqueous solution. Secondly, the catalytic activity (k_cat) of laccase entrapped in hydrated Aerosol OT aggregates, having lamellar, reversed cylindrical (hexagonal) and reversed micellar structure, depends greatly on the aggregate type. In other words, the phase transitions, i.e. an alteration in the packing of hydrated Aerosol OT molecules, evokes a sharp reversible change in the enzymatic activity. Thirdly, in the same phase, the catalytic activity of the solubilized enzyme depends on the linear dimensions of water cavities inside the surfactant aggregates (i.e. on the water content in the system under study). All these effects, regulating enzymatic activity, are probably caused by an alteration of the conformational mobility of laccase molecules incorporated into the inner polar cavities inside the surfactant aggregates.