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11.
Dominique Hervé Anne Philippi Reda Belbouab Michel Zerah Stéphane Chabrier Sophie Collardeau-Frachon Francoise Bergametti Aurore Essongue Eliane Berrou Valérie Krivosic Christian Sainte-Rose Emmanuel Houdart Frédéric Adam Kareen Billiemaz Marilyne Lebret Sabine Roman Sandrine Passemard Gwenola Boulday Audrey Delaforge Stéphanie Guey Xavier Dray Hugues Chabriat Peter Brouckaert Maryjke Bryckaert Elisabeth Tournier-Lasserve 《American journal of human genetics》2014,94(4):642
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Thierry Darribère Jean-François Riou Kareen Guida Anne-Marie Duprat Jean-Claude Boucaut Jean-Claude Beetschen 《Cell and tissue research》1991,263(3):507-514
Summary In the early development of the urodele amphibian Pleurodeles waltl, a fibronectin-containing extracellular matrix underlies the inner face of the blastocoel roof. When gastrulation occurs, the fibronectin fibrils provide a suitable substrate for mesodermal-cell migration. Delay in morphogenetic movements of gastrulation has been described in embryos from mutant females (ac/ac) of Pleurodeles waltl. Studies of abnormal mutant gastrulae with fluorescent lectins and immunostaining for fibronectin reveal that they lack a normal matrix. The fibronectin-containing extracellular material always gives rise to a granular pattern without fibronectin-fibril formation. Fibronectin and 51 syntheses occur normally in maternal-effect embryos. In vitro, mesodermal cells from early mutant gastrulae adhere and migrate on fibronectin-conditioned substrata. 相似文献
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Cardiac length dependence of force and force redevelopment kinetics with altered cross-bridge cycling 下载免费PDF全文
We examined the influence of cross-bridge cycling kinetics on the length dependence of steady-state force and the rate of force redevelopment (k(tr)) during Ca(2+)-activation at sarcomere lengths (SL) of 2.0 and 2.3 microm in skinned rat cardiac trabeculae. Cross-bridge kinetics were altered by either replacing ATP with 2-deoxy-ATP (dATP) or by reducing [ATP]. At each SL dATP increased maximal force (F(max)) and Ca(2+)-sensitivity of force (pCa(50)) and reduced the cooperativity (n(H)) of force-pCa relations, whereas reducing [ATP] to 0.5 mM (low ATP) increased pCa(50) and n(H) without changing F(max). The difference in pCa(50) between SL 2.0 and 2.3 microm (Delta pCa(50)) was comparable between ATP and dATP, but reduced with low ATP. Maximal k(tr) was elevated by dATP and reduced by low ATP. Ca(2+)-sensitivity of k(tr) increased with both dATP and low ATP and was unaffected by altered SL under all conditions. Significantly, at equivalent levels of submaximal force k(tr) was faster at short SL or increased lattice spacing. These data demonstrate that the SL dependence of force depends on cross-bridge kinetics and that the increase of force upon SL extension occurs without increasing the rate of transitions between nonforce and force-generating cross-bridge states, suggesting SL or lattice spacing may modulate preforce cross-bridge transitions. 相似文献
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Paola Piazza Magdalena B?a?ewicz-Paszkowycz Claudio Ghiglione Maria?Chiara Alvaro Kareen Schnabel Stefano Schiaparelli 《ZooKeys》2014,(451):49-60
Here we present distributional records for Tanaidacea specimens collected during several Antarctic expeditions to the Ross Sea: the Italian PNRA expeditions (“V”, 1989/1990; “XI”, 1995/1996; “XIV”, 1998/1999; “XIX”, 2003/2004; “XXV”, 2009/2010) and the New Zealand historical (New Zealand Oceanographic Institute, NZOI, 1958-1961) and recent (“TAN0402 BIOROSS” voyage, 2004 and “TAN0802 IPY-CAML Oceans Survey 20/20” voyage, 2008) expeditions. Tanaidaceans were obtained from bottom samples collected at depths ranging from 16 to 3543 m by using a variety of sampling gears. On the whole, this contribution reports distributional data for a total of 2953 individuals belonging to 33 genera and 50 species. All vouchers are permanently stored in the Italian National Antarctic Museum collection (MNA), Section of Genoa (Italy) and at the National Institute of Water and Atmospheric Research (NIWA Invertebrate Collection), Wellington (New Zealand). 相似文献
15.
Deborah V. Hoyle Marianne Keith Helen Williamson Kareen Macleod Heather Mathie Ian Handel Carol Currie Anne Holmes Lesley Allison Rebecca McLean Rebecca Callaby Thibaud Porphyre Sue C. Tongue Madeleine K. Henry Judith Evans George J. Gunn David L. Gally Nuno Silva Margo E. Chase-Topping 《Applied and environmental microbiology》2021,87(10)
16.
Liam Nolan Ian D. Hogg Darin L. Sutherland Mark I. Stevens Kareen E. Schnabel 《New Zealand journal of zoology.》2013,40(4):371-380
Abstract We collected larval damselflies from 17 sites in the North, South and Chatham Islands, and tested the hypotheses that: (1) genetic markers (e.g., allozymes, mtDNA) would successfully discriminate taxa; and (2) the dispersal capabilities of adult damselflies would limit differentiation among locations. Four species from three genera were identified based on available taxonomic keys. Using 11 allozyme loci and the mitochondrial cytochrome c‐oxidase subunit I (COI) gene, we confirmed that all taxa were clearly discernible. We found evidence for low to moderate differentiation among locations based on allozyme (meani F ST = 0.09) and sequence (COI) divergence (<0.034). No obvious patterns with respect to geographic location were detected, although slight differences were found between New Zealand's main islands (North Island, South Island) and the Chatham Islands for A. colensonis (sequence divergence 0.030–0.034). We also found limited intraspecific genetic variability based on allozyme data (Hexp < 0.06 in all cases). We conclude that levels of gene flow/dispersal on the main islands may have been sufficient to maintain the observed homogeneous population structure, and that genetic techniques, particularly the COI gene locus, will be a useful aid in future identifications. 相似文献
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Bacitracin interacts with the plasma membranes of gram-positive and gram-negative bacteria to produce morphological changes which appear as rods, 25–35 nm in diameter, and of variable length, in freeze fractured preparations. 相似文献
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Sergio Vargas Michelle Kelly Kareen Schnabel Sadie Mills David Bowden Gert W?rheide 《PloS one》2015,10(6)
Background
The approximately 350 demosponge species that have been described from Antarctica represent a faunistic component distinct from that of neighboring regions. Sponges provide structure to the Antarctic benthos and refuge to other invertebrates, and can be dominant in some communities. Despite the importance of sponges in the Antarctic subtidal environment, sponge DNA barcodes are scarce but can provide insight into the evolutionary relationships of this unique biogeographic province.Methodology/Principal Findings
We sequenced the standard barcoding COI region for a comprehensive selection of sponges collected during expeditions to the Ross Sea region in 2004 and 2008, and produced DNA-barcodes for 53 demosponge species covering about 60% of the species collected. The Antarctic sponge communities are phylogenetically diverse, matching the diversity of well-sampled sponge communities in the Lusitanic and Mediterranean marine provinces in the Temperate Northern Atlantic for which molecular data are readily available. Additionally, DNA-barcoding revealed levels of in situ molecular evolution comparable to those present among Caribbean sponges. DNA-barcoding using the Segregating Sites Algorithm correctly assigned approximately 54% of the barcoded species to the morphologically determined species.Conclusion/Significance
A barcode library for Antarctic sponges was assembled and used to advance the systematic and evolutionary research of Antarctic sponges. We provide insights on the evolutionary forces shaping Antarctica''s diverse sponge communities, and a barcode library against which future sequence data from other regions or depth strata of Antarctica can be compared. The opportunity for rapid taxonomic identification of sponge collections for ecological research is now at the horizon. 相似文献19.
Kareen J. I. Thorne 《The Biochemical journal》1973,135(3):567-568
Growing cells of Streptococcus mutans Ingbritt incorporate radioactive mevalonate into unsaponifiable lipid. Of the radioactive lipid 40% was shown by chromatography and mass spectrometry to be C(50) and C(55) prenol. 相似文献
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