全文获取类型
收费全文 | 3015篇 |
免费 | 173篇 |
国内免费 | 1篇 |
出版年
2023年 | 5篇 |
2022年 | 10篇 |
2021年 | 38篇 |
2020年 | 19篇 |
2019年 | 20篇 |
2018年 | 38篇 |
2017年 | 32篇 |
2016年 | 54篇 |
2015年 | 123篇 |
2014年 | 138篇 |
2013年 | 219篇 |
2012年 | 186篇 |
2011年 | 196篇 |
2010年 | 122篇 |
2009年 | 141篇 |
2008年 | 188篇 |
2007年 | 177篇 |
2006年 | 208篇 |
2005年 | 203篇 |
2004年 | 225篇 |
2003年 | 187篇 |
2002年 | 170篇 |
2001年 | 37篇 |
2000年 | 29篇 |
1999年 | 44篇 |
1998年 | 40篇 |
1997年 | 32篇 |
1996年 | 21篇 |
1995年 | 25篇 |
1994年 | 23篇 |
1993年 | 20篇 |
1992年 | 19篇 |
1991年 | 18篇 |
1990年 | 20篇 |
1989年 | 11篇 |
1988年 | 18篇 |
1987年 | 10篇 |
1986年 | 9篇 |
1985年 | 12篇 |
1984年 | 12篇 |
1983年 | 9篇 |
1982年 | 12篇 |
1981年 | 15篇 |
1979年 | 9篇 |
1978年 | 9篇 |
1976年 | 8篇 |
1973年 | 4篇 |
1971年 | 4篇 |
1969年 | 4篇 |
1968年 | 3篇 |
排序方式: 共有3189条查询结果,搜索用时 15 毫秒
41.
42.
43.
An assay for ferredoxin-glutamate synthase is introduced thatuses an anion exchange resin to isolate the glutamate formedand subsequent determination with the ninhydrin procedure. Theenzyme was purified 200-fold from corn leaves by ammonium sulfatefractionation and chromatography on DEAE-cellulose, DEAE-Sephaceland ferredoxin- Sepharose. The purified enzyme had a specificactivity of 14 µmoles glutamate formed min1mg1protein. The enzyme has a molecular weight of 160,000. The pHoptimum for catalytic activity is 6.9. The isoelectric pointis at pH 4.2. The apparent Km values of the enzyme for L-glutamine,2-oxoglutarate and ferredoxin are 1,100, 240 and 1.7 µM.The enzyme has a high specificity toward these substrates witha stoichiometry between glutamate formation and glutamine consumption.Sulfhydryl reagents, bathophenanthroline, phthalein acids andazaserine produced strong inhibition of the enzyme activity.
1Permanent address: Department of Agricultural Chemistry, KyotoUniversity, Kyoto 606, Japan.
2To whom inquiries should be addressed. (Received July 7, 1979; ) 相似文献
44.
Toru Shimizu Masahiro Hatano Seiji Nagao Yoshinori Nozawa 《Biochemical and biophysical research communications》1982,106(4):1112-1118
43Ca NMR spectra of Ca2+-Tetrahymena calmodulin(Tet. CaM.) complexes have been observed under various conditions. Off-rate of Ca2+ from Tet. CaM. is estimated to be approx. 2.7 × 103 s?1 under a certain assumption. Relaxation rates of 43Ca NMR of Ca2+-Tet. CaM. are remarkably increased(by one order in magnitude) by adding trifluoperazine(TFP), a potent calmodulin antagonist. Relaxation parameters estimated suggest that Ca2+ mobility is reduced by the TFP binding. A stoichiometry of TFP is two moles per Tet. CaM. molecule. The relaxation rates of 43Ca NMR signals are increased by adding excessive Mg2+ to the Ca2+-Tet. CaM. solutions. The addition of Mg2+ to the Ca2+-Tet. CaM. complex decreases apparent pKa value of the complex as well. 相似文献
45.
K Imai M Maeda H Fujiwara N Okamoto M Kariya N Emi K Takakura H Kanzaki T Mori 《Biology of reproduction》1992,46(3):328-334
With specific monoclonal antibodies, we found that human endometrial stromal cells and decidual cells express two function-related surface antigens. Indirect immunofluorescence staining revealed that both endometrial stromal cells and decidual cells during the first trimester of pregnancy expressed cluster of differentiation (CD) 13 antigen and CD10 antigen, which are identical to aminopeptidase N and neutral endopeptidase, respectively. By flow cytometric analysis, CD13 antigen was detected on 82-93% of the examined cells, and CD10 antigen was detected on 75-93% of the examined cells in endometrial stromal cell-enriched preparations. Furthermore, peptidase activity was detected in these cell preparations by an assay based on the hydrolysis of alanine-p-nitroanilide into p-nitroaniline and alanine. 相似文献
46.
We have demonstrated for the first time that the sigma antagonist BMY 14802 prevents the development of behavioral sensitization induced by repeated administration of methamphetamine. Rats received an intraperitoneal injection of 15 or 30 mg/kg BMY 14802 followed by 2 mg/kg methamphetamine 30 min later. Unlike dopamine antagonists, BMY 14802 did not induce major changes in the acute motor effects of 2 mg/kg methamphetamine. Repeated administration of methamphetamine induced progressive augmentation of stereotyped behaviors and resulted in behavioral sensitization. However, repeated administration of methamphetamine in combination with BMY 14802 at either dose produced no increase in the intensity of stereotypy when compared with the first treatment. After a 7-day abstinence period, a challenge test with methamphetamine alone revealed supersensitivity of methamphetamine-sensitized rats to subsequent methamphetamine, whereas rats pretreated with repeated methamphetamine in combination with BMY 14802 exhibited no difference in the intensity of stereotypy from rats pretreated with repeated saline. These results suggest that sigma receptors play a crucial role in the induction of methamphetamine-induced sensitization. 相似文献
47.
48.
Kunshan Gao Yusho Aruga Kozi Asada Toshiaki Ishihara Toru Akano Masataka Kiyohara 《Journal of applied phycology》1991,3(4):355-362
Leafy thalli of the red algaPorphyra yezoensis Ueda, initiated from conchospores released from free-living conchocelis, were cultured using aeration with high CO2. It was found that the higher the CO2 concentration, the faster the growth of the thalli. Aeration with elevated CO2 lowered pH in dark, but raised pH remarkably in light with the thalli, because the photosynthetic conversion of HCO 3 ? to OH? and CO2 proceeded much faster than the dissociation of hydrated CO2 releasing H+. Photosynthesis of the alga was found to be enhanced in the seawater of elevated dissolved inorganic carbon (DIC, CO2 + HCO 3 ? + CO 3 ? ). It is concluded that the increased pH in the light resulted in the increase of DIC in the culture media, thus enhancing photosynthesis and growth. The relevance of the results to removal of atmospheric CO2 by marine algae is discussed. 相似文献
49.
Purification and characterization of hydroxypyruvate reductase from a serine-producing methylotroph, Hyphomicrobium methylovorum GM2 总被引:2,自引:0,他引:2
Y Izumi T Yoshida H Kanzaki S Toki S S Miyazaki H Yamada 《European journal of biochemistry》1990,190(2):279-284
Hydroxypyruvate reductase of a serine-producing methylotroph, Hyphomicrobium methylovorum GM2, was purified to complete homogeneity, crystallized and characterized, the first time for an enzyme from a methylotroph. The enzyme was found to be a dimer composed of identical subunits (38 kDa), the molecular mass of the enzyme being about 70 kDa. The enzyme was stable against heating at 25 degrees C for 10 min at pH values between 5 and 9. Optimal activity was observed at pH 6.8 and around 45 degrees C. The enzyme catalyzed the reduction of hydroxypyruvate with the oxidation of only NADH. Other than hydroxypyruvate, only glyoxylate served as a substrate. The Km values were found to be 0.175 mM for hydroxypyruvate and 10.8 mM for glyoxylate. Taking advantage of the high substrate specificity of this enzyme, a means of enzymatic determination of hydroxypyruvate was established. 相似文献
50.
Y Hirabayashi Y Matsumoto M Matsumoto T Toida N Iida T Matsubara T Kanzaki M Yokota I Ishizuka 《The Journal of biological chemistry》1990,265(3):1693-1701
Four major sialo compounds, termed GP-M1, GP-D1, GP-D2, and GP-D3 have been isolated from the urine of a novel glycoprotein storage disorder patient with angiokeratoma corporis diffusum which was discovered by Kanzaki et al. (Kanzaki, T., Yokota, M., Mizuno, N., Matsumoto, Y., and Hirabayashi, Y. (1989) Lancet April 22, 875-877). Based on the results of fast atom bombardment mass spectrometry, methylation analysis, and proton nuclear magnetic resonance spectroscopy, their chemical structures were concluded to be: (formula; see text) The yields of GP-M1, GP-D1, GP-D2, and GP-D3 were approximately 15, 6, 50, and 5 mg/liter of urine, respectively. The most major compound GP-D2, was further purified into single molecular species, threonine and serine type, by reversed phase high performance liquid chromatography. NMR analysis of the two purified compounds with single molecular species showed that the chemical shifts of anomeric protons of GalNAc were significantly different between threonine- and serine-linked GalNAc. Neither mannose-containing glycopeptides nor glycosphingolipids were excreted in the patient urine. From these results, this disease is thought to be caused by the deficiency of a lysosomal enzyme(s) acting on O-linked glycan chains. 相似文献