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51.
Kanwal Haneef Nadia Naeem Irfan Khan Hana’a Iqbal Nurul Kabir Siddiqua Jamall Muniza Zahid Asmat Salim 《Molecular biology reports》2014,41(5):3099-3112
Mesenchymal stem cells (MSCs) show accelerated regeneration potential when these cells experience hypoxic stress. This “preconditioning” has shown promising results with respect to cardio-protection as it stimulates endogenous mechanisms resulting in multiple cellular responses. The current study was carried out to analyze the effect of hypoxia on the expression of certain growth factors in rat MSCs and cardiomyocytes (CMs). Both cell types were cultured and assessed separately for their responsiveness to hypoxia by an optimized dose of 2,4,-dinitrophenol (DNP). These cells were allowed to propagate under normal condition for either 2 or 24 h and then analyzed for the expression of growth factors by RT-PCR. Variable patterns of expression were observed which indicate that their expression depends on the time of re-oxygenation and extent of hypoxia. To see whether the growth factors released during hypoxia affect the fusion of MSCs with CMs, we performed co-culture studies in normal and conditioned medium. The conditioned medium is defined as the medium in which CMs were grown for re-oxygenation till the specified time period of either 2 or 24 h after hypoxia induction. The results showed that the fusion efficiency of cells was increased when the conditioned medium was used as compared to that in the normal medium. This may be due to the presence of certain growth factors released by the cells under hypoxic condition that promote cell survival and enhance their fusion or regenerating ability. This study would serve as another attempt in designing a therapeutic strategy in which conditioned MSCs can be used for ischemic diseases and provide more specific therapy for cardiac regeneration. 相似文献
52.
Christopher R. Dunne Anthony R. Cillo Danielle R. Glick Katherine John Cody Johnson Jaspinder Kanwal Brian T. Malik Kristina Mammano Stefan Petrovic William Pfister Alexander S. Rascoe Diane Schrom Scott Shapiro Jeffrey W. Simkins David Strauss Rene Talai John P. Tomtishen III Josephine Vargas Tony Veloz Thomas O. Vogler Michael E. Clenshaw Devin T. Gordon-Hamm Kathryn L. Lee Elizabeth C. Marin 《PLoS biology》2014,12(12)
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Kanwal Ahmed Hideo Nemoto Toshiro Sugiyama Tadamichi Shimizu 《Chemico-biological interactions》2009,177(3):218-7685
The apoptosis-inducing ability of hybrid compounds composed of macrosphelide and thiazole-containing side chain of epothilones was investigated. Among the tested series of hybrid compounds the one containing thiazole side chain at C15 (MSt-2) showed the maximum potency to induce apoptosis, while another containing thiazole side chain at C3 (MSt-6) was less potent. MSt-2 was found to induce apoptosis in human lymphoma (U937) cells in a dose- and time-dependent manner as confirmed by DNA fragmentation analysis. MSt-2 treated cells showed rapid reactive oxygen species (ROS) formation and c-Jun N-terminal kinase (JNK) activation. Furthermore, significant activation of extrinsic pathway as evident by Fas expression and caspase-8 activation was also observed. MSt-2-mediated decreased expression of Bid is an important event for cross talk between intrinsic and extrinsic signaling. N-acetyl-l-cysteine pre-treatment rescued cells from MSt-2-induced ROS formation, mitochondrial membrane potential (Δψm) loss, Fas expression, caspase-8 and -3 activation and DNA fragmentation. Moreover, antioxidant enzymes catalase and/or superoxide dismutase conjugated with polyethylene glycol also inhibit MSt-2-induced ROS formation, apoptosis and Δψm loss suggesting thereby pro-oxidant effects of MSt-2. Furthermore, JNK and pan-caspase inhibitors also protect cells from MSt-2-induced apoptosis. In addition to this, MSt-2 was found to be more potent in human colon carcinoma (HCT116) and human gastric cancer (AGS) cells while it has no effect on human normal dermal fibroblast. The important structure-activity relationship observed in the current study which makes MSt-2 more potent than MSt-6 is the position of thiazole side chain and stereochemistry of position 3 in chemical structure. In short the results of our study demonstrate that MSt-2-induced rapid ROS generation and mitochondrial dysfunction in cells trigger events responsible for mitochondria-dependent apoptosis pathway. 相似文献
56.
Kanwal Ahmed Zheng-Li Wei Qing-Li Zhao Noriyuki Nakajima Takayuki Matsunaga Masaru Ogasawara Takashi Kondo 《Chemico-biological interactions》2010,185(3):182-188
The catechins, a family of polyphenols found in tea, can evoke various responses, including apoptosis. In this study we investigated whether the chemical modification of (?)-epigallocatechin gallate (EGCG) could enhance its apoptosis activity.We found that one of the catechin conjugated with capric acid [(2R,3S)-3′,4′,5,7-tetrahydroxyflavan-3-yl decanoate; catechin-C10] was most potent to induce apoptosis in U937 cells. C10 treatment resulted in a significant increase in reactive oxygen species (ROS) formation, mitochondrial membrane potential (MMP) loss, cytochrome c release caspase-9 and caspase-3 activation. In addition to this C10 also activated extrinsic pathway significantly as evident by time-dependent increase in Fas expression and caspase-8 activity. C10 mediated cleavage of Bid may be an important event for cross talk between intrinsic and extrinsic signaling. Moreover, pre-treatment of cells with anti-oxidant N-acetyl-l-cysteine (NAC) significantly prevented C10-induced apoptosis but did not protect MMP loss. Treatment of cells with pan-caspase inhibitor significantly inhibited apoptosis indicating that caspases are playing key role. In addition to this C10 was found to induce apoptosis in human colon cancer (HCT116) cells while it showed resistance to human keratinocytes (HaCat).In short our results showed that the optimal fatty acid side chain length is required for the apoptosis inducing activity of catechin derivatives in U937 cells. 相似文献
57.
Aroosa Kanwal Muhammad Bismillah Khan Mubshar Hussain Muhammad Naeem Muhammad Shahid Rizwan Muhammad Zafar-ul-Hye 《Phyton》2020,89(1):87-96
Worldwide, the dietary deficiency of zinc (Zn) is prevailing in almost
all arid and semi-arid regions. Zinc deficiency is not only the major constraint of
lower yield, but also dietary Zn deficiency in cereals grains may cause
increasing malnutrition and chronic health problems in human. Exogenous
application of Zn through basal soil nutrition might be a useful option to recover
Zn deficiency in mung bean. Therefore, field study was conducted to optimize
the optimum level and method of Zn nutrition to enhance crop yield and Zn
biofortification of mung bean through basal application. Zinc was applied at 0, 5,
10 and 15 kg/ha as basal application and side dressing, and in combination (50%
basal application + 50% side dressing). The results highlighted that Zn nutrition
prominently improved the mung bean yield as compared with control (no Zn
applied). The maximum grains yield and Zn concentration in grains were
obtained where Zn was applied at 15 kg/ha as basal application as compared
with all other combinations. Better improvement in grain yield was due to
significant increase in more number of pods and grain size owing to welldeveloped root system, improved leaf area index and high chlorophyll contents
in mung beans leaves. Amongst all applied Zn nutrition’s the basal application
of Zn (15 kg/ha) was a viable option to get higher yield and Zn biofortification
of mung bean. 相似文献
58.
The synthetic glycopeptides are interesting model systems to study the effect of O-glycosylation in modulating their function and structure. A series of glycosylated analogs of two antibacterial peptides, formaecin I and drosocin, were synthesized by varying the nature of sugar and its linkage with bioactive peptides to understand the influence of structure variation of glycosylation on their antibacterial activities. Higher antibacterial activities of all glycopeptides compared to their respective non-glycosylated counterparts emphasize in part the importance of sugar moieties in functional implications of these peptides. The consequences of the unique differences among the analogs were apparent on their antibacterial activities but not evident structurally by circular dichroism studies. We have shown that differently glycosylated peptides exhibit differential effect among each other when tested against several Gram-negative bacterial strains. The change of monosaccharide moiety and/or its anomeric configuration in formaecin I and drosocin resulted into decrease in the antibacterial activity in comparison to that of the native glycopeptide, but the extent of decrease in antibacterial activity of glycosylated drosocin analogs was less. Probably, the variation in peptide conformation arising due to topological dissimilarities among different sugars in the same peptide resulting in possible modulation in binding properties appears to be responsible for differences in their antibacterial activities. Indeed, these effects of glycosylation are found to be sequence-specific and depend in the milieu of amino acid residues. Interestingly, none of the carbohydrate variants affected the basic property of these peptides, which is non-hemolytic and non-toxicity to eukaryotic cells. 相似文献
59.
The molecular diversity of thirty-two different Morchella cultures/fruiting bodies, collected from the Western Himalayan region was studied in this investigation. Considerable taxonomic
confusion exists regarding many species of Morchella. Although classical taxonomy is helpful in identification for many ascomycetes, morels exhibit considerable morphological
diversity and there is disagreement in the identification of morel species. Phylogenetic analyses based on DNA sequences could
help in sorting out morel taxonomy which is essential to better define the morel diversity. In this study, sequence analysis
revealed that in the Western Himalayan region of India, both yellow (M. crassipes, M. spongiola) and black morels (M. elata, M. angusticeps, and M. gigas) were prominent along with two Verpa species. Phylogenetic analysis by maximum parsimony, maximum likelihood and Bayesian inference revealed two different clades
and a clear distinction between yellow and black morels. 相似文献
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