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排序方式: 共有75条查询结果,搜索用时 15 毫秒
31.
Toshiharu Oba Chieko Kurono Ritsuko Nakajima Tetsuo Takaishi Kazuto Ishida Geraldine A Fuller Wuthichai Klomkleaw Mamoru Yamaguchi 《Journal of applied physiology》2002,93(6):1999-2008
We studied whether hydrogen peroxide (H(2)O(2)) at =10 microM activates the ryanodine receptor and decreases releasable Ca(2+) content in the sarcoplasmic reticulum after fatigue. Exposure of rabbit or frog skeletal muscle ryanodine receptors to 10 microM H(2)O(2) enhanced channel activity in lipid bilayers when the redox potential was defined at cis = -220 mV and trans = -180 mV. Channel activation by 10 microM H(2)O(2) was also observed when cis potential was set at -220 mV without defining trans potential, but the effect was less. Reduction of trans redox potential from -180 to -220 mV did not alter channel activity. H(2)O(2) at 500 microM failed to activate the channel when the redox potential was not controlled. Stimulation of the frog muscle fiber for 2 min (50 Hz, a duty cycle of 200 ms/s) decreased tetanus tension by approximately 50%. After 1 min, tetanus recovered rapidly to approximately 70% of control and thereafter slowly approached the control level. Amplitudes of caffeine- and 4-chloro-m-cresol-induced contractures were decreased after a 60-min rest. The decrease is not enhanced by exposure to 10 microM H(2)O(2). These results suggest that H(2)O(2) markedly activates the ryanodine receptor under the redox control in vitro, but externally applied H(2)O(2) may not play an important role in the postfatigue recovery process. 相似文献
32.
E xcept for the crystalline deposits of hypoxanthine found in skeletal muscle of patients with congenital xanthine oxidase deficiency (xanthinuria) there are few reports concerning oxypurines in the mammalian tissues (P arker , S nedden and W atts , 1969, 1970). Since it is difficult to separate hypoxanthine from xanthine in biological fluids (S immonds and W ilson , 1967), the distribution of hypoxanthine in mammalian tissues is not known in detail.
This paper shows that cubic or rod crystals of C5 H4 ON4 are easily isolated from calf brain by column chromatography with ion exchange resin Amberlite CG120 and their identity with hypoxanthine was shown by means of nuclear magnetic resonance and mass Spectrometry. 相似文献
This paper shows that cubic or rod crystals of C
33.
Y Shimohigashi T Ogasawara T Koshizaka M Waki T Kato N Izumiya M Kurono K Yagi 《Biochemical and biophysical research communications》1987,146(3):1109-1115
Dimeric analogues of the inactive enkephalin fragment Tyr-D-Ala-Gly were synthesized by cross-linking with alkanediamine at the C-terminus. Biological evaluation of these dimers (H-Tyr-D-Ala-Gly-NH)2.(-CH2-)n (DTREn), where n = 0-6, revealed that the fragment inactive for mu receptors was activated by its dimerization, with the maximum activation found with DTRE2, and that the dimer was highly mu-selective. So-called "handicapped" dimers, which lack one of the essential groupings required for enkephalin activity, were found to be far less active, indicating that the dimer interacts bivalently with mu receptors. It seems, therefore, that mu opiate receptors contain at least two equivalent binding sites which are extremely close to each other. 相似文献
34.
Kurien BT Patel NC Porter AC Kurono S Matsumoto H Wang H Scofield RH 《Analytical biochemistry》2004,331(2):224-229
Proline-containing peptides of the X-proline type are cleaved by the dipeptidase prolidase. The classical method of prolidase assay relied on the colorimetric estimation of the liberated proline with ninhydrin using acidic media and heat. This method, however, gave inconsistent results due to the nonspecificity of the ninhydrin color reaction. We report here a method for the detection of the liberated proline using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. Human sera were incubated with a mixture containing the dipeptide glycyl-proline in Tris-HCl supplemented with manganese at 37 degrees C for 24h. The samples were precipitated with trifluoroacetic acid and centrifuged. An aliquot of the supernatant was mixed with an equal volume of ferulic acid solution. An aliquot from this mixture was spotted on a stainless steel mass spectrometry grid and analyzed using MALDI-TOF mass spectrometry. The activity of the enzyme was determined by the complete disappearance of the glycyl-proline peak with the concomitant appearance of the proline peak and can be expressed in terms of the ratio of the area beneath the proline to the area beneath the glycyl-proline peak. Subjects homozygous for prolidase deficiency had a ratio ranging from 0.006 to 0.04 while obligatory heterozygotes had a ratio ranging from around 1.1 to 2.4. Normal subjects had ratios ranging from 9 to 239. Using this method we have unambiguously identified subjects with homozygous or heterozygous prolidase deficiency. In addition to the advantage of rapid sample preparation time, this method is highly specific, reproducible, and sensitive. 相似文献
35.
Fumitoshi Irie Sadamu Kurono Yu-Teh Li Yousuke Seyama Yoshio Hirabayashi 《Glycoconjugate journal》1996,13(2):177-186
In the present study, three extremely minor but novel Chol-1 antigens, termed X1, X2, and X3 have been isolated from bovine brain gangliosides. Based on the results of sialidase degradation, TLC-immunostaining with anti-Chol-1 antibody and fast atom bombardment mass spectrometry, their chemical structures were identified as: $$\begin{gathered} III^6 NeuAc--GgOse4Cer (X1:GM1\alpha ) \hfill \\ III^6 NeuAc,II^3 NeuAc--GgOse4Cer (X2:GT1a\alpha ) \hfill \\ III^6 NeuAc,II^3 NeuAc--NeuGc--GgOse4Cer (X3:GT1b\alpha ) \hfill \\ \end{gathered} $$ The yields of GM1α, GD1aα, and GT1bα, were approximately 150, 20, and 10 µg, respectively, from 10 g of the bovine brain ganglioside mixture. In conjunction with our previous observations, all gangliosides with anti-Chol-1 reactivity were found to contain a common sialyl α2–6N-acetylgalactosamine residue, indicating that this unique sialyl linkage is the specific antigenic determinant. We subsequently examined the biosyntheses of the three novel Chol-1 gangliosides using rat liver Golgi fraction as an enzyme source. The results showed that GM1α, GD1aα, and GT1bα were synthesized from asialo-GM1, GM1a, and GD1b, respectively, by the action of a GalNAc α2-6sialyltransferase. 相似文献
36.
Y Shimohigashi T Ogasawara H Kodama T Koshizaka M Kurono K Yagi 《Biochemistry international》1989,18(6):1107-1110
A series of analogs of a dimeric peptide of the inactive fragment of enkephalin (H-Tyr-D-Ala-Gly-NH-CH2-)2 (DTRE2) was synthesized by modifying one or both of tyrosine residues. The change of configuration of both tyrosines, from L to D, or the removal of both p-hydroxy groups brought about a decrease in activity, but some of the activity (about 20% of the parent enkephalin dimer's activity) was found to be retained when assayed by using guinea pig ileum and mouse vas deferens. In contrast, the analog lacking amino groups in both tyrosines was completely devoid of activity, indicating the essential importance of the tyrosine amino group in opiate receptor recognition. The activity of analogs with only one amino group was found to be higher than that of the parent enkephalin dimer having both amino groups. A possible interaction to explain this finding was discussed. 相似文献
37.
38.
Akio Yamazaki Vladimir A. Bondarenko Isao Matsuura Masahiro Tatsumi Sadamu Kurono Naoka Komori Hiroyuki Matsumoto Fumio Hayashi Russell K. Yamazaki Jiro Usukura 《Molecular and cellular biochemistry》2010,339(1-2):215-233
Cyclic GMP phosphodiesterase (PDE) in bovine rod photoreceptor outer segments (OS) comprises a catalytic subunit complex (Pαβ) and two inhibitory subunits (Pγ) and is regulated by the α subunit of transducin (Tα). Here, we show an overall mechanism for PDE regulation by identifying Pγ complexes in OS homogenates prepared with an isotonic buffer. Before Tα activation, three Pγ complexes exist in the soluble fraction. Complex a, a minor complex, contains Pαβ, Tα, and a protein named Pδ. Complex b, Pαβγγ b , has a PDE activity similar to that of membranous Pαβγγ, Pαβγγ M , and its level, although its large portion is Pδ-free, is estimated to be 20–30% of the total Pαβγγ. Complex c, (Pγ·GDP-Tα) 2 c , appears to be a dimer of Pγ·GDP-Tα. Upon Tα activation, (1) complex a stays unchanged, (2) Pαβγγ b binds to membranes, (3) the level of (Pγ·GDP-Tα) 2 c is reduced as its GTP-form is produced, (4) complex d, Pγ·GTP-Tα d , is formed on membranes and its substantial amount is released to the soluble fraction, and (5) membranous Pαβγγ, Pαβγγ M and/or Pαβγγ b , becomes Pγ-depleted. These observations indicate that Pγ as a complex with GTP-Tα dissociates from Pαβγγ on membranes and is released to the soluble fraction and that Pγ-depleted PDE is the GTP-Tα-activated PDE. After GTP hydrolysis, both (Pγ·GDP-Tα) 2 c and Pγ·GDP-Tα d , without liberating Pγ, deactivate Pγ-depleted PDE. The preferential order to be used for the deactivation is membranous Pγ·GDP-Tα d , solubilized Pγ·GDP-Tα d and (Pγ·GDP-Tα) 2 c . Release of Pγ·GTP-Tα complexes to the soluble fraction is relevant to light adaptation. 相似文献
39.
Takashi Wakabayashi Chieko Kurono Masahisa Asano Hiroshi Kimura Takayuki Ozawa 《Journal of bioenergetics and biomembranes》1976,8(1):55-71
Microsomes were obtained from the zona glomerulosa of the bovine adrenal cortex. Contamination of microsomes with other cellular organelles was examined using various marker enzymes and the electron microscope. Distribution of cytochrome P-450 in the zona glomerulosa was studied using various fractions including microsomes, described above, and mitochondria. The amount of cytochrome P-450 in mitochondria and in microsomes was determined to be 0.73 and 0.32 nmol/mg protein, respectively. The CO difference spectrum was affected not only by the concentration of added deoxycholate but also by the incubation time after addition. Approximately 40–50% of cytochrome P-450 in the samples was converted to cytochrome P-420 within 20–30 sec of incubation with deoxycholate.The content of RNA, phospholipids, and cytochromeb
5 in microsomes obtained from the zona glomerulosa is also evaluated in comparison to that in microsomes obtained from the zona fasciculoreticularis. 相似文献
40.
Kurata H Kusumi K Otsuki K Suzuki R Kurono M Takada Y Shioya H Komiya T Mizuno H Ono T Hagiya H Minami M Nakade S Habashita H 《Bioorganic & medicinal chemistry letters》2011,21(13):3885-3889
Structure-activity relationship of sphingosine-1-phosphate receptor agonists was examined. Cinnamyl derivative 1 was modified to improve S1P1 agonistic activity as well as selectivity over S1P3 agonistic activity. Dihydronaphthalene derivative 10d was identified as a potent S1P1 receptor agonist with high selectivity against S1P3 and enhanced efficacy in lowering peripheral lymphocyte counts in mice. 相似文献