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31.
Hong-Ming Cheng Morifusa Eto Kanji Nakamura Shozo Kuwatsuka Yasuyoshi Oshima Masaru Kado 《Bioscience, biotechnology, and biochemistry》2013,77(9):1162-1174
More than ninety phenylphenol derivatives were tested for fungistatic activity toward ten species of agriculturally important fungi. One of N-methylcarbamates and some nitro- or chloro-substituted derivatives were highly toxic against Piricularia oryzae. From the relationship between the chemical structure and the activity, it is supposed that the reactivity, the permeability, the steric effect and the metabolic activation of the compound are the responsible factors for the fungistatic activity. 相似文献
32.
Akikazu Hatanaka Tadahiko Kajiwara Takatoshi Koda 《Bioscience, biotechnology, and biochemistry》2013,77(10):2115-2117
The substrate specificity of enzyme system producing C6-aldehyde in Thea chloroplasts was clarified with an entire series of synthesized positional isomers, in which the position of cis-1, cis-4-pentadiene system varies from C-3 to C-13 in C18 fatty acid and geometrical isomers of linoleic acid. The structural requirement for the substrate of enzyme system producing C6-aldehyde is the presence of cis-1, cis-4-pentadiene system between ω-6 and ω-10. 相似文献
33.
34.
Jiro Sekiya Tadahiko Kajiwara Akikazu Hatanaka 《Bioscience, biotechnology, and biochemistry》2013,77(3):677-678
A simple electronic device was constructed which, in combination with a conventional titrator, records close approximation of the buffer capacity curve (β-pH curve) for the solution of unknown composition. Since the recorded curves provide the overall picture of the distribution of weak Brönsted acids in the solution on the pKa axis, this apparatus may be useful in various fields such as chemistry of food and agricultural products and clinical medicine, where the characterization of the complex mixtures of weak electrolytes — carboxylic acids, amono acids, proteins, amines, phenols and etc. — are important. 相似文献
35.
Jiro Sekiya Wataru Kawasaki Tadahiko Kajiwara Akikazu Hatanaka 《Bioscience, biotechnology, and biochemistry》2013,77(8):1677-1678
d-Coronamic acid was deaminated by 1-aminocyclopropane-1-carboxylate (ACPC) deaminase to produce α-keto-n-caproic acid. This deaminase which was purified from Pseudomonas sp. ACP was active to only d-coronamic acid among its stereoisomers. l-Coronamic acid or dl-allocoronamic acid was inactive or negligibly poor as the substrate. In addition, both deamination of ACPC and d-coronamic acid were inhibited by l-alanine, not by d-isomer and the inhibition of ACPC deamination by l-alanine was competitive. On the basis of these results, stereoselectivity of the enzymatic deamination was discussed. 相似文献
36.
Tadahiko Kajiwara Yoshinobu Odake Akikazu Hatanaka 《Bioscience, biotechnology, and biochemistry》2013,77(8):1617-1621
3Z-Nonenal and 3Z, 6Z-nonadienal, potential biosynthetic precursors of 2E-nonenal and 2E, 6Z-nonadienal, were for the first time synthesized stereoseleclively. 相似文献
37.
Jiro Sekiya Shinji Numa Tadahiko Kajiwara Akikazu Hatanaka 《Bioscience, biotechnology, and biochemistry》2013,77(1):185-190
The synthetic activity for 3Z-hexenal, an important precursor of 3Z-hexenol (leaf alcohol), was localized in chloroplasts of Thea sinensis leaves. 3Z-Hexenal, which is easily isomerized to 2E-hexenal (leaf aldehyde), was formed from linolenic acid in the presence of oxygen. 13-l-Hydroperoxy-linolenic acid also served as a precursor, but the triglyceride and methyl ester of linolenic acid did not. This enzyme system appeared to be tightly bound to the lamellae membranes of chloroplasts. 相似文献
38.
Sirida Youngchim Joshua D. Nosanchuk Soraya Pornsuwan Susumu Kajiwara Nongnuch Vanittanakom 《PloS one》2013,8(6)
Melanins are synthesized by organisms of all biological kingdoms and comprise a heterogeneous class of natural pigments. Certain of these polymers have been implicated in the pathogenesis of several important human fungal pathogens. This study investigated whether the fungal skin pathogen Malassezia furfur produces melanin or melanin-like compounds. A melanin-binding monoclonal antibody (MAb) labelled in vitro cultivated yeast cells of M. furfur. In addition, melanization of Malassezia yeasts and hyphae was detected by anti-melanin MAb in scrapings from patients with pityriasis versicolor. Treatment of Malassezia yeasts with proteolytic enzymes, denaturant and concentrated hot acid yielded dark particles and electron spin resonance spectroscopy revealed that these particles contained a stable free radical compound, consistent with their identification as melanins. Malassezia yeasts required phenolic compounds, such as L-DOPA, in order to synthesize melanin. L-DOPA also triggered hyphal formation in vitro when combined with kojic acid, a tyrosinase inhibitor, in a dose-dependent manner. In this respect, L-DOPA is thought to be an essential substance that is linked to both melanization and yeast-mycelial transformation in M. furfur. In summary, M. furfur can produce melanin or melanin-like compounds in vitro and in vivo, and the DOPA melanin pathway is involved in cell wall melanization. 相似文献
39.
Yukiko Tomioka Yoshikazu Fujimoto Kanji Nakai Kinuyo Ozaki Sayo Yamamoto Haruka Suyama Masami Morimatsu Toshihiro Ito Etsuro Ono 《Biochemistry and Biophysics Reports》2016
Transgenic mouse lines expressing a soluble form of human nectin-2 (hNectin-2Ig Tg) exhibited distinctive elevation of amylase and lipase levels in the sera. In this study, we aimed to clarify the histopathology and to propose the transgenic mouse lines as new animal model for characteristic pancreatic exocrine defects. The significant increase of amylase and lipase levels in sera of the transgenic lines approximately peaked at 8 weeks old and thereafter, plateaued or gradually decreased. The histopathology in transgenic acinar cells was characterized by intracytoplasmic accumulation of abnormal proteins with decrease of normal zymogen granules. The hNectin-2Ig expression was observed in the cytoplasm of pancreatic acinar cells, which was consistent with zymogen granules. However, signals of hNectin-2Ig were very weak in the transgenic acinar cells with the abnormal cytoplasmic accumulaion. The PCNA-positive cells increased in the transgenic pancreas, which suggested the affected acinar cells were regenerated. Acinar cells of hNectin-2Ig Tg had markedly small number of zymogen granules with remarkable dilation of the endoplasmic reticulum (ER) lumen containing abundant abnormal proteins. In conclusion, hNectin-2Ig Tg is proposed as a new animal model for characteristic pancreatic exocrine defects, which are due to the ER stress induced by expression of mutated cell adhesion molecule that is a soluble form of human nectin-2. 相似文献