Floristic diversity and co-occurrences in a subtropical broad-leaved forest and two contrasting regrowth stands in central-west Yunnan Province,China

Many of the natural forested ecosystems that still remain in mainland China are being cleared with potentially detrimental effects on woody plant species diversity on both local and regional scales. The most extensive stand of subtropical broad-leaved forest remaining in China is located in Yunnan Province. In an effort to document the influence of human-induced disturbance on Yunnan's woody flora, floristic inventories were conducted in a stand of primary forest and in regrowth stands located in its interior and along its outer margin in the Xujiaba Nature Sanctuary in the Ailao Mountain Range. Of particular interest was the location of the disturbance relative to the primary forest source area. A total of 134 woody plant species representing 74 genera and 43 families were recorded. The floristics of the two regrowth stands were significantly different from each other, with < 10% of their respective floras comprised of co-occurring species. The interior regrowth stand had a higher number of co-occurring species with the primary forest; however, > 40% were still non-co-occurring.The principal families represented in the primary forest and the interior regrowth stand were Aquifoliaceae, Berberidaceae, Fagaceae, Lauraceae, Rosaceae, Smilacaceae, Symplocaceae, Theaceae, and Vacciniaceae. The three dominant species with relative importance values ranging from > 5% to 18% in both the primary forest and the interior regrowth stand were Castanopsis wattii, Lithocarpus jingdongensis, and Symplocos sumuntia. The edge regrowth stands had the lowest species diversity and were dominated by the native pine Pinus yunnanensis, with a relative importance of 24%. The principal families represented in the edge regrowth stand were Betulaceae, Ericaceae, Fagaceae, Myricaceae, Pinaceae, and Theaceae. Only the Fagaceae and Theaceae were well-represented in all three stands. The results of the study document the low species diversity in post-cutting regrowth on the margins of the primary forest as compared with post-cutting regrowth in the forest interior.     

RAPD typing of clinical isolates of Staphylococcus haemolyticus

The randomly amplified polymorphic DNA (RAPD) assay was used to generate DNA fingerprints from clinical isolates of Staphylococcus haemolyticus isolated from patients treated with continuous ambulatory peritoneal dialysis and previously subjected to a combination of typing methods. The RAPD profiles generated with one of six randomly designed 10-mer primers allowed visual discrimination of strains. Good correlation with the original typing scheme was achieved but RAPD typing allowed discrimination of strains previously indistinguishable.     

Comparison of prevalence of schizophrenia among residents of hostels for homeless people in 1966 and 1992.

OBJECTIVE--To determine whether the prevalence of schizophrenia among the homeless population of Edinburgh resident in hostels has changed between 1966 and 1992. DESIGN--Comparison of two cross sectional surveys. SETTINGS--Hostels for homeless people in Edinburgh. SUBJECTS--In 1966 a random sample of 98 residents of three common lodging houses. In 1992 a random sample of 198 residents of nine hostels. MAIN OUTCOME MEASURE--Prevalence of schizophrenia. RESULTS--The prevalence of schizophrenia in 1992 was 12/136 (9%) compared with 20/79 (25%) in 1966 (odds ratio 0.29; 95% confidence interval 0.13 to 0.62; P = 0.001). Adjustment for confounding by age, current hostel, and duration of unemployment by means of logistic regression produced an adjusted odds ratio of 0.22 (0.08 to 0.58). CONCLUSIONS--The prevalence of schizophrenia was lower in 1992 even after other changes in the population resident in hostels occurring between 1966 and 1992 were taken into account. The findings are not consistent with an increase in the prevalence of schizophrenia among homeless people despite a 66% reduction in adult psychiatric beds in the region during 1966-92.     

中国盲走螨属一新种和二新纪录：蜱螨亚纲：植绥螨科

本文记述我国植绥螨科盲走螨属Ｔｙｐｈｌｏｄｒｏｍｕｓ一新种和中国二新纪录：鲁盲走螨Ｔ．ｌｕｅｎｓｉｓ ｓｐ．ｎｏｖ．,甲胄盲走螨Ｔ．ｌｏｒｃａｔｕｓ,肥厚盲走螨Ｔ．ｈｉｇｏｅｎｓｉｓ。标本保存于上海复旦大学环境和资源生物系。     

Production and initial characterisation of the xylan-degrading system of Phanerochaete chrysosporium

We report the optimum conditions for the degradation of oat spelt arabinoxylan and a preliminary characterisation of the inducible xylan-degrading system of the lignin-degrading white-rot fungus Phanerochaete chrysosporium. Xylanase activity was optimal at pH 5.0 and 50°C; see attached sheet the maximum reaction velocity (V_max) of the system was 3.86 units (U) mg^–1 protein with arabinoxylan as substrate and the substrate concentration giving half V_max (S_0.5) was 0.52 mg ml^–1. At concentrations of arabinoxylan greater than 15 mg ml^–1 excess substrate inhibition was observed. Xylose at 0.9 mm inhibited activity to the extent of 50%. Xylanase activity increased as a function of the dilution of the enzyme preparation prior to assay. It was resolved into four peaks by using a DEAE-Biogel column; the material in these peaks differed with respect to xylan solubilisation and the formation of reducing sugars. Electrofocusing gels allowed visualisation of several bands of activity corresponding to each peak. The arabinoxylan degradation system of P. chrysosporium is therefore composed of multiple components. Correspondence to: P. Broda     

Increased shikonin production by hairy roots of Lithospermum erythrorhizon in two phase bubble column reactor

Summary Plant hairy root cultures of Lithospermum erythrorhizon were carried out to produce shikonin derivatives by employing in situ extraction with n-hexadecane in a shake flask and a bubble column bioreactor. Over 95 % shikonin produced was recovered in the n-hexadecane layer. In flask cultures the maximum concentration of shikonin with n-hexadecane extraction was 3 times higher than that obtained without extraction. In the two phase bubble column reactor, 572.6 mg/L of shikonin and 15.6 g/L of dry cell mass were obtained after 54 days. Shikonin was produced at a constant level of 10.6 mg/L day during this period.     

钙／钙调素依赖性蛋白激酶对17．7kD和6kD胰腺蛋白的磷酸化

本文报导了胰腺提取物中两种可被钙／钙调素依赖性蛋白激酶磷酸化的热稳定蛋白。ＳＤＳ－ＰＡＧＥ测定其表观分子量分别为１７．７ｋＤ和６ｋＤ。经钙／钙调素依赖性蛋白激酶磷酸化后,其最大磷酸参入量为８．８μｍｏｌ／ｇ蛋白。同时磷酸化作用导致１７．７ｋＤ蛋白在ＳＤＳ－ＰＡＧＥ中迁移率发生变化。本文还进一步分析了各种阳离子对磷酸化的影响,并对此两种蛋白可能具有的生理功能进行了初步探讨。