排序方式: 共有643条查询结果,搜索用时 15 毫秒
51.
Crystal structure of Ufc1, the Ufm1-conjugating enzyme 总被引:2,自引:0,他引:2
Mizushima T Tatsumi K Ozaki Y Kawakami T Suzuki A Ogasahara K Komatsu M Kominami E Tanaka K Yamane T 《Biochemical and biophysical research communications》2007,362(4):1079-1084
Ubiquitin and ubiquitin-like protein-conjugating enzymes play central roles in posttranslational modification processes. The ubiquitin-fold modifier 1 (Ufm1), one of a variety of ubiquitin-like modifiers, is covalently attached to target proteins via Uba5 and Ufm1-conjugating enzyme 1 (Ufc1), which are analogous to the E1 and E2 ubiquitylation enzymes. As Ufm1-related proteins are conserved in metazoa and plants, the Ufm1 system likely plays important roles in various multicellular organisms. Herein, we report the X-ray structure of human Ufc1 determined at 1.6 A resolution. The Ufc1 structure comprises a canonical E2 domain and an additional N-terminal domain. The Uba5 binding site on Ufc1 was assigned by structural comparison of Ufc1 and Ubc12 and related mutational analyses. In addition, we show that the N-terminal unique domain of Ufc1 contributes to thermal stability. 相似文献
52.
Genetic and epigenetic alteration among three homoeologous genes of a class E MADS box gene in hexaploid wheat 总被引:4,自引:0,他引:4 下载免费PDF全文
Shitsukawa N Tahira C Kassai K Hirabayashi C Shimizu T Takumi S Mochida K Kawaura K Ogihara Y Murai K 《The Plant cell》2007,19(6):1723-1737
Bread wheat (Triticum aestivum) is a hexaploid species with A, B, and D ancestral genomes. Most bread wheat genes are present in the genome as triplicated homoeologous genes (homoeologs) derived from the ancestral species. Here, we report that both genetic and epigenetic alterations have occurred in the homoeologs of a wheat class E MADS box gene. Two class E genes are identified in wheat, wheat SEPALLATA (WSEP) and wheat LEAFY HULL STERILE1 (WLHS1), which are homologs of Os MADS45 and Os MADS1 in rice (Oryza sativa), respectively. The three wheat homoeologs of WSEP showed similar genomic structures and expression profiles. By contrast, the three homoeologs of WLHS1 showed genetic and epigenetic alterations. The A genome WLHS1 homoeolog (WLHS1-A) had a structural alteration that contained a large novel sequence in place of the K domain sequence. A yeast two-hybrid analysis and a transgenic experiment indicated that the WLHS1-A protein had no apparent function. The B and D genome homoeologs, WLHS1-B and WLHS1-D, respectively, had an intact MADS box gene structure, but WLHS1-B was predominantly silenced by cytosine methylation. Consequently, of the three WLHS1 homoeologs, only WLHS1-D functions in hexaploid wheat. This is a situation where three homoeologs are differentially regulated by genetic and epigenetic mechanisms. 相似文献
53.
54.
55.
Insecticide applications to soil contribute to the development of Burkholderia mediating insecticide resistance in stinkbugs 下载免费PDF全文
Kanako Tago Yoshitomo Kikuchi Sinji Nakaoka Chie Katsuyama Masahito Hayatsu 《Molecular ecology》2015,24(14):3766-3778
Some soil Burkholderia strains are capable of degrading the organophosphorus insecticide, fenitrothion, and establish symbiosis with stinkbugs, making the host insects fenitrothion‐resistant. However, the ecology of the symbiotic degrading Burkholderia adapting to fenitrothion in the free‐living environment is unknown. We hypothesized that fenitrothion applications affect the dynamics of fenitrothion‐degrading Burkholderia, thereby controlling the transmission of symbiotic degrading Burkholderia from the soil to stinkbugs. We investigated changes in the density and diversity of culturable Burkholderia (i.e. symbiotic and nonsymbiotic fenitrothion degraders and nondegraders) in fenitrothion‐treated soil using microcosms. During the incubation with five applications of pesticide, the density of the degraders increased from less than the detection limit to around 106/g of soil. The number of dominant species among the degraders declined with the increasing density of degraders; eventually, one species predominated. This process can be explained according to the competitive exclusion principle using Vmax and Km values for fenitrothion metabolism by the degraders. We performed a phylogenetic analysis of representative strains isolated from the microcosms and evaluated their ability to establish symbiosis with the stinkbug Riptortus pedestris. The strains that established symbiosis with R. pedestris were assigned to a cluster including symbionts commonly isolated from stinkbugs. The strains outside the cluster could not necessarily associate with the host. The degraders in the cluster predominated during the initial phase of degrader dynamics in the soil. Therefore, only a few applications of fenitrothion could allow symbiotic degraders to associate with their hosts and may cause the emergence of symbiont‐mediated insecticide resistance. 相似文献
56.
Takeshi Inagaki Satoshi Iwasaki Yoshihiro Matsumura Takeshi Kawamura Toshiya Tanaka Yohei Abe Ayumu Yamasaki Yuya Tsurutani Ayano Yoshida Yoko Chikaoka Kanako Nakamura Kenta Magoori Ryo Nakaki Timothy F. Osborne Kiyoko Fukami Hiroyuki Aburatani Tatsuhiko Kodama Juro Sakai 《The Journal of biological chemistry》2015,290(7):4163-4177
Polycomb repressive complex 1 (PRC1) plays an essential role in the epigenetic repression of gene expression during development and cellular differentiation via multiple effector mechanisms, including ubiquitination of H2A and chromatin compaction. However, whether it regulates the stepwise progression of adipogenesis is unknown. Here, we show that FBXL10/KDM2B is an anti-adipogenic factor that is up-regulated during the early phase of 3T3-L1 preadipocyte differentiation and in adipose tissue in a diet-induced model of obesity. Interestingly, inhibition of adipogenesis does not require the JmjC demethylase domain of FBXL10, but it does require the F-box and leucine-rich repeat domains, which we show recruit a noncanonical polycomb repressive complex 1 (PRC1) containing RING1B, SKP1, PCGF1, and BCOR. Knockdown of either RING1B or SKP1 prevented FBXL10-mediated repression of 3T3-L1 preadipocyte differentiation indicating that PRC1 formation mediates the inhibitory effect of FBXL10 on adipogenesis. Using ChIP-seq, we show that FBXL10 recruits RING1B to key specific genomic loci surrounding the key cell cycle and the adipogenic genes Cdk1, Uhrf1, Pparg1, and Pparg2 to repress adipogenesis. These results suggest that FBXL10 represses adipogenesis by targeting a noncanonical PRC1 complex to repress key genes (e.g. Pparg) that control conversion of pluripotent cells into the adipogenic lineage. 相似文献
57.
58.
Hikaru Tsukazaki Shigenori Yaguchi Shusei Sato Hideki Hirakawa Yuichi Katayose Hiroyuki Kanamori Kanako Kurita Takeshi Itoh Masahiko Kumagai Satoshi Mizuno Masao Hamada Hiroyuki Fukuoka Ken-ichiro Yamashita John A. McCallum Masayoshi Shigyo Tadayuki Wako 《Molecular breeding : new strategies in plant improvement》2015,35(1):1-11
59.
60.
Kanako Sugahara Yuko Kaneko Satoshi Ito Keisuke Yamanaka Hitoshi Sakio Kazuhiko Hoshizaki Wajiro Suzuki Norikazu Yamanaka Hiroaki Setoguchi 《Journal of plant research》2011,124(1):75-83
Japanese horse chestnut (Aesculus turbinata: Hippocastanaceae) is one of the typical woody plants that grow in temperate riparian forests in the Japanese Archipelago.
To analyze the phylogeography of this plant in the Japanese Archipelago, we determined cpDNA haplotypes for 337 samples from
55 populations covering the entire distribution range. Based on 1,313 bp of two spacers, we determined ten haplotypes that
are distinguished from adjacent haplotypes by one or two steps. Most of the populations had a single haplotype, suggesting
low diversity. Spatial analysis of molecular variance suggested three obvious phylogeographic structures in western Japan,
where Japanese horse chestnut is scattered and isolated in mountainous areas. Conversely, no clear phylogeographic structure
was observed from the northern to the southern limit of this species, including eastern Japan, where this plant is more common.
Rare and private haplotypes were also found in southwestern Japan, where Japanese horse chestnuts are distributed sparsely.
These findings imply that western Japan might have maintained a relatively large habitat for A. turbinata during the Quaternary climatic oscillations, while northerly regions could not. 相似文献