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Insecticide applications to soil contribute to the development of Burkholderia mediating insecticide resistance in stinkbugs
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Kanako Tago Yoshitomo Kikuchi Sinji Nakaoka Chie Katsuyama Masahito Hayatsu 《Molecular ecology》2015,24(14):3766-3778
Some soil Burkholderia strains are capable of degrading the organophosphorus insecticide, fenitrothion, and establish symbiosis with stinkbugs, making the host insects fenitrothion‐resistant. However, the ecology of the symbiotic degrading Burkholderia adapting to fenitrothion in the free‐living environment is unknown. We hypothesized that fenitrothion applications affect the dynamics of fenitrothion‐degrading Burkholderia, thereby controlling the transmission of symbiotic degrading Burkholderia from the soil to stinkbugs. We investigated changes in the density and diversity of culturable Burkholderia (i.e. symbiotic and nonsymbiotic fenitrothion degraders and nondegraders) in fenitrothion‐treated soil using microcosms. During the incubation with five applications of pesticide, the density of the degraders increased from less than the detection limit to around 106/g of soil. The number of dominant species among the degraders declined with the increasing density of degraders; eventually, one species predominated. This process can be explained according to the competitive exclusion principle using Vmax and Km values for fenitrothion metabolism by the degraders. We performed a phylogenetic analysis of representative strains isolated from the microcosms and evaluated their ability to establish symbiosis with the stinkbug Riptortus pedestris. The strains that established symbiosis with R. pedestris were assigned to a cluster including symbionts commonly isolated from stinkbugs. The strains outside the cluster could not necessarily associate with the host. The degraders in the cluster predominated during the initial phase of degrader dynamics in the soil. Therefore, only a few applications of fenitrothion could allow symbiotic degraders to associate with their hosts and may cause the emergence of symbiont‐mediated insecticide resistance. 相似文献
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Takeshi Inagaki Satoshi Iwasaki Yoshihiro Matsumura Takeshi Kawamura Toshiya Tanaka Yohei Abe Ayumu Yamasaki Yuya Tsurutani Ayano Yoshida Yoko Chikaoka Kanako Nakamura Kenta Magoori Ryo Nakaki Timothy F. Osborne Kiyoko Fukami Hiroyuki Aburatani Tatsuhiko Kodama Juro Sakai 《The Journal of biological chemistry》2015,290(7):4163-4177
Polycomb repressive complex 1 (PRC1) plays an essential role in the epigenetic repression of gene expression during development and cellular differentiation via multiple effector mechanisms, including ubiquitination of H2A and chromatin compaction. However, whether it regulates the stepwise progression of adipogenesis is unknown. Here, we show that FBXL10/KDM2B is an anti-adipogenic factor that is up-regulated during the early phase of 3T3-L1 preadipocyte differentiation and in adipose tissue in a diet-induced model of obesity. Interestingly, inhibition of adipogenesis does not require the JmjC demethylase domain of FBXL10, but it does require the F-box and leucine-rich repeat domains, which we show recruit a noncanonical polycomb repressive complex 1 (PRC1) containing RING1B, SKP1, PCGF1, and BCOR. Knockdown of either RING1B or SKP1 prevented FBXL10-mediated repression of 3T3-L1 preadipocyte differentiation indicating that PRC1 formation mediates the inhibitory effect of FBXL10 on adipogenesis. Using ChIP-seq, we show that FBXL10 recruits RING1B to key specific genomic loci surrounding the key cell cycle and the adipogenic genes Cdk1, Uhrf1, Pparg1, and Pparg2 to repress adipogenesis. These results suggest that FBXL10 represses adipogenesis by targeting a noncanonical PRC1 complex to repress key genes (e.g. Pparg) that control conversion of pluripotent cells into the adipogenic lineage. 相似文献
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Hikaru Tsukazaki Shigenori Yaguchi Shusei Sato Hideki Hirakawa Yuichi Katayose Hiroyuki Kanamori Kanako Kurita Takeshi Itoh Masahiko Kumagai Satoshi Mizuno Masao Hamada Hiroyuki Fukuoka Ken-ichiro Yamashita John A. McCallum Masayoshi Shigyo Tadayuki Wako 《Molecular breeding : new strategies in plant improvement》2015,35(1):1-11
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Kanako Sugahara Yuko Kaneko Satoshi Ito Keisuke Yamanaka Hitoshi Sakio Kazuhiko Hoshizaki Wajiro Suzuki Norikazu Yamanaka Hiroaki Setoguchi 《Journal of plant research》2011,124(1):75-83
Japanese horse chestnut (Aesculus turbinata: Hippocastanaceae) is one of the typical woody plants that grow in temperate riparian forests in the Japanese Archipelago.
To analyze the phylogeography of this plant in the Japanese Archipelago, we determined cpDNA haplotypes for 337 samples from
55 populations covering the entire distribution range. Based on 1,313 bp of two spacers, we determined ten haplotypes that
are distinguished from adjacent haplotypes by one or two steps. Most of the populations had a single haplotype, suggesting
low diversity. Spatial analysis of molecular variance suggested three obvious phylogeographic structures in western Japan,
where Japanese horse chestnut is scattered and isolated in mountainous areas. Conversely, no clear phylogeographic structure
was observed from the northern to the southern limit of this species, including eastern Japan, where this plant is more common.
Rare and private haplotypes were also found in southwestern Japan, where Japanese horse chestnuts are distributed sparsely.
These findings imply that western Japan might have maintained a relatively large habitat for A. turbinata during the Quaternary climatic oscillations, while northerly regions could not. 相似文献
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Ito T Inoue M Akamatsu K Kusaka E Tanabe K Nishimoto S 《Bioorganic & medicinal chemistry letters》2011,21(12):3515-3518
The application of lanthanide complexes in the time-resolved fluorescence imaging of living cells has emerged in the last few decades, providing high-contrast images of cells through detection of the delayed emission. In the present study, we synthesized novel trivalent lanthanide complexes containing the cyclic peptide c(RGDfK) to visualize the αvβ3-integrin-expressing tumor cells. Conjugation of c(RGDfK) with the macrocyclic bipyridine ligand had little effect on the fluorescence properties of the complex, indicating that the coordinated lanthanide ion was well isolated from the peptide. Bright luminescence images of αvβ3-integrin-expressing U87-MG cells were successfully obtained by employing the probes. 相似文献
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Nakamura S Abe F Kawahigashi H Nakazono K Tagiri A Matsumoto T Utsugi S Ogawa T Handa H Ishida H Mori M Kawaura K Ogihara Y Miura H 《The Plant cell》2011,23(9):3215-3229
Seed dormancy is an adaptive mechanism and an important agronomic trait. Temperature during seed development strongly affects seed dormancy in wheat (Triticum aestivum) with lower temperatures producing higher levels of seed dormancy. To identify genes important for seed dormancy, we used a wheat microarray to analyze gene expression in embryos from mature seeds grown at lower and higher temperatures. We found that a wheat homolog of MOTHER OF FT AND TFL1 (MFT) was upregulated after physiological maturity in dormant seeds grown at the lower temperature. In situ hybridization analysis indicated that MFT was exclusively expressed in the scutellum and coleorhiza. Mapping analysis showed that MFT on chromosome 3A (MFT-3A) colocalized with the seed dormancy quantitative trait locus (QTL) QPhs.ocs-3A.1. MFT-3A expression levels in a dormant cultivar used for the detection of the QTL were higher after physiological maturity; this increased expression correlated with a single nucleotide polymorphism in the promoter region. In a complementation analysis, high levels of MFT expression were correlated with a low germination index in T1 seeds. Furthermore, precocious germination of isolated immature embryos was suppressed by transient introduction of MFT driven by the maize (Zea mays) ubiquitin promoter. Taken together, these results suggest that MFT plays an important role in the regulation of germination in wheat. 相似文献