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排序方式: 共有403条查询结果,搜索用时 15 毫秒
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Faizal I Dozen K Hong CS Kuroda A Takiguchi N Ohtake H Takeda K Tsunekawa H Kato J 《Journal of industrial microbiology & biotechnology》2005,32(11-12):542-547
Pseudomonas putida T-57 was isolated from an activated sludge sample after enrichment on mineral salts basal medium with toluene as a sole source of carbon. P. putida T-57 utilizes n-butanol, toluene, styrene, m-xylene, ethylbenzene, n-hexane, and propylbenzene as growth substrates. The strain was able to grow on toluene when liquid toluene was added to mineral salts basal medium at 10-90% (v/v), and was tolerant to organic solvents whose log P(ow) (1-octanol/water partition coefficient) was higher than 2.5. Enzymatic and genetic analysis revealed that P. putida T-57 used the toluene dioxygenase pathway to catabolize toluene. A cis-toluene dihydrodiol dehydrogenase gene (todD) mutant of T-57 was constructed using a gene replacement technique. The todD mutant accumulated o-cresol (maximum 1.7 g/L in the aqueous phase) when cultivated in minimal salts basal medium supplemented with 3% (v/v) toluene and 7% (v/v) 1-octanol. Thus, T-57 is thought to be a good candidate host strain for bioconversion of hydrophobic substrates in two-phase (organic-aqueous) systems. 相似文献
36.
Hidaka A Inoue K Kutsukake S Adachi M Kakuta Y Kojo S 《Bioorganic & medicinal chemistry letters》2005,15(11):2781-2785
A radical reaction of low-density lipoprotein (LDL) causes fragmentation and cross-link of apolipoprotein B-100 (apoB). LDL (50 microg/ml) was subjected to the well-studied oxidation with Cu(2+) (1.67 microM). The concentration of alpha-tocopherol decreased to 10% of the initial level during the first 30 min. After this lag time, the conjugated diene content, as measured by absorption at 234 nm, started increasing and the residual apoB at 512 kDa determined by immunoblot after SDS-PAGE (sodium dodecylsulfate-polyacrylamide gel electrophoresis) was also decreased. The particle size of LDL determined by nondenaturing gradient gel electrophoresis decreased steadily during the initial 120 min, when residual native apoB was only 30% of the initial level. Plasma was also oxidized with Cu(2+) (400 microM). Under this condition, a clear lag time was not observed and alpha-tocopherol content, apoB, and the LDL particle size were decreased simultaneously. Based on these experiments, we propose that an oxidation reaction is involved in the formation of small dense LDL. 相似文献
37.
Amemiya K Nakatani T Saito A Suzuki A Munakata H 《Biochimica et biophysica acta》2005,1724(1-2):94-99
The glycosaminoglycan hyaluronan (HA) is involved in a variety of functions such as cell migration, adhesion, activation of intracellular signaling, metastasis, inflammation and wound repair. These functions of HA are mediated via HA-binding proteins (HABPs). To derive details of the HA-binding site in HABPs, here, we panned a random peptide display library expressed on the E. coli flagellin protein using HA-coated plates. Using this random peptide display library, 40 positive clones were obtained and the nucleotide sequences were determined. As a result, an Arg-Arg sequence, in addition to the known B-X7-B motif, was found to bind to HA. A binding experiment using the IAsys resonant mirror biosensor verified that a peptide containing an Arg-Arg sequence binds to HA. 相似文献
38.
Fujita N Miyamoto T Imai J Hosogane N Suzuki T Yagi M Morita K Ninomiya K Miyamoto K Takaishi H Matsumoto M Morioka H Yabe H Chiba K Watanabe S Toyama Y Suda T 《Biochemical and biophysical research communications》2005,338(4):1890-1896
Intervertebral disc (IVD) consists of a soft gelatinous material in its center, the nucleus pulposus (NP), bounded peripherally by fibrocartilage, annulus fibrosus (AF). Despite the number of patients with IVD degeneration, gene expression analysis has not been undertaken in NP and therefore little is known about the molecular markers expressed in NP. Here, we undertook a microarray screen in NP with the other nine tissues to identify the specific cell surface markers for NP. Five membrane associating molecules out of 10,490 genes were identified as highly expressing genes in NP compared with the other tissues. Among them, we identified CD24, a glycosylphosphatidylinositol (GPI) anchor protein as a cell surface marker for NP. CD24 expression was also detected in the herniated NP and chordoma, a malignant primary tumor derived from notochordal cells, while it was absent in chondrosarcoma. Therefore, CD24 is a molecular marker for NP as well as the diseases of IVD. 相似文献
39.
Shigematsu T Era S Mizuno Y Ninomiya K Kamegawa Y Morimura S Kida K 《Applied microbiology and biotechnology》2006,72(2):401-415
Abstract
We constructed a mesophilic anaerobic chemostat that was continuously
fed with synthetic wastewater containing propionate as the sole source
of carbon and energy. Steady-state conditions were achieved below the
critical dilution rate of 0.3 d
−1
with almost complete substrate degradation. The propionate-degrading
methanogenic communities in the chemostat at dilution rates of 0.01,
0.08, and 0.3 d
−1
were analyzed using molecular biological techniques. Fluorescence in
situ hybridization with archaeal and bacterial domain-specific probes
showed that archaeal cells predominated throughout the three dilution
rates. Archaeal-16S rRNA gene clone library analysis and quantitative
real-time polymerase chain reaction studies showed that hydrogenotrophic
methanogen rRNA genes closely related to
Methanoculleus
was detected at a dilution rate of 0.01 d
−1
, whereas rRNA genes closely related to the
Methanoculleus
and
Methanospirillum
genera were detected at dilution rates of 0.08 and 0.3 d
−1
. The aceticlastic methanogen,
Methanosaeta
, was detected throughout the three dilution rates. Bacterial-rRNA gene
clone library analysis and denaturing gradient gel electrophoresis
demonstrated that rRNA genes affiliated with the genus
Syntrophobacter
predominated at the low dilution rate, whereas rRNA genes affiliated
with the phylum
Firmicutes
predominated at the higher dilution rates. A significant number of rRNA
genes affiliated with the genus
Pelotomaculum
were detected at dilution rate of 0.3 d
−1
. The diversity of genes encoding acetate kinase agreed closely with the
results of the rRNA gene analysis. The dilution rates significantly
altered the archaeal and bacterial communities in the propionate-fed
chemostat. 相似文献
40.