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961.
区域的物种多样性监测和评估是制定保护和管理策略的重要基础。为评估小相岭山系大中型兽类多样性现状,于2018年1月至2020年11月,以区域内最具代表性的四川栗子坪国家级自然保护区和四川冶勒省级自然保护区为主要研究区域,采用红外相机公里网格陷阱法进行了调查 (n = 86台)。累计完成19 982个相机工作日,获得大中型兽类独立有效照片3 304张,共记录大中型野生兽类18种,隶属于4目12科。其中,国家一级重点保护野生动物有大熊猫 (Ailuropoda melanoleuca)、林麝 (Moschus berezovskii) 和金猫 (Catopuma temminckii);国家二级重点保护野生动物有亚洲黑熊 (Ursus thibetanus)、中华小熊猫 (Ailurus styani)、水鹿 (Rusa unicolor) 等10种,且水鹿为该区域的新分布记录。相对多度指数排名前五的种类分别是毛冠鹿 (Elaphodus cephalophus)、中华鬣羚 (Capricornis milneedwardsii)、野猪 (Sus scrofa)、黄喉貂 (Martes flavigula) 和猪獾 (Arctonyx collaris)。由于面积大小、海拔范围的不同,两个保护区在大中型兽类组成和相对多度方面存在一定的差异。放牧是小相岭山系两个自然保护区最主要的人为干扰。本调查为小相岭山系保护区大中型兽类及人为活动状况提供了基础数据,有助于保护区后续的保护和管理。 相似文献
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Kim Seong Soon Hwang Kyu-Seok Kan Hyemin Yang Jung Yoon Son Yuji Shin Dae-Seop Lee Byung Hoi Chae Chong Hak Bae Myung Ae 《Neurochemical research》2022,47(8):2294-2306
Neurochemical Research - Paraquat is a polar herbicide protecting plant products against invasive species, it requires careful manipulation and restricted usage because of its harmful potentials.... 相似文献
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Kai Jiang Ce Zhang Durgarao Guttula Fan Liu Jeroen A. van?Kan Christophe Lavelle Krzysztof Kubiak Antoine Malabirade Alain Lapp Véronique Arluison Johan R.C. van?der?Maarel 《Nucleic acids research》2015,43(8):4332-4341
Hfq is a bacterial pleiotropic regulator that mediates several aspects of nucleic acids metabolism. The protein notably influences translation and turnover of cellular RNAs. Although most previous contributions concentrated on Hfq''s interaction with RNA, its association to DNA has also been observed in vitro and in vivo. Here, we focus on DNA-compacting properties of Hfq. Various experimental technologies, including fluorescence microscopy imaging of single DNA molecules confined inside nanofluidic channels, atomic force microscopy and small angle neutron scattering have been used to follow the assembly of Hfq on DNA. Our results show that Hfq forms a nucleoprotein complex, changes the mechanical properties of the double helix and compacts DNA into a condensed form. We propose a compaction mechanism based on protein-mediated bridging of DNA segments. The propensity for bridging is presumably related to multi-arm functionality of the Hfq hexamer, resulting from binding of the C-terminal domains to the duplex. Results are discussed in regard to previous results obtained for H-NS, with important implications for protein binding related gene regulation. 相似文献
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Typhoid fever remains a public health threat in many countries. A positive result in traditional culture is a gold-standard for typhoid diagnosis, but this method is time consuming and not sensitive enough for detection of samples containing a low copy number of the target organism. The availability of the loop-mediated isothermal amplification (LAMP) assay, which offers high speed and simplicity in detection of specific targets, has vastly improved the diagnosis of numerous infectious diseases. However, little research efforts have been made on utilizing this approach for diagnosis of Salmonella enterica serovar Typhi by targeting a single and specific gene. In this study, a LAMP assay for rapid detection of S. Typhi based on a novel marker gene, termed STY2879-LAMP, was established and evaluated with real-time PCR (RT-PCR). The specificity tests showed that STY2879 could be amplified in all S. Typhi strains isolated in different years and regions in China, whereas no amplification was observable in non-typhoidal strains covering 34 Salmonella serotypes and other pathogens causing febrile illness. The detection limit of STY2879-LAMP for S. Typhi was 15 copies/reaction in reference plasmids, 200 CFU/g with simple heat-treatment of DNA extracted from simulated stool samples and 20 CFU/ml with DNA extracted from simulated blood samples, which was 10 fold more sensitive than the parallel RT-PCR control experiment. Furthermore, the sensitivity of STY2879-LAMP and RT-PCR combining the traditional culture enrichment method for simulated stool and blood spiked with lower S. Typhi count during the 10 h enrichment time was also determined. In comparison with LAMP, the positive reaction time for RT-PCR required additional 2-3 h enrichment time for either simulated stool or blood specimens. Therefore, STY2879-LAMP is of practical value in the clinical settings and has a good potential for application in developing regions due to its easy-to-use protocol. 相似文献
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Kun Xu Shanshan Li Wen Yang Kan Li Yuwei Bai Yueyang Xu Jin Jin Yingying Wang Mark Bartlam 《PloS one》2015,10(4)
Biofilms are important for cell communication and growth in most bacteria, and are responsible for a number of human clinical infections and diseases. TpbA (PA3885) is a dual specific tyrosine phosphatase (DUSP) that negatively regulates biofilm formation in the opportunistic pathogen Pseudomonas aeruginosa PAO1 by converting extracellular quorum sensing signals into internal gene cascade reactions that result in reduced biofilm formation. We have determined the three-dimensional crystal structure of wild-type TpbA from P. aeruginosa PAO1 in the phosphate-bound state and a TpbA (C132S) mutant with phosphotyrosine. Comparison between the phosphate-bound structure and the previously reported ligand-free TpbA structure reveals the extent of conformational changes that occur upon substrate binding. The largest changes occur in the functional loops that define the substrate binding site, including the PTP, general acid and α4-α5 loops. We further show that TpbA efficiently catalyzes the hydrolysis of two phosphotyrosine peptides derived from the periplasmic domain of TpbB (YfiN, PA1120), with a strong preference for dephosphorylating Tyr48 over Tyr62. This work adds to the small repertoire of DUSP structures in both the ligand-free and ligand-bound states, and provides a starting point for further study of the role of TpbA in biofilm formation. 相似文献
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