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571.
Role of the fixGHI region of Azorhizobium caulinodans in free-living and symbiotic nitrogen fixation 总被引:4,自引:0,他引:4
Karine Mandon P.Alexandre Kaminski Christophe Mougel Nicole Desnoues Bernard Dreyfus Claudine Elmerich 《FEMS microbiology letters》1993,114(2):185-189
Abstract Rat intestinal epithelial cells were isolated and the activity of the calcium- and phospholipid-dependent protein kinase C (PKC) was investigated. The stimulation of activity by Escherichia coli heat stable enterotoxin (STa) was about 5-fold compared to control activity (16.91 ± 1.69 vs 93.56 ± 10.40 nmol/mg protein/min) and was dose dependent. Maximum enzyme activity was observed after incubation for 1 min with 6 ng of purified STa. The synergistic effects of calcium, phosphatidylserine and diolein on the enzyme activity were noted both in control and STa-treated cells. Staurosporine, a potent PKC inhibitor, significantly reduced the enzyme activity. Autoradiographic analysis of polyacrylamide gel electrophoresis revealed that pretreatment of the cells with STa also resulted in the phosphorylation of specific membrane proteins each with a molecular mass of 37 kDa, 100 kDa and 140 kDa. However, STa had no direct role on the enzyme activity. Our results, therefore, provide evidence for the involvement of PKC in STa-induced signal transduction in rat enterocytes. 相似文献
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Ida S. Opstad Florian Strhl Marcus Fantham Colin Hockings Oliver Vanderpoorten Francesca W. van Tartwijk Julie Qiaojin Lin Jean‐Claude Tinguely Firehun T. Dullo Gabriele S. Kaminski‐Schierle Balpreet S. Ahluwalia Clemens F. Kaminski 《Journal of biophotonics》2020,13(6)
Large fields of view (FOVs) in total internal reflection fluorescence microscopy (TIRFM) via waveguides have been shown to be highly beneficial for single molecule localisation microscopy on fixed cells [1,2] and have also been demonstrated for short‐term live‐imaging of robust cell types [3‐5], but not yet for delicate primary neurons nor over extended periods of time. Here, we present a waveguide‐based TIRFM set‐up for live‐cell imaging of demanding samples. Using the developed microscope, referred to as the ChipScope, we demonstrate successful culturing and imaging of fibroblasts, primary rat hippocampal neurons and axons of Xenopus retinal ganglion cells (RGCs). The high contrast and gentle illumination mode provided by TIRFM coupled with the exceptionally large excitation areas and superior illumination homogeneity offered by photonic waveguides have potential for a wide application span in neuroscience applications. 相似文献
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B K Kudelin Iu L Kaminski? I F Ivanova S S Gavrilin 《Biokhimii?a (Moscow, Russia)》1979,44(2):368-371
New solvent systems--dioxane--isopropanol--water--ammonium (4:2:4:1) and dioxane--water--ammonium (6:4:1)--for rapid qualitative and quantitative estimation of nucleosides, nucleoside-5'-mono-, di- and triphosphates by thin layer chromatography is Silufol UV-254 are proposed. The Rf values for 40 ribo- and deoxyribonucleotides of these systems are given. 相似文献
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Emilie Jaune Elisa Cavazza Cyril Ronco Oleksandr Grytsai Patricia Abbe Nedra Tekaya Marwa Zerhouni Guillaume Beranger Lisa Kaminski Frdric Bost Maeva Gesson Meri Tulic Paul Hofman Robert Ballotti Thierry Passeron Thomas Botton Rachid Benhida Stphane Rocchi 《Cell death & disease》2021,12(1)
In the search of biguanide-derived molecules against melanoma, we have discovered and developed a series of bioactive products and identified the promising new compound CRO15. This molecule exerted anti-melanoma effects on cells lines and cells isolated from patients including the ones derived from tumors resistant to BRAF inhibitors. Moreover, CRO15 was able to decrease viability of cells lines from a broad range of cancer types. This compound acts by two distinct mechanisms. First by activating the AMPK pathway induced by a mitochondrial disorder. Second by inhibition of MELK kinase activity, which induces cell cycle arrest and activation of DNA damage repair pathways by p53 and REDD1 activation. All of these mechanisms activate autophagic and apoptotic processes resulting in melanoma cell death. The strong efficacy of CRO15 to reduce the growth of melanoma xenograft sensitive or resistant to BRAF inhibitors opens interesting perspective.Subject terms: Melanoma, Cell death 相似文献