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141.
Previously it was shown that stimulation of the P2Y12 receptor activates PKB signalling in C6 glioma cells [K. Van Kolen and H. Slegers, J. Neurochem. 89, 442.]. In the present study, the mechanisms involved in this response were further elucidated. In cells transfected with the Gbetagamma-scavenger beta-ARK1/GRK2 or Rap1GAPII, stimulation with 2MeSADP failed to enhance PKB phosphorylation demonstrating that the signalling proceeds through Gbetagamma-subunits and Rap1. Moreover, Rap1-GTP pull-down assays revealed that P2Y12 receptor stimulation induced a rapid activation of Rap1. Treatment of cells with the Ca2+ chelator BAPTA-AM and inhibition of Src and PLD2 with PP2 or 1-butanol, respectively, abrogated P2Y12 receptor-mediated activation of Rap1 and PKB. In addition inhibition of PKCzeta decreased basal and 2MeSADP-stimulated phosphorylation of PKB indicating a role for this PKC isoform in PKB signalling. Although the increased PKB phosphorylation was abolished in the presence of the IGF-I receptor tyrosine kinase inhibitor AG 1024, 2MeSADP did not significantly increase receptor phosphorylation. Nevertheless, phosphorylation of a 120 kDa IGF-I receptor-associated protein was observed. The latter protein was identified by MALDI-TOF/TOF-MS as the proline-rich tyrosine kinase 2 (Pyk2) that co-operates with Src in a PLD2-dependent manner. Consistent with the signalling towards Rap1 and PKB, activation of Pyk2 was abrogated by Ca2+ chelation, inhibition of PLD2 and IGF-I receptor tyrosine kinase activity. In conclusion, the data reveal a novel type of cross-talk between P2Y12 and IGF-I receptors that proceeds through Gbetagamma-, Ca2+-and PLD2-dependent activation of the Pyk2/Src pathway resulting in GTP-loading of Rap1 required for an increased PKB phosphorylation. 相似文献
142.
Solubilization of organic and inorganic phosphates by three highly efficient soil bacterial isolates
Mohammad Ali Malboobi Parviz Owlia Mandana Behbahani Elaheh Sarokhani Sara Moradi Bagher Yakhchali Ali Deljou Kambiz Morabbi Heravi 《World journal of microbiology & biotechnology》2009,25(8):1471-1477
Screening soil samples collected from a diverse range of slightly alkaline soil types, we have isolated 22 competent phosphate
solubilizing bacteria (PSB). Three isolates identified as Pantoea agglomerans strain P5, Microbacterium laevaniformans strain P7 and Pseudomonas putida strain P13 hydrolyzed inorganic and organic phosphate compounds effectively. Bacterial growth rates and phosphate solubilization
activities were measured quantitatively under various environmental conditions. In general, a close association was evident
between phosphate solubilizing ability and growth rate which is an indicator of active metabolism. All three PSB were able
to withstand temperature as high as 42°C, high concentration of NaCl upto 5% and a wide range of initial pH from 5 to 11 while
hydrolyzing phosphate compounds actively. Such criteria make these isolates superior candidates for biofertilizers that are
capable of utilizing both organic and mineral phosphate substrates to release absorbable phosphate ion for plants. 相似文献
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145.
Joachim Ahlert Jürgen Distler Kambiz Mansouri W. Piepersberg 《Archives of microbiology》1997,168(2):102-113
Eight new genes, strO-stsABCDEFG, were identified by sequencing DNA in the gene cluster that encodes proteins for streptomycin production of Streptomyces griseus N2-3-11. The StsA (calculated molecular mass 43.5 kDa) and StsC (45.5 kDa) proteins – together with another gene product,
StrS (39.8 kDa), encoded in another operon of the same gene cluster – show significant sequence identity and are members of
a new class of pyridoxal-phosphate-dependent aminotransferases that have been observed mainly in the biosynthetic pathways
for secondary metabolites. The aminotransferase activity was demonstrated for the first time by identification of the overproduced
and purified StsC protein as the l-glutamine:scyllo-inosose aminotransferase, which catalyzes the first amino transfer in the biosynthesis of the streptidine subunit of streptomycin.
The stsC and stsA genes each hybridized specifically to distinct fragments in the genomic DNA of most actinomycetes tested that produce diaminocyclitolaminoglycosides.
In contrast, only stsC, but not stsA, hybridized to the DNA of Streptomyces hygroscopicus ssp. glebosus, which produces the monoaminocyclitol antibiotic bluensomycin; this suggests that both genes are specifically used in the
first and second steps of the cyclitol transamination reactions. Sequence comparison studies performed with the deduced polypeptides
of the genes adjacent to stsC suggest that the enzymes encoded by some of these genes [strO (putative phosphatase gene), stsB (putative oxidoreductase gene), and stsE (putative phosphotransferase gene)] also could be involved in (di-)aminocyclitol synthesis.
Received: 7 January 1997 / Accepted: 24 March 1997 相似文献
146.
Hosseini Elnaz Rajaei Ahmad Tabatabaei Meisam Mohsenifar Afshin Jahanbin Kambiz 《Food biophysics》2020,15(2):216-228
Food Biophysics - The aim of this study was to obtain a stable flaxseed oil (FSO)-in-water Pickering emulsion (PE) stabilized by chitosan (CS)-myristic acid (MA) nanogels and to investigate the... 相似文献
147.
Ali Amirahmadi Zahra Emkanjoo Reza Mollazadeh Kambiz Mozaffari 《Indian pacing and electrophysiology journal》2013,13(1):43-44
A 27 year-old- lady was evaluated due to recurrent ventricular tachycardia. After performing echocardiography and cardiac MRI, she was found to have large pericardial cyst. Pathologic examination confirmed it as mesothelial pericardial cyst. Up to our knowledge it is the first presentation of simple pericardial cyst as ventricular a tachycardia. 相似文献
148.
Mahrad Nassirkhani Reza Vafai Shoushtari Mahtab Rahmat Abadi 《Zoology in the Middle East.》2016,62(1):78-84
The pseudoscorpion Hysterochelifer afghanicus Beier, 1966 is redescribed and illustrated based on 21 specimens (13♂, 8♀) collected from different sites in northern, western, and southern Iran. A number of morphological characters and new provincial records are given. In addition, a key for 13 species of the pseudoscorpion genus Hysterochelifer Chamberlin, 1932 is proposed. 相似文献
149.
Analysis of three late varicella-zoster virus proteins, a 125,000-molecular-weight protein and gp1 and gp3 总被引:8,自引:4,他引:4 下载免费PDF全文
Two monoclonal antibodies were prepared against varicella-zoster virus proteins. One of the monoclonal antibodies (10.2) reacted only with the nuclei of infected cells and immunoprecipitated one nonglycosylated late viral protein (125,000 molecular weight). The other monoclonal antibody (19.1) with neutralizing activity, reacted with membrane antigens of infected cells and with the varicella-zoster virus envelope and immunoprecipitated two late major viral glycoproteins (gp1 and gp3). Synthesis of the 125,000-molecular-weight protein, gp1, and gp3 began at 20 to 22 h postinfection, 2 h after the peak of viral DNA synthesis, and continued until 29 h postinfection, when the first progeny virus appeared in infected cells. Pulse-chase experiments showed that during pulse-labeling, only gp1 was detected, whereas during the chase period, gp1 as well as gp3 was detected in infected cells. Under nonreducing conditions, gp3 migrated in sodium dodecyl sulfate-polyacrylamide gel electrophoresis as a 130,000-molecular-weight protein as compared with the 62,000-molecular-weight species obtained when gels were resolved under reducing conditions. This finding indicates that gp3 is a dimer that is disulfide linked. 相似文献