全文获取类型
收费全文 | 419篇 |
免费 | 35篇 |
专业分类
454篇 |
出版年
2023年 | 3篇 |
2019年 | 4篇 |
2018年 | 8篇 |
2017年 | 3篇 |
2016年 | 7篇 |
2015年 | 10篇 |
2014年 | 14篇 |
2013年 | 18篇 |
2012年 | 27篇 |
2011年 | 25篇 |
2010年 | 18篇 |
2009年 | 15篇 |
2008年 | 17篇 |
2007年 | 22篇 |
2006年 | 21篇 |
2005年 | 11篇 |
2004年 | 20篇 |
2003年 | 15篇 |
2002年 | 13篇 |
2001年 | 13篇 |
2000年 | 12篇 |
1999年 | 11篇 |
1997年 | 4篇 |
1996年 | 3篇 |
1995年 | 4篇 |
1994年 | 4篇 |
1992年 | 7篇 |
1991年 | 8篇 |
1990年 | 13篇 |
1989年 | 5篇 |
1988年 | 4篇 |
1987年 | 7篇 |
1986年 | 8篇 |
1984年 | 5篇 |
1983年 | 4篇 |
1982年 | 3篇 |
1981年 | 2篇 |
1980年 | 2篇 |
1979年 | 5篇 |
1978年 | 2篇 |
1977年 | 3篇 |
1976年 | 6篇 |
1975年 | 4篇 |
1974年 | 10篇 |
1972年 | 6篇 |
1971年 | 4篇 |
1968年 | 2篇 |
1967年 | 2篇 |
1966年 | 6篇 |
1960年 | 2篇 |
排序方式: 共有454条查询结果,搜索用时 0 毫秒
91.
Ghantasala S Sameer Kumar Abhilash K Venugopal Anita Mahadevan Santosh Renuse H C Harsha Nandini A Sahasrabuddhe Harsh Pawar Rakesh Sharma Praveen Kumar Sudha Rajagopalan Keith Waddell Yarappa L Ramachandra Parthasarathy Satishchandra Raghothama Chaerkady T S Keshava Prasad K Shankar Akhilesh Pandey 《Clinical proteomics》2012,9(1):12
92.
18-Hydroxyprogesterone is conveniently prepared from 3beta-acetoxypregn-5-en-20beta-ol by a modified route. 3beta-Acetoxy-18-iodopregn-5-en-20-one, obtained by the hypoiodite-photolysis procedure and oxidation, is treated with methanolic silver acetate to give the 18, 20-epoxy-20-methoxy derivative, which crystallises directly without need for chromatography. Hydrolysis of the 3-acetate, and a modified Oppenauer oxidation, gave 18-hydroxy-progesterone in 24% over-all yield. 相似文献
93.
Preferential binding of Escherichia coli RecF protein to gapped DNA in the presence of adenosine (gamma-thio) triphosphate. 总被引:1,自引:0,他引:1 下载免费PDF全文
Escherichia coli RecF protein binds, but does not hydrolyze, ATP. To determine the role that ATP binding to RecF plays in RecF protein-mediated DNA binding, we have determined the interaction between RecF protein and single-stranded (ss)DNA, double-stranded (ds)DNA, and dsDNA containing ssDNA regions (gapped [g]DNA) either alone or in various combinations both in the presence and in the absence of adenosine (gamma-thio) triphosphate, gamma-S-ATP, a nonhydrolyzable ATP analog. Protein-DNA complexes were analyzed by electrophoresis on agarose gels and visualized by autoradiography. The type of protein-DNA complexes formed in the presence of gamma-S-ATP was different with each of the DNA substrates and from those formed in the absence of gamma-S-ATP. Competition experiments with various combinations of DNA substrates indicated that RecF protein preferentially bound gDNA in the presence of gamma-S-ATP, and the order of preference of binding was gDNA > dsDNA > ssDNA. Since gDNA has both ds- and ssDNA components, we suggest that the role for ATP in RecF protein-DNA interactions in vivo is to confer specificity of binding to dsDNA-ssDNA junctions, which is necessary for catalyzing DNA repair and recombination. 相似文献
94.
B. S. Kotlia M. S. Bhalla C. Sharma G. Rajagopalan R. Ramesh M. S. Chauhan P. D. Mathur S. Bhandari S. T. Chacko 《Palaeogeography, Palaeoclimatology, Palaeoecology》1997,130(1-4):307-322
A 52 m thick upper Pleistocene and Holocene terrestrial succession in the Bhimtal-Naukuchiatal basin, south-central Kumaun Himalaya, India was studied using chronological, palaeontological, palynological and δ13C measurements. The section recorded evidence for climatic changes. At least two phases of arid climate and one phase of humid climate were recognised. Preliminary palaeomagnetic studies revealed a reversal of polarity, presumably correlatable with the Mono Lake excursion. Prior to this, no reversal event in the upper Pleistocene-Holocene terrestrial sediments of Indian subcontinent is known. A fossiliferous horizon, discovered in the lower part of the section, consisted of Sorex and Mus. This is the only report of a Late Pleistocene micromammalian assemblage in the Kumaun Himalaya. 相似文献
95.
R F Weaver K V Rajagopalan P Handler W L Byrne 《The Journal of biological chemistry》1971,246(7):2015-2020
96.
E Cayanis R Rajagopalan W L Cleveland I S Edelman B F Erlanger 《The Journal of biological chemistry》1986,261(11):5094-5103
A monoclonal antibody (8G11-C6) that is directed to a region near the ligand-binding site of the glucocorticoid receptor was obtained by an auto-anti-idiotypic route, using a derivative of triamcinolone coupled to thyroglobulin to immunize a mouse. The resulting hybridomas were screened for anti-idiotypic antibody (anti-antisteroid) with Fab fragments of affinity-purified polyclonal rabbit anti-triamcinolone antibody. The anti-idiotypes were then screened for binding to rat cytosol glucocorticoid receptor by a depletion procedure, yielding a clone, 8G11-C6, whose specificity for receptor was verified by sucrose density and Western blot analyses. Depletion was not significantly reduced by prelabeling the cytosol with [3H]triamcinolone acetonide. The anti-idiotype (8G11-C6) bound to Fab fragments of antisteroid and to partially purified receptor in a concentration-dependent manner. Both binding reactions were inhibited only by rabbit serum albumin conjugates of steroids known to bind to the glucocorticoid receptors. Triamcinolone derivatives of lysine and of oligopeptides containing up to six amino acids inhibited the binding of the anti-idiotype to the Fab fragments but not to the receptor, implying that the target epitope of the antisteroid antibody may be closer to its glucocorticoid-binding site than the cross-reacting epitope of the receptor. Our findings demonstrate further the versatility of the auto-anti-idiotypic route for the preparation of anti-receptor antibodies. 相似文献
97.
Pepsin from pepsinogen. Preparation and properties 总被引:17,自引:0,他引:17
98.
Ultracentrifugal characterization of pepsin and pepsinogen 总被引:1,自引:0,他引:1
99.
The carbon monoxide oxidases (COXs) purified from the carboxydotrophic bacteria Pseudomonas carboxydohydrogena and Pseudomonas carboxydoflava were found to be molybdenum hydroxylases, identical in cofactor composition and spectral properties to the recently characterized enzyme from Pseudomonas carboxydovorans (O. Meyer, J. Biol. Chem. 257:1333-1341, 1982). All three enzymes exhibited a cofactor composition of two flavin adenine dinucleotides, two molybdenums, eight irons and eight labile sulfides per dimeric molecule, typical for molybdenum-containing iron-sulfur flavoproteins. The millimolar extinction coefficient of the COXs at 450 nm was 72 (per two flavin adenine dinucleotides), a value similar to that of milk xanthine oxidase and chicken liver xanthine dehydrogenase at 450 nm. That molybdopterin, the novel prosthetic group of the molybdenum cofactor of a variety of molybdoenzymes (J. Johnson and K. V. Rajagopalan, Proc. Natl. Acad. Sci. U.S.A. 79:6856-6860, 1982) is also a constituent of COXs from carboxydotrophic bacteria is indicated by the formation of identical fluorescent cofactor derivatives, by complementation of the nitrate reductase activity in extracts of Neurospora crassa nit-l, and by the presence of organic phosphate additional to flavin adenine dinucleotides. Molybdopterin is tightly but noncovalently bound to the protein. COX, sulfite oxidase, xanthine oxidase, and xanthine dehydrogenase each contains 2 mol of molybdopterin per mol of enzyme. The presence of a trichloroacetic acid-releasable, so-far-unidentified, phosphorous-containing moiety in COX is suggested by the results of phosphate analysis. 相似文献
100.
The structure of a molybdopterin precursor. Characterization of a stable, oxidized derivative 总被引:4,自引:0,他引:4
J L Johnson M M Wuebbens K V Rajagopalan 《The Journal of biological chemistry》1989,264(23):13440-13447
An oxidized pterin species, termed compound Z, has been isolated from molybdenum cofactor-deficient mutants of Escherichia coli and shown to be the direct product of oxidation of a molybdopterin precursor which accumulates in these mutants. The complete structural characterization of compound Z has been accomplished. A carbonyl function at C-1' of the 6-alkyl side chain can be reacted with 2,4-dinitrophenylhydrazine to yield a phenylhydrazone and can be reduced with borohydride, producing a mixture of two enantiomers, each with a hydroxyl group on C-1'. Compound Z contains one phosphate/pterin and no sulfur. The phosphate group is insensitive to alkaline phosphatase and to a number of phosphodiesterases but is quantitatively released as inorganic phosphate by mild acid hydrolysis. From 31P and 1H NMR of compound Z it was inferred that the phosphate is bound to C-2' and C-4' of a 4-carbon side chain, forming a 6-membered cyclic structure. Mass spectral analysis showed an MH+ ion with an exact mass of 344.0401 corresponding to the molecular formula C10H11N5O7P, confirming the proposed structure. 相似文献