Cost effective surface functionalization of silver nanoparticles for high yield immobilization of Aspergillus oryzae β-galactosidase and its application in lactose hydrolysis

The present study demonstrates synthesis, characterization and surface functionalization of silver nanoparticles (AgNPs) via glutaraldehyde for high yield immobilization of Aspergillus oryzae β-galactosidase. Soluble β-galactosidase (SβG), enzyme adsorbed on unmodified AgNPs (UβG) and surface modified AgNPs (MβG) showed same pH-optima at pH 4.5. However, it was observed that MβG exhibited enhanced pH stability toward acidic and alkaline sides, and increased temperature resistance as compared to SβG and UβG. Michaelis constant, K_m was increased nearly three-folds for MβG while V_max for soluble and MβG was 0.515 mM/min and 0.495 mM/min, respectively. Furthermore, MβG showed greater resistance to product inhibition mediated by galactose as compared to it soluble counterpart and exhibited excellent catalytic activity even after its fourth successive reuse. The remarkable bioconversion rates of lactose from milk in batch reactors further revealed an attractive catalytic efficiency of β-galactosidase adsorbed on surface functionalized AgNPs thereby promoting its use in the production of lactose free dairy products.     

Changes in physiology and protein abundance in salt-stressed wheat chloroplasts

Leaves are the final site of salinity perception through the roots. To better understand how wheat chloroplasts proteins respond to salt stress, the study aimed to the physiochemical and comparative proteomics analysis. Seedlings (12-days-old) were exposed to 150 mM NaCl for 1, 2, or 3 days. Na(+) ions were rapid and excessively increase in roots, stems and leaves. Photosynthesis and transpiration rate, stomatal conductance, and relative water content decreased whereas the level of proline increased. Statistically significant positive correlations were found among the content of hydrogen peroxide, activity of catalase, and superoxide dismutase under salt stress in wheat. Protein abundance within the chloroplasts was examined by two-dimensional electrophoresis. More than 100 protein spots were reproducibly detected on each gel, 21 protein spots were differentially expressed during salt treatment. Using linear quadruple trap-Fourier transform ion cyclotron resonance (LTQ-FTICR) hybrid mass spectrometry, 65 unique proteins assigned in the differentially abundant spots. Most proteins were up-regulated at 2 and 3 days after being down-regulated at 1 day. Others showed only slight responses after 3 days of treatment, including Rubisco, glutamate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, isocitrate dehydrogenase, photosystem I, and pyridoxal biosynthesis protein PDX1.2 and PDX1.3. The ATP synthase (α, β, and γ) and V-type proton ATPase subunits were down-regulated resulting showed negative impact by Na(+) on the photosynthetic machinery. This ephemeral increase and subsequent decrease in protein contents may demonstrate a counterbalancing influence of identified proteins. Several proteins such as cytochrome b6-f (Cyt b6-f), germin-like-protein, the γ-subunit of ATP synthase, glutamine synthetase, fructose-bisphosphate aldolase, S-adenosylmethionine synthase, carbonic anhydrase were gradually up-regulated during the period of treatment, which can be identified as marker proteins.     

Salt stress induction of some key antioxidant enzymes and metabolites in eight Iranian wild almond species

The present work describes the changes in the activities of key antioxidant enzymes and the levels of some metabolites in relation to salt tolerance in eight wild almond species. All the species were exposed to four levels of NaCl (control, 40, 80 and 120 mM). Plant fresh biomass, α-, γ- and δ-tocopherol, total soluble proteins, malondialdehyde (MDA_eq), H₂O₂, total phenolics, and the activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) were analyzed in leaves of salt-stressed and non-stressed plants of the eight almond species. In all the species, salt stress significantly enhanced the activities of SOD and POD, levels of total phenolics and γ- and δ-tocopherols. High levels of salt stress significantly depressed the levels of total soluble proteins, MDA and CAT activity, while salt stress did not significantly affect leaf H₂O₂ contents. Regression analysis showed that the relationship between salt levels and total soluble proteins, CAT, γ-tocopherol, MDA_eq, SOD and POD were statistically significant. Principal component analysis discriminated the almond species based on their degree of tolerance/sensitivity to saline conditions: Prunus reuteri and P. glauca were ranked as salt tolerant, P. lycioides and P. scoparia as moderately tolerant, and P. communis, P. eleagnifolia, P. arabica and P. orientalis as salt sensitive. The results could be used for selecting salt tolerant genotypes to be used as rootstocks for almond cultivation.     

Mild pretreatment and enzymatic saccharification of cellulose with recycled ionic liquids towards one-batch process

The development of second-generation bioethanol involves minimizing the energy input throughout the processing steps. We report here that efficient ionic liquid pretreatments of cellulose can be achieved with short duration times (20min) at mild temperature (45°C) with [Emim](+)[MeO(H)PO(2)](-) and at room temperature (25°C) with [Emim](+)[CH(3)COO](-). In these conditions, yields of glucose were increased by a factor of 3. In addition, the recycling of these two imidazolium-based ILs can be performed in maintaining their efficiency to pretreat cellulose. The short time and mild temperature of cellulose solubilization allowed a one-batch processing of [Emim](+)[MeO(H)PO(2)](-) IL-pretreatment and saccharification. In the range from 0 to 100% IL in an aqueous enzymatic medium, the glucose yields were improved at IL proportions between 10 and 40%. The maximum yield at 10% IL is very promising to consider one batch process as efficient as two-step process.     

Cryptic diversity among Western Palearctic tree frogs: Postglacial range expansion, range limits, and secondary contacts of three European tree frog lineages (Hyla arborea group)

We characterize divergence times, intraspecific diversity and distributions for recently recognized lineages within the Hyla arborea species group, based on mitochondrial and nuclear sequences from 160 localities spanning its whole distribution. Lineages of H. arborea, H. orientalis, H. molleri have at least Pliocene age, supporting species level divergence. The genetically uniform Iberian H. molleri, although largely isolated by the Pyrenees, is parapatric to H. arborea, with evidence for successful hybridization in a small Aquitanian corridor (southwestern France), where the distribution also overlaps with H. meridionalis. The genetically uniform H. arborea, spread from Crete to Brittany, exhibits molecular signatures of a postglacial range expansion. It meets different mtDNA clades of H. orientalis in NE-Greece, along the Carpathians, and in Poland along the Vistula River (there including hybridization). The East-European H. orientalis is strongly structured genetically. Five geographic mitochondrial clades are recognized, with a molecular signature of postglacial range expansions for the clade that reached the most northern latitudes. Hybridization with H. savignyi is suggested in southwestern Turkey. Thus, cryptic diversity in these Pliocene Hyla lineages covers three extremes: a genetically poor, quasi-Iberian endemic (H. molleri), a more uniform species distributed from the Balkans to Western Europe (H. arborea), and a well-structured Asia Minor-Eastern European species (H. orientalis).     

The convergent validity between two objective methods for quantifying training load in young taekwondo athletes

Various studies used objective heart rate (HR)-based methods to assess training load (TL). The common methods were Banister's Training Impulse (TRIMP; weights the duration using a weighting factor) and Edwards' TL (a summated HR zone score). Both the methods use the direct physiological measure of HR as a fundamental part of the calculation. To eliminate the redundancy of using various methods to quantify the same construct (i.e., TL), we have to verify if these methods are strongly convergent and are interchangeable. Therefore, the aim of this study was to investigate the convergent validity between Banister's TRIMP and Edwards' TL used for the assessment of internal TL. The HRs were recorded and analyzed during 10 training weeks of the preseason period in 10 male Taekwondo (TKD) athletes. The TL was calculated using Banister's TRIMP and Edwards' TL. Pearson product moment correlation coefficient was used to evaluate the convergent validity between the 2 methods for assessing TL. Very large to nearly perfect relationships were found between individual Banister's TRIMP and Edwards' TL (r values from 0.80 to 0.99; p < 0.001). Pooled Banister's TRIMP and pooled Edwards' TL (pooled data n = 284) were nearly largely correlated (r = 0.89; p < 0.05; 95% confidence interval: 0.86-0.91). In conclusion, these findings suggest that these 2 objective methods, measuring a similar construct, are interchangeable.     

The effect of training at the same time of day and tapering period on the diurnal variation of short exercise performances

The purpose of this investigation was to assess the effects of training and tapering at the same time of the day on the diurnal variations of short exercise performances. Thirty-one physically active men underwent 12 weeks of lower-extremity resistance training and 2 weeks of tapering. These subjects were matched and randomly assigned to a morning training group (MTG, training times 0700-0800 hours, n = 10), an evening training group (ETG, training times 1700-1800 hours, n = 11), and a control group (CG, completed all tests but did not train, n = 10). Muscular strength and power testing was conducted before (T0) and after 12 weeks of training (T1) and after 2 weeks of tapering (T2) in the morning (0700-0800 hours) and in the evening (1700-1800 hours). All morning and evening tests were performed in separate sessions (minimum interval = 36 hours) in a randomized design. In T0, the oral temperature and performances during the Wingate, vertical jump (squat jump and countermovement jump), and maximal voluntary contraction tests were higher in the evening than in the morning for all the groups. In T1, these diurnal variations were blunted in the MTG and persisted in the ETG and CG. In T2, the 2 weeks of tapering resulted in further time of day-specific adaptations and increases in short-term maximal performances. However, there was no significant difference in the relative increase between the MTG and the ETG after both training and tapering. From a practical point of view, if the time of competition is known, training and tapering sessions before a major competition must be conducted at the same time of the day at which one's critical performance is programmed. Moreover, if the time of the competition is not known, a tapering phase after resistance training program could be performed at any time of the day with the same benefit.     

Synthesis and antimicrobial activity of novel benzo[f]coumarin compounds

The acetyl benzo[f]coumarin condensed with phenyl hydrazine to afford the corresponding phenyl hydrazone which cyclized into the pyrazolyl benzocoumarin under Vilsmeier reaction conditions. The pyrazolylaldehyde was used as starting material for synthesis of other heterocyclic compounds containing pyrazolylbenzocoumarin moiety. The ethyl benzo[f]coumarin carboxylate were subjected to react with other reagents to synthesize thiazolidinyl and oxadiazolyl derivatives attached to benzocoumarin system. Some of novel synthesized compounds showed highly antibacterial and antifungal activities.     

The effect of warm-ups incorporating different volumes of dynamic stretching on 10- and 20-m sprint performance in highly trained male athletes

Recently, athletes have transitioned from traditional static stretching during warm-ups to incorporating dynamic stretching routines. However, the optimal volume of dynamic drills is yet to be identified. The aim of this repeated-measures study was to examine varying volumes (1, 2, and 3 sets) of active dynamic stretching (ADS) in a warm-up on 10- and 20-m sprint performance. With a within-subject design, 16 highly trained male participants (age: 20.9 ± 1.3 years; height: 179.7 ± 5.7 cm; body mass: 72.7 ± 7.9 kg; % body fat: 10.9 ± 2.4) completed a 5-minute general running warm-up before performing 3 preintervention measures of 10- to 20-m sprint. The interventions included 1, 2, and 3 sets of active dynamic stretches of the lower-body musculature (gastrocnemius, gluteals, hamstrings, quadriceps, and hip flexors) performed approximately 14 times for each exercise while walking (ADS1, ADS2, and ADS3). The active dynamic warm-ups were randomly allocated before performing a sprint-specific warm-up. Five minutes separated the end of the warm-up and the 3 postintervention measures of 10- to 20-m sprints. There were no significant time, condition, and interaction effects over the 10-m sprint time. For the 0- to 20-m sprint time, a significant main effect for the pre-post measurement (F = 10.81; p < 0.002), the dynamic stretching condition (F = 6.23; p = 0.004) and an interaction effect (F = 41.19; p = 0.0001) were observed. A significant decrease in sprint time (improvement in sprint performance) post-ADS1 (2.56%, p = 0.001) and post-ADS2 (2.61%, p = 0.001) was observed. Conversely, the results indicated a significant increase in sprint time (sprint performance impairment) post-ADS3 condition (2.58%, p = 0.001). Data indicate that performing 1-2 sets of 20 m of active dynamic stretches in a warm-up can enhance 20-m sprint performance. The results delineated that 3 sets of ADS repetitions could induce acute fatigue and impair sprint performance within 5 minutes of the warm-up.