全文获取类型
收费全文 | 6645篇 |
免费 | 484篇 |
国内免费 | 1篇 |
专业分类
7130篇 |
出版年
2023年 | 24篇 |
2022年 | 74篇 |
2021年 | 136篇 |
2020年 | 83篇 |
2019年 | 149篇 |
2018年 | 186篇 |
2017年 | 151篇 |
2016年 | 234篇 |
2015年 | 296篇 |
2014年 | 393篇 |
2013年 | 476篇 |
2012年 | 520篇 |
2011年 | 476篇 |
2010年 | 320篇 |
2009年 | 268篇 |
2008年 | 401篇 |
2007年 | 359篇 |
2006年 | 306篇 |
2005年 | 303篇 |
2004年 | 307篇 |
2003年 | 273篇 |
2002年 | 245篇 |
2001年 | 107篇 |
2000年 | 83篇 |
1999年 | 74篇 |
1998年 | 56篇 |
1997年 | 45篇 |
1996年 | 61篇 |
1995年 | 35篇 |
1994年 | 34篇 |
1993年 | 36篇 |
1992年 | 57篇 |
1991年 | 48篇 |
1990年 | 45篇 |
1989年 | 35篇 |
1988年 | 39篇 |
1987年 | 29篇 |
1986年 | 28篇 |
1985年 | 31篇 |
1984年 | 19篇 |
1983年 | 31篇 |
1982年 | 27篇 |
1981年 | 24篇 |
1980年 | 23篇 |
1979年 | 18篇 |
1977年 | 18篇 |
1976年 | 15篇 |
1975年 | 19篇 |
1974年 | 17篇 |
1972年 | 15篇 |
排序方式: 共有7130条查询结果,搜索用时 0 毫秒
41.
An antibody against secretogranin I (chromogranin B) is packaged into secretory granules 总被引:16,自引:8,他引:8 下载免费PDF全文
P Rosa U Weiss R Pepperkok W Ansorge C Niehrs E H Stelzer W B Huttner 《The Journal of cell biology》1989,109(1):17-34
We have investigated the sorting and packaging of secretory proteins into secretory granules by an immunological approach. An mAb against secretogranin I (chromogranin B), a secretory protein costored with various peptide hormones and neuropeptides in secretory granules of many endocrine cells and neurons, was expressed by microinjection of its mRNA into the secretogranin I-producing cell line PC12. An mAb against the G protein of vesicular stomatitis virus--i.e., against an antigen not present in PC12 cells--was expressed as a control. The intracellular localization and the secretion of the antibodies was studied by double-labeling immunofluorescence using the conventional and the confocal microscope, as well as by pulse-chase experiments. The secretogranin I antibody, like the control antibody, was transported along the secretory pathway to the Golgi complex. However, in contrast to the control antibody, which was secreted via the constitutive pathway, the secretogranin I antibody formed an immunocomplex with secretogranin I, was packaged into secretory granules, and was released by regulated exocytosis. Our results show that a constitutive secretory protein, unaltered by genetic engineering, can be diverted to the regulated pathway of secretion by its protein-protein interaction with a regulated secretory protein. The data also provide the basis for immunologically studying the role of luminally exposed protein domains in the biogenesis and function of regulated secretory vesicles. 相似文献
42.
The sibling species Drosophila melanogaster and D. simulans were collected at Laguna Verde, Veracruz, Mexico. D. melanogaster was found in significantly greater frequency than was D. simulans. Ten isofemale lines of each species were tested for egg to adult viability, desiccation resistance, and vagility. D. melanogaster surpassed D. simulans in all three characteristics. The findings are discussed with reference to the climatic conditions at Laguna Verde and the expected effect of such an environment on the relative frequencies of these species. The dichotomous results in regard to desiccation resistance and vagility that were observed between recently collected D. melanogaster and the Oregon-R laboratory stock of that species are also discussed. 相似文献
43.
Rosa Sorrentino Carlo Iannicola Sandro Costanzi Giulio Ratti Carolyn Hurley Roberto Tosi Nobuyuki Tanigaki 《Immunogenetics》1990,32(1):8-12
TR81 is a specificity closely related to or identical with DR3. In Caucasoids two amino acids, Tyr at position 26 and Arg at position 74 of HLA class II DR chains, have been found to be associated with the presence of TR81. Recently, a variant of DRBI *03 identified in American Blacks has been shown to possess Arg at position 74 but Phe at position 26. This codon combination is found to be present in four other cell lines where it still specifies the TR81 determinant. This suggests that the TR81 specificity is uniquely dependent on the presence of Arg at position 74. 相似文献
44.
G. Fioretti M. De Angioletti F. Masciangelo G. Lacerra A. Scarallo C. de Bonis L. Pagano E. Guarino L. De Rosa F. Salvati C. Carestia 《American journal of human genetics》1992,50(4):781-786
Forty-three hybrid delta-beta-globin genes were characterized by DNA sequence analysis and associated RFLP haplotypes in 40 families from Abruzzo and Campania, which are on the east and west coast of Italy, respectively. All the genes had the delta-globin sequence up to the exon 2 codon 87 and had the beta-globin sequence from IVS-2-8; between these two ends, they had 58 bp in common with the delta- and beta-globin genes. Thus, they were all of the Lepore-Boston type. A chromosomal background heterogeneity was present among the mutant genes. In fact, they were all associated with (+ - - - -) 5' subhaplotype, but 23/31 from Campania were associated with (+ +) 3' subhaplotype, whereas 12/12 genes from Abruzzo and 8/31 from Campania were associated with (+ -). DNA sequencing of homozygous subjects showed that (+ +) 3' subhaplotype was associated, at IVS-2-74, with G, while (+ -) was associated with T; that is they were associated with the beta-globin gene sequence of frameworks 1 and 2, respectively. The molecular characteristics of this heterogeneity, as well as its geographical patterns in the eastern and western regions of Italy, represent strong evidence for the recurrent and multicentric origins of the mutation. 相似文献
45.
46.
M. Aluja J. Guillen P. Liedo M. Cabrera E. Rios G. de la Rosa H. Celedonio D. Mota 《BioControl》1990,35(1):39-48
A total of 1,302 parasitoids representing 8 species and 4 families were recovered from 9,818 fruit fly host fruits sampled.
The most common parasitoid species wasDiachasmimorpha longicaudata (Ashmead). Average percent parasitism ranged between 0.44 and 29.23%. Parasitoid emergence data indicate thatAnastrepha ludens (Loew),A. obliqua (Sein),A. serpentina (Wiedeman),A. striata (Schiner) andToxotrypana curvicauda (Gerstaecker) were subject to parasitism. We provide information on the population fluctuation ofAnastrepha ludens, A. obliqua, A. serpentina, A. distincta (Greene),A. striata, A. fraterculus (Wiedeman),A. chiclayae (Greene),A. montei (Costa Lima),A. leptozona (Hendel) andA. tripunctata (Wulp).Anastrepha ludens andA. obliqua were the most common species, representing 95.3% of all fruit fly species caught in McPhail traps.
相似文献
47.
48.
Isabel Allona Carmen Collada Rosa Casado Javier Paz-Ares Cipriano Aragoncillo 《Plant molecular biology》1996,32(6):1171-1176
Ch3, an endochitinase of 32 kDa present in Castanea sativa cotyledons, showed in vitro antifungal properties when assayed against Trichoderma viride. The characterization of a cDNA clone corresponding to this protein indicated that Ch3 is a class Ib endochitinase that is synthesized as a preprotein with a signal sequence preceding the mature polypeptide. Bacterial expression of mature Ch3 fused to the leader peptide of the periplasmic protein ompT resulted in active Ch3 enzyme. A plate assay was adapted for semi-quantitative determination of chitinase activity secreted from cultured bacteria, which should facilitate the identification of mutants with altered capacity to hydrolyse chitin. 相似文献
49.
A new general equation simulating irreversible DSC transitions of multimeric proteins was developed. The equation put forward here is the result of an improved mathematical re-elaboration of the classical Lumry-Eyring models, where no restrictive a priori assumptions are made on the kinetic constraints of the denaturation process, or on the enthalpy of the final denatured state. In order to test the wide applicability of this new effective theoretical tool, a series of DSC transitions were simulated with the aim of determining the effects of all relevant thermodynamic, kinetic or experimental parameters on the shape of DSC profiles. Moreover, the classical equations used widely in DSC investigations for the calculus in both kinetic parameters and changes of molecularity, were studied in the light of the model developed here, highlighting, in each case, their rather limited applicability. The new approach proposed in this article was applied to study the thermal denaturation of an hexameric protein (Glucosamine-6-phosphate deaminase), putting in evidence the practical applicability of the theoretical equations developed. 相似文献
50.