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排序方式: 共有136条查询结果,搜索用时 15 毫秒
131.
M A Gorbunov N M Nikitiuk Iu F Solov'ev L G Karpovich T V Kalashnikova A R Re?zis V A Anan'ev N V Doroshenko M A Donets M O Favorov 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》1991,(11):43-45
The results of the controlled field trial of lyophilized erythrocytic immunoglobulin diagnosticum for the detection of hepatitis A virus antigen in the urine and feces of patients are presented. This diagnosticum was used for the study of urine and fecal samples from 225 patients (of these, 176 had hepatitis A) and 54 healthy persons in the passive hemagglutination (PHA) test. Their blood sera were studied in the PHA test (to detect HBsAg) and the radioimmunoassay (to detect anti-HAV IgM). The immunoglobulin diagnosticum under study was found to be nonspecific and faintly sensitive and, therefore, unsuitable for use in medical practice. 相似文献
132.
M Iu Vasil'ev V V Vorsanova V V Kalashnikova Iu S Tatarinov 《Biulleten' eksperimental'no? biologii i meditsiny》1980,89(2):176-179
Embryonic prealbumin (EPA) was found in human cultured fibroblasts by means of immunodiffusion and immunofluorescence. No quantitative or qualitative differences in the content and localization of this antigen were revealed in embryonic and adult fibroblasts. A conclusion has been made that EPA is the product of human cultured fibroblasts and can be used as a marker of this type of cells. 相似文献
133.
A N Klimov A A Nikiforova V M Pleskov A A Kuz'min N N Kalashnikova 《Biokhimii?a (Moscow, Russia)》1989,54(1):118-123
The role of high density lipoproteins (HDL), their subfractions (HDL2 and HDL3) and lecithin: cholesterol acyltransferase (LCAT) on peroxidative modification of low density lipoproteins (LDL) in vitro was studied. Peroxidative modification was estimated by the formation of malonic dialdehyde (MDA) and LDL aggregates during LDL incubation at 37 degrees C for several days without Fe2+ or for 2 hours in the presence of Fe2+ in EDTA-free media. It was shown that the addition of HDL3 (but not HDL2) markedly decreases the formation of both MDA and LDL aggregates. Since LCAT is bound to HDL3, its effect was examined. An addition of LCAT isolated from human plasma (650-fold purification) at a concentration of 450 micrograms/ml resulted in a complete inhibition of LDL peroxidation and LDL aggregate formation. Heat-inactivated LCAT had no effect. Possible mechanisms of the protective effect of LCAT on LDL peroxidative modification are discussed. 相似文献
134.
M M Kalashnikova O V Smirnova 《Biulleten' eksperimental'no? biologii i meditsiny》1991,112(9):326-328
It was established that the hepatocytes of least shrew (Sorex minutissimus) contained 1-2 nucleus with wide nuclear pores and big nucleolus. There are abundant mitochondria with numerous crists in their matrix (many organelles were divided along the crists). Each mitochondrion was surrounded by the cistern of granular endoplasmic reticulum. The ultrastructure of Golgi complex (1-2 flat cisterns, small vesicles] indicated that the bile was secreted often, by small portions, that was the adaptation to repeated fractional feeding. There were many peroxisomes in the cytoplasm of hepatocytes. Lipid droplets were absent, glycogen granules were not at all hepatocytes. Sinusoidal cells had the usual structure. All sinusoids were open and consisted of the erythrocytes, fragments of hepatocyte cytoplasm and myelin figures in its lumen. 相似文献
135.
N. V. Kalashnikova V. G. Motin B. I. Khodorov 《Biochemistry (Moscow) Supplemental Series A: Membrane and Cell Biology》2009,3(2):206-215
The mechanisms of activity-dependent modulation of burst discharges in rat hippocampal slices have been studied. The extracellular
registration of field responses (population spike, PS, and field excitatory postsynaptic potential, fEPSP) induced by repetitive
electrical stimulation (1–4 Hz) of Schaffer collaterals (with 30 pulses trains separated by 5-min resting intervals) was performed
in cellular and dendritic layers of CA1 area. It has been established that repetitive orthodromic stimulation exerts biphasic
modulation of burst discharges in Mg2+-free medium: use-dependent potentiation (UDP) and use-dependent inhibition (UDI). The former was manifested as an increase
in the number of PS in the burst discharge associated with a corresponding lengthening of the fEPSP. During the UDI development
the number of NMDA-dependent PS in the burst was diminished despite the continuing increase in the fEPSP duration. In some
cases UDI was followed by spreading depression. Both UDP and UDI were reversible. The development of UDP and UDI could be
effectively suppressed either by the NMDA antagonists or by the GABAergic inhibition enhancer, diazepam. The picrotoxin (PTX)-induced
burst discharges did not undergo either UDP or UDI development. However, removal of Mg2+ from PTX-containing solution during continuing repetitive stimulation led to the appearance of NMDA-dependent UDI. Analysis
of the data obtained indicates that: (1) UDP results from a progressive decrease in GABA-mediated inhibition in the course
of low-frequency (1–4 Hz) repetitive stimulation (the so-called “fatigue of synaptic inhibition”); (2) UDI is caused by excessive
Ca2+ influx into the neurons due to overactivation of NMDA receptors.
The article is published in the original. 相似文献
136.
E A Kalashnikova L A Pukhal'ski? L A Pevnitski? 《Biulleten' eksperimental'no? biologii i meditsiny》1988,106(9):334-336
A positive correlation between the level of lymphocyte PHA-stimulation and the degree of stimulation inhibition by dexamethasone has been established. Using regression analysis methods, healthy volunteers were divided into three groups: sensitive, intermediate and resistant. The frequency of HLA-B7, B12 and DR2 antigens was increased in the latter group. 相似文献