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191.
Tobias Kuemmerle Patrick Hostert Volker C. Radeloff Sebastian van der Linden Kajetan Perzanowski Ivan Kruhlov 《Ecosystems》2008,11(4):614-628
Agricultural areas are declining in many areas of the world, often because socio-economic and political changes make agriculture
less profitable. The transition from centralized to market-oriented economies in Eastern Europe and the former Soviet Union
after 1989 represented major economic and political changes, yet the resulting rates and spatial pattern of post-socialist
farmland abandonment remain largely unknown. Remote sensing offers unique opportunities to map farmland abandonment, but automated
assessments are challenging because phenology and crop types often vary substantially. We developed a change detection method
based on support vector machines (SVM) to map farmland abandonment in the border triangle of Poland, Slovakia, and Ukraine
in the Carpathians from Landsat TM/ETM+ images from 1986, 1988, and 2000. Our SVM-based approach yielded an accurate change
map (overall accuracy = 90.9%; kappa = 0.82), underpinning the potential of SVM to map complex land-use change processes such
as farmland abandonment. Farmland abandonment was widespread in the study area (16.1% of the farmland used in socialist times),
likely due to decreasing profitability of agriculture after 1989. We also found substantial differences in abandonment among
the countries (13.9% in Poland, 20.7% in Slovakia, and 13.3% in Ukraine), and between previously collectivized farmland and
farmland that remained private during socialism in Poland. These differences are likely due to differences in socialist land
ownership patterns, post-socialist land reform strategies, and rural population density. 相似文献
192.
Felix Breyer Anetta Hrtlova Teresa Thurston Helen R Flynn Probir Chakravarty Julia Janzen Julien Peltier Tiaan Heunis Ambrosius P Snijders Matthias Trost Steven C Ley 《The EMBO journal》2021,40(10)
Tumour progression locus 2 (TPL‐2) kinase mediates Toll‐like receptor (TLR) activation of ERK1/2 and p38α MAP kinases in myeloid cells to modulate expression of key cytokines in innate immunity. This study identified a novel MAP kinase‐independent regulatory function for TPL‐2 in phagosome maturation, an essential process for killing of phagocytosed microbes. TPL‐2 catalytic activity was demonstrated to induce phagosome acidification and proteolysis in primary mouse and human macrophages following uptake of latex beads. Quantitative proteomics revealed that blocking TPL‐2 catalytic activity significantly altered the protein composition of phagosomes, particularly reducing the abundance of V‐ATPase proton pump subunits. Furthermore, TPL‐2 stimulated the phosphorylation of DMXL1, a regulator of V‐ATPases, to induce V‐ATPase assembly and phagosome acidification. Consistent with these results, TPL‐2 catalytic activity was required for phagosome acidification and the efficient killing of Staphylococcus aureus and Citrobacter rodentium following phagocytic uptake by macrophages. TPL‐2 therefore controls innate immune responses of macrophages to bacteria via V‐ATPase induction of phagosome maturation. 相似文献
193.
194.
Isolation of a photoactive photosynthetic reaction center-core antenna complex from Heliobacillus mobilis 总被引:4,自引:0,他引:4
A photoactive reaction center-core antenna complex was isolated from the photosynthetic bacterium Heliobacillus mobilis by extraction of membranes with Deriphat 160c followed by differential centrifugation and sucrose density gradient ultracentrifugation. The purified complex contained a Mr 47,000 polypeptide(s) that bound both the primary donor (P800) and approximately 24 antenna bacteriochlorophylls g. Time-resolved fluorescence emission spectroscopy indicated that the antenna bacteriochlorophylls g are active in energy transfer to P800, exhibiting a decay time of 25 ps. The complex contained 1.4 menaquinones, 9 Fe, and 3 labile S2- per P800. The complex was photoactive with an exponential decay time of 14 ms for P800+ yet showed no EPR-detectable Fe-S center signal in the g less than or equal to 2.0 region, either by chemical reduction to -600 mV or by illumination of reduced samples. The complex is similar to photosystem I of oxygen-evolving photosynthetic systems in that both the primary donor and a core antenna are bound to the same pigment-protein complex. 相似文献
195.
Falk Schrödl Andrea Trost Clemens Strohmaier Barbara Bogner Christian Runge Alexandra Kaser-Eichberger Sebastien Couillard-Despres Ludwig Aigner Herbert A. Reitsamer 《Cell and tissue research》2014,356(1):1-8
Pericytes are contractile cells that surround blood vessels. When contracting, they change the diameter of the vessel and therefore influence blood flow homeostasis; however, mechanisms controlling pericyte action are less well understood. Since blood flow regulation per se is controlled by the autonomic nervous system, the latter might also be involved in pericyte action. Hence, rat choroidal pericytes were analyzed for such a connection by using appropriate markers. Rat choroidal wholemounts and sections were prepared for immunohistochemistry of the pericyte marker chondroitin-sulfate-proteoglycan (NG2) and the pan-neuronal marker PGP9.5 or of tyrosine hydroxylase (TH), vasoactive intestinal polypeptide (VIP) and choline acetyl transferase (ChAT). Additionally, PGP9.5 and TH were analyzed in the choroid of DCX-dsRed2 transgenic rats, displaying red-fluorescent perivascular cells and serving as a putative model for studying pericyte function in vivo. Confocal laser-scanning microscopy revealed NG2-immunoreactive cells and processes surrounding the blood vessels. These NG2-positive cells were not co-localized with PGP9.5 but received close appositions of PGP9.5-, TH-, VIP- and ChAT-immunoreactive boutons and fibers. In the DCX-dsRed2 transgenic rat, PGP9.5 and TH were also densely apposed on the dsRed-positive cells adjacent to blood vessels. These cells were likewise immunoreactive for NG2, suggesting their pericyte identity. In addition to the innervation of vascular smooth muscle cells, the close relationship of PGP9.5 and further sympathetic (TH) and parasympathetic (VIP, ChAT) nerve fibers on NG2-positive pericytes indicated an additional target of the autonomic nervous system for choroidal blood flow regulation. Similar findings in the DCX-dsRed transgenic rat indicate the potential use of this animal model for in vivo experiments revealing the role of pericytes in blood flow regulation. 相似文献