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891.
Mangeat B Cavagliotti L Lehmann M Gers-Huber G Kaur I Thomas Y Kaiser L Piguet V 《The Journal of biological chemistry》2012,287(26):22015-22029
Influenza virus infections lead to a burst of type I interferon (IFN) in the human respiratory tract, which most probably accounts for a rapid control of the virus. Although in mice, IFN-induced Mx1 factor mediates a major part of this response, the situation is less clear in humans. Interestingly, a recently identified IFN-induced cellular protein, tetherin (also known as CD317, BST-2, or HM1.24), exerts potent antiviral activity against a broad range of retroviruses, as well as several other enveloped viruses, by impeding the release of newly generated viral particles from the cell surface. Here we show that influenza virus belongs to the targets of this potent antiviral factor. Ectopic expression of tetherin strongly inhibited fully replicative influenza virus. In addition, depleting endogenous tetherin increased viral production of influenza virions, both in cells constitutively expressing tetherin and upon its induction by IFN. We further demonstrate, by biochemical and morphological means, that tetherin exerts its antiviral action by tethering newly budded viral particles, a mechanism similar to the one that operates against HIV-1. In addition, we determined that the magnitude of tetherin antiviral activity is comparable with or higher than the one of several previously identified anti-influenza cellular factors, such as MxA, ADAR1, ISG15, and viperin. Finally, we demonstrate that influenza virus reduces the impact of tetherin-mediated restriction on its replication by several mechanisms. First, the influenza virus NS1 protein impedes IFN-mediated tetherin induction. Second, influenza infection leads to a decrease of tetherin steady state levels, and the neuraminidase surface protein partly counteracts its activity. Overall, our study helps to delineate the intricate molecular battle taking place between influenza virus and its host cells. 相似文献
892.
Markus Albert Bettina Kaiser Sander van der Krol Ralf Kaldenhoff 《Plant signaling & behavior》2010,5(9):1144-1146
The plant parasite Cuscuta reflexa induces various responses in compatible and incompatible host plants. The visual reactions of both types of host plants including obvious morphological changes require the recognition of Cuscuta ssp. A consequently initiated signaling cascade is triggered which leads to a tolerance of the infection or, in the case of some incompatible host plants, to resistance. Calcium (Ca2+) release is the major second messenger during signal transduction. Therefore, we have studied Ca2+ spiking in tomato and tobacco during infection with C. reflexa. In our recently published study1 Ca2+ signals were monitored as bioluminescence in aequorin-expressing tomato plants after the onset of C. reflexa infestation. Signals at the attachment sites were observed from 30 to 48 h after infection. In an assay with leaf disks of aequorin-expressing tomato which were treated with different C. reflexa plant extracts it turned out that the substance that induced Ca2+ release in the host plant was closely linked to the parasite''s haustoria.Key words: cuscut, odder, calcium signaling, plant parasitismThe genera Cuscuta, also known as dodder, includes 170 parasitic species with a worldwide distribution. Members of Cuscuta ssp. belong to the 1% of angiospermic plants that live as holoparasites and depend on nutrients, water and carbohydrates from other host plants.2 Cuscuta spp. lack roots or leaves but possess specific penetrating organs, the so called haustoria, which are fully developed 5–6 days after the first contact, when an interaction between parasite and host is established.As for all dicotyledonous plants, the typical Cuscuta spp. life cycle starts with the germination of seeds. At the stage of a rootless seedling, Cuscuta ssp. has just a few days to find and successfully invade a host plant. Although Cuscuta ssp. seedlings appear to coil indiscriminately around any vertical elongated object, they seem to have an efficient “sense of smell” to recognize potential “victims” and are therefore able to infest host plants more rapidly and efficiently.3 As soon as a host is reachable, Cuscuta ssp. starts to wind around the host shoot and initiates the attachment process as well as the development of haustoria.2,4 Already at this initial phase of infection (12–48 h post attachment) the host plant senses the parasite and initiates an onset of several signals which are only partially known. Amongst the several induced genes are for example those encoding AGPs (Arabinogalactan Proteins), proteins promoting the parasite''s adherence.5 Also proteins are produced which might be important for nutrient and water uptake6 or which modify the host cell wall.7In this addendum article, we focus on signals which occur in host plants within the early infection stage prior to a vascular bundle connection and refer to our article about Ca2+ signalling in C. reflexa infected tomato plants.8 Besides phytohormones or other initial signalling molecules, such cellular calcium signals might be involved in controlling the expression of important genes for developmental or resistance related processes.In our approach, Cuscuta reflexa shoots of ∼25 cm length were wrapped around transgenic constitutively aeqourin-expressing tomato (Solanum lycopersicum) and tobacco (Nicotiana benthamiana) plants. With a highly sensitive ccd-camera we then monitored the two interacting organisms. The Ca2+-signals which are released by the host-plant could be detected as light-emission. The first cytosolic calcium signals were observed 24–48 h after the parasite attachment when the haustoria formation was already initiated. Light, indicating a cytosolic calcium influx was clearly visible directly where the parasite started to penetrate host tissue via its haustoria (Fig. 1) and often appeared several times within 1–6 h. As the light signals per recorded picture were collected for 10 min it is not clear if the duration of such cytosolic calcium influx comprises 10 ms or 10 min. An additional experiment in our study was the usage of a Cuscuta reflexa haustorium extract which was applied to aequorin expressing tomato leaf discs. Here it turned out that the Ca2+-ion influx happened steadily and slowly, because signals were only visible when summed up from 2 h recording. The finding that both boiled haustoria extract and control extract, made from Cuscuta reflexa shoots without haustoria, are inactive, suggests that a protein which is expressed during the infection process might be the direct or indirect trigger of such Ca2+-signals. These results overcome furthermore the theory that Calcium signals are induced by pressure, which might also be a step during Cuscuta ssp. infection.Open in a separate windowFigure 1Cuscuta reflexa infection induces calcium-signals in aequorin-expressing tomato. Left: Bright field; middle: light emission representing Ca2+-signals at the infection site ∼30 h post onset of the parasite; signals were monitored with a ccd camera. Right: overlay.The fact that calcium fluxes act as a second messenger in several stress responses such as cold shock, wind, touch, osmotic stress,9 phytohormone signalling pathways,10 plant—symbiotic interactions10–12 or also plant pathogen interaction13–15 complicates the interpretation of the signals that are induced by Cuscuta reflexa. One possibility could be that visible Ca2+-signals are part of a signalling pathway where also SA (salicylic acid) or/and JA (jasmonic acid) play an important role. Recently, Runyon et al.16 could show that tomato plants infected with Cuscuta pentagona respond with a strong induction of JA and SA 24–36 h post infections. This time frame correlates with our described calcium signals and it has been previously described that calcium fluxes might be a part of the JA- and SA-signalling cascade.The tomato—dodder interaction, however, represents an exception among dicotyledonous plants because tomato generates a hypersensitive response which is part of a successful resistance reaction.7,16 In this particular case characteristic components of C. reflexa must be sensed by its host plant. These factors indicate “non-self” for the host plant, probably following a model comparable to the MAMP concept where characteristic molecular patterns of a pathogen are recognized in host plants via pattern recognition receptors and subsequently trigger defence responses.17,18 But sensing and signalling in host plants takes place not only in the case of an “incompatible” interaction. The developmental phenomenons of a dodder—plant interaction in a “compatible” interaction are nearly a miracle. In this case, the parasite is completely tolerated and achieves the attachment and the penetration of the host plant. It interferes in developmental processes and manipulates its host to develop vascular tissues, to build up chimerical cell walls and interspecific symplastic cell connections.16,17 Finally, it is connected to the host plant and starts to withdraw nutrients and carbohydrates19,20 by mimicking endogenous sinks. Such a tolerated interaction reminds of an interaction of plants with bacterial or fungal symbionts, where also Ca2+-signals have been described and well characterized.11,12 In the case of Cuscuta ssp.—host interaction a lot of further studies have to be done to discover all important steps of signalling cascades. 相似文献
893.
Background
In social insects, the queen is essential to the functioning and homeostasis of the colony. This influence has been demonstrated to be mediated through pheromone communication. However, the only social insect for which any queen pheromone has been identified is the honey bee (Apis mellifera) with its well-known queen mandibular pheromone (QMP). Although pleiotropic effects on colony regulation are accredited to the QMP, this pheromone does not trigger the full behavioral and physiological response observed in the presence of the queen, suggesting the presence of additional compounds. We tested the hypothesis of a pheromone redundancy in honey bee queens by comparing the influence of queens with and without mandibular glands on worker behavior and physiology.Results
Demandibulated queens had no detectable (E)-9-oxodec-2-enoic acid (9-ODA), the major compound in QMP, yet they controlled worker behavior (cell construction and queen retinue) and physiology (ovary inhibition) as efficiently as intact queens.Conclusions
We demonstrated that the queen uses other pheromones as powerful as QMP to control the colony. It follows that queens appear to have multiple active compounds with similar functions in the colony (pheromone redundancy). Our findings support two hypotheses in the biology of social insects: (1) that multiple semiochemicals with synonymous meaning exist in the honey bee, (2) that this extensive semiochemical vocabulary exists because it confers an evolutionary advantage to the colony. 相似文献894.
Elvira Vitu Sunghwan Kim Carolyn S. Sevier Omer Lutzky Nimrod Heldman Moran Bentzur Tamar Unger Meital Yona Chris A. Kaiser Deborah Fass 《The Journal of biological chemistry》2010,285(24):18155-18165
The sulfhydryl oxidase Ero1 oxidizes protein disulfide isomerase (PDI), which in turn catalyzes disulfide formation in proteins folding in the endoplasmic reticulum (ER). The extent to which other members of the PDI family are oxidized by Ero1 and thus contribute to net disulfide formation in the ER has been an open question. The yeast ER contains four PDI family proteins with at least one potential redox-active cysteine pair. We monitored the direct oxidation of each redox-active site in these proteins by yeast Ero1p in vitro. In this study, we found that the Pdi1p amino-terminal domain was oxidized most rapidly compared with the other oxidoreductase active sites tested, including the Pdi1p carboxyl-terminal domain. This observation is consistent with experiments conducted in yeast cells. In particular, the amino-terminal domain of Pdi1p preferentially formed mixed disulfides with Ero1p in vivo, and we observed synthetic lethality between a temperature-sensitive Ero1p variant and mutant Pdi1p lacking the amino-terminal active-site disulfide. Thus, the amino-terminal domain of yeast Pdi1p is on a preferred pathway for oxidizing the ER thiol pool. Overall, our results provide a rank order for the tendency of yeast ER oxidoreductases to acquire disulfides from Ero1p. 相似文献
895.
Antimalarial drug resistance has nowadays reached each drug class on the market for longer than 10 years. The focus on validated,
classical targets has severe drawbacks. If resistance is arising or already present in the field, a target-based High-Throughput-Screening
(HTS) with the respective target involves the risk of identifying compounds to which field populations are also resistant.
Thus, it appears that a rewarding albeit demanding challenge for target-based drug discovery is to identify novel drug targets.
In the search for new targets for antimalarials, we have investigated the biosynthesis of hypusine, present in eukaryotic
initiation factor 5A (eIF5A). Deoxyhypusine hydroxylase (DOHH), which has recently been cloned and expressed from P. falciparum, completes the modification of eIF5A through hydroxylation. Here, we assess the present druggable data on Plasmodium DOHH and its human counterpart. Plasmodium DOHH arose from a cyanobacterial phycobilin lyase by loss of function. It has a low FASTA score of 27 to its human counterpart.
The HEAT-like repeats present in the parasite DOHH differ in number and amino acid identity from its human ortholog and might
be of considerable interest for inhibitor design. 相似文献
896.
Wilfried Kaiser Elisabeth Huguet Jér?me Casas Céline Commin David Giron 《Proceedings. Biological sciences / The Royal Society》2010,277(1692):2311-2319
The life cycles of many organisms are constrained by the seasonality of resources. This is particularly true for leaf-mining herbivorous insects that use deciduous leaves to fuel growth and reproduction even beyond leaf fall. Our results suggest that an intimate association with bacterial endosymbionts might be their way of coping with nutritional constraints to ensure successful development in an otherwise senescent environment. We show that the phytophagous leaf-mining moth Phyllonorycter blancardella (Lepidoptera) relies on bacterial endosymbionts, most likely Wolbachia, to manipulate the physiology of its host plant resulting in the ‘green-island’ phenotype—photosynthetically active green patches in otherwise senescent leaves—and to increase its fitness. Curing leaf-miners of their symbiotic partner resulted in the absence of green-island formation on leaves, increased compensatory larval feeding and higher insect mortality. Our results suggest that bacteria impact green-island induction through manipulation of cytokinin levels. This is the first time, to our knowledge, that insect bacterial endosymbionts have been associated with plant physiology. 相似文献
897.
Norwitz ER Xu S Xu J Spiryda LB Park JS Jeong KH McGee EA Kaiser UB 《The Journal of biological chemistry》2002,277(40):37469-37478
898.
1. Isolated intact spinach chloroplasts respond to changes of the sorbitol concentration of the suspending medium as near-perfect osmometers within a large range of osmotic potentials. Under isotonic conditions (=9–10 bar), their average osmotic volume is 24 m3 and the total volume 36 m3. The osmotic volume can be increased to 63 m3 by lowering the sorbitol concentration until a critical osmotic potential of =4 bar is reached. Below that value chloroplasts rupture. Between 10 bar and 4 bar, volume changes are reversible. 2. Increasing the chloroplast volume above 24 m3 causes inhibition of photosynthesis, with 50% inhibition occurring at an osmotic potential of =5–6 bar. This corresponds to an osmotic volume of 45–55 m3. Depending on the duration of hypotonic treatment, inhibition of photosynthesis is more or less reversible. 3. Between 4 and 10 bar, the chloroplast envelope exhibits a very low permeability for ferricyanide, many metabolites, and soluble stroma proteins. 4. Electron transport is not inhibited by swelling of chloroplasts. Also, the ATP/ADP-ratio remains unchanged. 5. The solute concentration in the chloroplasts appears to be optimal for photosynthesis at 10 bar. Increasing the chloroplast volume causes inhibition of photosynthesis by dilution effects. 相似文献
899.
D. K. Santra G. Singh W. J. Kaiser V. S. Gupta P. K. Ranjekar F. J. Muehlbauer 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,102(5):676-682
Genetic diversity in Ascochyta rabiei (Pass.) Labr., the causative agent of ascochyta blight of chickpea, was determined using 37 Indian, five American (USA),
three Syrian, and two Pakistani isolates. A total of 48 polymorphic RAPD markers were scored for each isolate and the data
used for cluster analysis. Most of the isolates clustered in the dendrogram essentially according to geographic origin. Based
on the two major clusters A and B, Indian isolates were grouped into two categories, type-A and type-B. Isolates of A. rabiei within the Punjab state were more diverse than isolates from other states in northwestern India. A DNA marker (ubc7561.6 kb), specific to Indian isolates was identified. This is the first report of a molecular diversity analysis of Indian isolates
of A. rabiei. The information may assist Indian chickpea breeders in the proper deployment of blight-resistant cultivars and in disease
management.
Received: 25 April 2000 / Accepted: 11 July 2000 相似文献
900.
Photosynthesis was measured in mesophyll protoplasts isolated from spinach leaves. Under high intensity illumination and in the presence of 21% O2, half-saturation of photosynthesis by CO2 required CO2 concentrations between 8 and 12 μm at different pH values of the suspending medium. Concentrations of HCO 3 - needed for half-saturation increased correspondingly with the pH of the media. The pH profile of protoplast photosynthesis was much broader than that of CO2 assimilation by isolated chloroplasts. The data indicate that leaf cells possess mechanisms to maintain considerable differences between external and internal pH over prolonged periods of time. Protoplast photosynthesis was inhibited by nitrite, acetate and bicarbonate; inhibition was more pronounced at low than at high pH and was attributed to stroma acidification. Nitrite was reduced in the light by protoplasts and chloroplasts. At pH 7.6, the apparent Km NO 2 - was about 0.6 mM for chloroplasts and 25 mM for protoplasts. Approximate permeability coefficients for NO 2 - and HNO2 were calculated from nitrite-dependent oxygen evolution at low nitrite concentrations, known nitrite or HNO2 gradients, data on the surface area of protoplasts and chloroplasts and the pH profile of nitrite inhibition of photosynthesis. The membrane potential was assumed to be-100 mV. For the chloroplast envelope, permeability coefficients were 1.5·10-3 ms-1 (HNO2) and 2·10-8 ms-1 (NO 2 - ) and for the plasmalemma 4·10-5 ms-1 (HNO2) and 5·10-10 ms-1 (NO 2 - ). The values calculated for anion penetration probably represent upper limits of permeability. The protoplasts appeared to be largely impermeable to phosphate and phosphate esters. A rapid metabolic response of cells or cellular strands to added anionic substrates such as phosphate esters as reported in the literature appears to be possible only in damaged cells. It requires the presence of open channels between the cytosol and external medium. 相似文献