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71.
72.
激光诱变选育AC10菜用大青豆的研究报告 总被引:2,自引:1,他引:1
采用有性杂交和激光红宝石辐照交替进行,经10多年的研究、试验、选育出一个蛋白质含量、脂肪含量特高的菜用大青豆-AC10。AC10菜用大青豆,系早熟、适应性广、抗逆性强、耐迟播、产量高、效益好的品种。全生育期110天、80-85天采摘青毛豆、单产鲜毛豆700公斤以上,老豆单产160公斤左右。据农业部谷物测试中心分析,蛋白质含量48.32%,脂肪含量21.36%,合计69.68%。查新结果表明,为国内 相似文献
73.
74.
构建了含有pGHcDNA的重组痘苗病毒,用ELISA证明该重组病毒在被感染的h143细胞中,可表达出猪生长激素并将之分泌到培养基中,表达量约为1.05μg/10 ̄6细胞(24h)。用定位免疫化学法进一步证明该病毒可感染小鼠并在小鼠体内表达pGHcDNA。同时还构建了含双拷贝pGHcDNA的重组痘苗病毒,并证明其pGH表达量比单拷贝重组病毒有明显提高,约为1.50μg/10 ̄6细胞(24h)。 相似文献
75.
午间强光胁迫下SOD对大豆叶片光合机构的保护作用 总被引:5,自引:0,他引:5
晴天田间大豆叶片Pn与Pr均表现出明显的日变化,Pn日变化曲线里双峰型,中午前后降低。Pr随日照强度的增加而增加,至上午11时左右达最大值,然后缓慢下降。普通空气及低氧空气中的AQY均在中午前后降低。SOD活性也有明显的日变化,最高值出现在下午16时左右。强光下喷施SOD抑制剂DDTC明显降低Pn及低氧空气中的AQY;而在低于叶片光合作用饱和光强下喷施同样浓度DDTC则对Pn及低氧空气中的AQY无明显影响。中午前后SOD活性及Pr的增加对于保护光合机构免受强光的破坏具有重要意义。 相似文献
76.
By means of an in vivo brain microdialysis, the effect of different concentrations of physostigmine on the acetylcholine level in the dialysate of rat frontal cortex was studied. Perfusion of the various degrees of physostigmine (eserine) concentration (10 nM−10 μM) into the cortex through the dialysis membrane increased the basal acetylcholine level in a dose-dependent manner. In the presence of 10 nM, 0.1 μM and 10 μM physostigmine in the perfusate, systemic treatment with scopolamine (0.5 mg/kg, i.p.) increased 200, 270 and 510%, respectively, the relative acetylcholine level in the dialysates in comparison with the corresponding basal levels, while in the absence of physostigmine the treatment increased it only 40%. From these results, it appears that perfusion of physostigmine at a variety of concentrations, changes not only the basal level of acetylcholine induced by the inhibition of acetylcholinesterase but also the relative acetylcholine output induced by systemic treatment with scopolamine. 相似文献
77.
Akira Kai Takaaki Arashida
Kenichi Hatanaka
Toshihiro AkaikeKei Matsuzaki
Tohru Mimura
Yutaro Kaneko 《Carbohydrate polymers》1994,23(4):235-239In order to elucidate the biosynthetic process of cellulose and curdlan, 13C-labeled polysaccharides were biosynthesized by Acetobacter xylinum (IFO 13693) and Agrobacterium sp. (ATCC 31749), from culture media containing
-(1-13C)glucose,
-(2-13C)glucose,
-(4-13C)glucose, or
-(6-13C)glucose as the carbon source, and their structures were determined by 13C NMR spectroscopy. The labeling was mainly found in the original position, indicating direct polymerization of introduced glucoses. In addition, the transfer of labeling from C-2 to C-1, C-3 and C-5, from C-4 to C-1, C-2 and C-3, and from C-6 to C-1 was found in celluloses. In curdlan, the transfer of labeling from C-1 to C-3, from C-2 to C-1 and C-3, from C-4 to C-1, C-2 and C-3, and from C-6 to C-1 and C-3 was observed. From analysis of this labeling, the biosynthetic process of cellulose and curdlan was explained as involving six routes. The percentages of each route via which cellulose or curdlan is biosynthesized were estimated for upper (C-1 to C-3) and lower portions (C-4 to C-6) of glucosidic units in the polysaccharides. It is noted that very few polysaccharides are formed via the Embden-Meyerhof pathway. The lower half (C-4 to C-6) structure of introduced glucoses is well preserved in the polysaccharides. 相似文献
78.
Repeated polyketide synthase modules involved in the biosynthesis of a heptaene macrolide by Streptomyces sp. FR-008 总被引:4,自引:2,他引:2
Zhihao Hu Kai Bao Xiufen Zhou Qi Zhou David A. Hopwood Tobias Kieser Zixln Deng 《Molecular microbiology》1994,14(1):163-172
Genes for biosynthesis of a Streptomyces sp. FR-008 heptaene macrolide antibiotic with antifungal and mosquito larvicidal activity were cloned in Escherichia coli using heterologous DNA probes. The cloned genes were implicated in heptaene biosynthiesis by gene replacement. The FR-008 antibiotic contains a 38-membered, poiyketide-derived macrolide ring. Southern hybridization using probes encoding domains of the type i modular erythromycin polyketide synthase (PKS) showed that the Streptomyces sp. FR-008 PKS gene cluster contains repeated sequences spanning c. 105 kb of contiguous DNA; assuming c. 5 kb for each PKS module, this is in striking agreement with the expectation for the 21-step condensation process required for synthesis of the FR-008 carbon chain. The methods developed for transformation and gene replacement in Streptomyces sp. FR-008 make it possible to genetically manipulate polyene macrolide production, and may later lead to the biosynthesis of novel polyene macrolides. 相似文献
79.
水库对投饵网箱养鱼的负荷力 总被引:35,自引:2,他引:33
本文用18个14.3m ̄3的围隔组成的围隔群,以鲤为材料,研究了水库对投饵网箱养鱼的负荷力。实验中观测了水温、透明度、pH值、溶氧、化学耗氧量、生化需氧量与非离子氨等环境因素的变化。结束时将这些变化与我国渔业水质标准相比较,查明该负荷力约为3000kg·ha ̄(-1),建议增加25-35%的安全储备,以最大载鱼量1800-2300kg·ha ̄(-1)作为推荐值。 相似文献
80.
S. Takai M. Kasama K. Yamada N. Kai N. Hirayama H. Namiki T. Taniyama 《Human genetics》1994,93(1):13-15
The human FcRI gene encodes for a highaffinity Fc receptor that plays pivotal roles in the immune response. We have used fluorescence in situ hybridization analysis to localize the FcRI gene to human chromosome 1. The human FcRI (CD64) gene has been assigned to human chromosome 1q21.2-q21.3 using R-banded human (pro)metaphase chromosomes. 相似文献