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191.
Chengjin Guo Xiaolei Zhao Xiaoman Liu Lijun Zhang Juntao Gu Xiaojuan Li Wenjing Lu Kai Xiao 《Planta》2013,237(4):1163-1178
Several phosphate transporters (PTs) that belong to the Pht2 family have been released in bioinformatics databases, but only a few members of this family have been functionally characterized. In this study, we found that wheat TaPHT2;1 shared high identity with a subset of Pht2 in diverse plants. Expression analysis revealed that TaPHT2;1 was strongly expressed in the leaves, was up-regulated by low Pi stress, and exhibited a circadian rhythmic expression pattern. TaPHT2;1–green fluorescent protein fusions in the leaves of tobacco and wheat were specifically detected in the chloroplast envelop. TaPHT2;1 complemented the Pi transporter activities in a yeast mutant with a defect in Pi uptake. Knockdown expression of TaPHT2;1 significantly reduced Pi concentration in the chloroplast under sufficient (2 mM Pi) and deficient Pi (100 μM Pi) conditions, suggesting that TaPHT2;1 is crucial in the mediation of Pi translocation from the cytosol to the chloroplast. The down-regulated expression of TaPHT2;1 resulted in reduced photosynthetic capacities, total P contents, and accumulated P amounts in plants under sufficient and deficient Pi conditions, eventually leading to worse plant growth phenotypes. The TaPHT2;1 knockdown plants exhibited pronounced decrease in accumulated phosphorus in sufficient and deficient Pi conditions, suggesting that TaPHT2;1 is an important factor to associate with a distinct P signaling that up-regulates other PT members to control Pi acquisition and translocation within plants. Therefore, TaPHT2;1 is a key member of the Pht2 family involved in Pi translocation, and that it can function in the improvement of phosphorus usage efficiency in wheat. 相似文献
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194.
The first checklist of black fungus gnats is established; new faunistic records of Sciaridae are presented, providing a list of 10 genera and 55 species. Forty-eight species are newly listed for Morocco, increasing the total of Sciaridae known from Morocco to 69 species, belonging to 12 genera, of which six (Austrosciara Schmitz & Mjöberg, 1924, Bradysiopsis Tuomikoski, 1960, Epidapus Haliday, 1851, Lycoriella Frey, 1942, Pseudolycoriella Menzel & Mohrig, 1998 and Sciara Meigen, 1803) are newly reported for Moroccan fauna. 相似文献
195.
Bao‐Kai Cui 《Nordic Journal of Botany》2013,31(3):326-330
Two new polypores (Ceriporiopsis lavendula B. K. Cui sp. nov. and Skeletocutis inflata B. K. Cui sp. nov.) are described from the Guangdong Province in southern China. Ceriporiopsis lavendula is characterized by having resupinate basidiocarps with lavender to grayish blue pore surface and very thin to almost absent subiculum, a monomitic hyphal system with clamped generative hyphae, and oblong‐ellipsoid to ellipsoid basidiospores. Moreover, it grows on wood of Castanopsis exclusively. Skeletocutis inflata is characterized by having pileate basidiocarps, smaller pores (8–10 per mm), inflated and partly dissolved skeletal hyphae in KOH. In addition, its tramal skeletal hyphae are parallel along the tubes. Identification keys to species of Ceriporiopsis and Skeletocutis occurring in China are provided. 相似文献
196.
Tyler Schwend Zhigang Jin Kai Jiang Brian J. Mitchell Jianhang Jia Jing Yang 《The Journal of biological chemistry》2013,288(45):32809-32820
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198.
Christopher Cottingham Roujian Lu Kai Jiao Qin Wang 《The Journal of biological chemistry》2013,288(40):29193-29205
Inter-regulation of adrenergic receptors (ARs) via cross-talk is a long appreciated but mechanistically unclear physiological phenomenon. Evidence from the AR literature and our own extensive studies on regulation of α2AARs by the scaffolding protein spinophilin have illuminated a potential novel mechanism for cross-talk from β to α2ARs. In the present study, we have characterized a mode of endogenous AR cross-talk in native adrenergic neurons whereby canonical βAR-mediated signaling modulates spinophilin-regulated α2AAR endocytosis through PKA. Our findings demonstrate that co-activation of β and α2AARs, either by application of endogenous agonist or by simultaneous stimulation with distinct selective agonists, results in acceleration of endogenous α2AAR endocytosis in native neurons. We show that receptor-independent PKA activation by forskolin is sufficient to accelerate α2AAR endocytosis and that α2AAR stimulation alone drives accelerated endocytosis in spinophilin-null neurons. Endocytic response acceleration by β/α2AAR co-activation is blocked by PKA inhibition and lost in spinophilin-null neurons, consistent with our previous finding that spinophilin is a substrate for phosphorylation by PKA that disrupts its interaction with α2AARs. Importantly, we show that α2AR agonist-mediated α2AAR/spinophilin interaction is blocked by βAR co-activation in a PKA-dependent fashion. We therefore propose a novel mechanism for cross-talk from β to α2ARs, whereby canonical βAR-mediated signaling coupled to PKA activation results in phosphorylation of spinophilin, disrupting its interaction with α2AARs and accelerating α2AAR endocytic responses. This mechanism of cross-talk has significant implications for endogenous adrenergic physiology and for therapeutic targeting of β and α2AARs. 相似文献
199.
Tarsha Ward Ming Wang Xing Liu Zhikai Wang Peng Xia Youjun Chu Xiwei Wang Lifang Liu Kai Jiang Huijuan Yu Maomao Yan Jianyu Wang Donald L. Hill Yuejia Huang Tongge Zhu Xuebiao Yao 《The Journal of biological chemistry》2013,288(22):15771-15785
The microtubule cytoskeleton network orchestrates cellular dynamics and chromosome stability in mitosis. Although tubulin acetylation is essential for cellular plasticity, it has remained elusive how kinetochore microtubule plus-end dynamics are regulated by p300/CBP-associated factor (PCAF) acetylation in mitosis. Here, we demonstrate that the plus-end tracking protein, TIP150, regulates dynamic kinetochore-microtubule attachments by promoting the stability of spindle microtubule plus-ends. Suppression of TIP150 by siRNA results in metaphase alignment delays and perturbations in chromosome biorientation. TIP150 is a tetramer that binds an end-binding protein (EB1) dimer through the C-terminal domains, and overexpression of the C-terminal TIP150 or disruption of the TIP150-EB1 interface by a membrane-permeable peptide perturbs chromosome segregation. Acetylation of EB1-PCAF regulates the TIP150 interaction, and persistent acetylation perturbs EB1-TIP150 interaction and accurate metaphase alignment, resulting in spindle checkpoint activation. Suppression of the mitotic checkpoint serine/threonine protein kinase, BubR1, overrides mitotic arrest induced by impaired EB1-TIP150 interaction, but cells exhibit whole chromosome aneuploidy. Thus, the results identify a mechanism by which the TIP150-EB1 interaction governs kinetochore microtubule plus-end plasticity and establish that the temporal control of the TIP150-EB1 interaction by PCAF acetylation ensures chromosome stability in mitosis. 相似文献
200.
Zhong‐Chao Li Qian‐Mei Zhang Kai‐Ming Liang Wan‐Hui Ye Zhang‐Ming Wang 《Biological journal of the Linnean Society. Linnean Society of London》2013,109(4):747-756
The local spatial genetic structures of cave‐associated plants are seldom studied. Given that these plants are mainly confined to small areas in and around the entrances of caves, we hypothesized that they might lack genetic structures at local scales. To test this hypothesis, we sampled two large populations (named D and T) of a critically endangered perennial herb, Primulina tabacum, which is endemic to karst caves in southern China. We analysed nine microsatellite loci and sequenced four chloroplast DNA (cpDNA) intergenic spacer regions to study the genetic diversity and structure within and between both populations. Both populations have distinct genetic characteristics. Samples from two subpopulations in population D showed considerable genetic divergence. This is not consistent with the hypothesis that P. tabacum has a weak genetic structure at a local scale. However, 94% of the individuals in population T shared the same multilocus genotype, which indicates little genetic structure within this population. The contributions of seed flow, pollen flow and (sub)population history to the genetic diversity and structure in each and both populations are discussed. Our study is the first to investigate local genetic diversity and structure in a cave‐associated plant, and provides valuable information for the sustainable conservation of such species. © 2013 The Linnean Society of London, Biological Journal of the Linnean Society, 2013, 109 , 747–756. 相似文献