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91.
Derivation,characterization and expansion of fetal chondrocytes on different microcarriers 总被引:1,自引:0,他引:1
Fetal chondrocytes (FCs) have recently been identified as an alternative cell source for cartilage tissue engineering applications
because of their partially chondrogenically differentiated phenotype and developmental plasticity. In this study, chondrocytes
derived from fetal bovine cartilage were characterized and then cultured on commercially available Cytodex-1 and Biosilon
microcarriers and thermosensitive poly(hydroxyethylmethacrylate)-poly(N-isopropylacrylamide) (PHEMA-PNIPAAm) beads produced
by us. Growth kinetics of FCs were estimated by means of specific growth rate and metabolic activity assay. Cell detachment
from thermosensitive microcarriers was induced by cold treatment at 4 °C for 20 min or enzymatic treatment was applied for
the detachment of cells from Cytodex-1 and Biosilon. Although attachment efficiency and proliferation of FCs on PHEMA-PNIPAAm
beads were lower than that of commercial Cytodex-1 and Biosilon microcarriers, these beads also supported growth of FCs. Detached
cells from thermosensitive beads by cold induction exhibited a normal proliferative activity. Our results indicated that Cytodex-1
microcarrier was the most suitable material for the production of FCs in high capacity, however, ‘thermosensitive microcarrier
model’ could be considered as an attractive solution to the process scale up for cartilage tissue engineering by improving
surface characteristics of PHEMA-PNIPAAm beads. 相似文献
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Mustafa Kahraman Emel Bıçakçı Ordu Koray Yeşiladalı Candan Tamerler 《Preparative biochemistry & biotechnology》2013,43(6):507-519
NAD+-dependent formate dehydrogenase (FDH, EC 1.2.1.2) is of use in the regeneration of NAD(P)H coenzymes, and therefore has strong potential for practical application in chemical and medical industries. A low-cost production of recombinant Escherichia coli (E. coli) containing FDH from Candida methylica (cmFDH) was optimized in molasses-based medium by using response surface methodology (RSM) based on central composite design (CCD). The beet molasses as a sole carbon source, (NH4)2HPO4 as a nitrogen and phosphorus source, KH2PO4 as a buffer agent, and Mg2SO4 · 7H2O as a magnesium and sulfur source were used as variables in the medium. The optimum medium composition was found to be 34.694 g L?1 of reducing sugar (equivalent to molasses solution), 8.536 g L?1 of (NH4)2HPO4, 3.073 g L?1 of KH2PO4, and 1.707 g L?1 of Mg2SO4 · 7H2O. Molasses-based culture medium increased the yield of cmFDH about three times compared to LB medium. The currently developed media has the potential to be used in industrial bioprocesses with low-cost production. 相似文献
94.
Age‐associated microRNA expression in human peripheral blood is associated with all‐cause mortality and age‐related traits
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Tianxiao Huan George Chen Chunyu Liu Anindya Bhattacharya Jian Rong Brian H. Chen Sudha Seshadri Kahraman Tanriverdi Jane E. Freedman Martin G. Larson Joanne M. Murabito Daniel Levy 《Aging cell》2018,17(1)
Recent studies provide evidence of correlations of DNA methylation and expression of protein‐coding genes with human aging. The relations of microRNA expression with age and age‐related clinical outcomes have not been characterized thoroughly. We explored associations of age with whole‐blood microRNA expression in 5221 adults and identified 127 microRNAs that were differentially expressed by age at P < 3.3 × 10?4 (Bonferroni‐corrected). Most microRNAs were underexpressed in older individuals. Integrative analysis of microRNA and mRNA expression revealed changes in age‐associated mRNA expression possibly driven by age‐associated microRNAs in pathways that involve RNA processing, translation, and immune function. We fitted a linear model to predict ‘microRNA age’ that incorporated expression levels of 80 microRNAs. MicroRNA age correlated modestly with predicted age from DNA methylation (r = 0.3) and mRNA expression (r = 0.2), suggesting that microRNA age may complement mRNA and epigenetic age prediction models. We used the difference between microRNA age and chronological age as a biomarker of accelerated aging (Δage) and found that Δage was associated with all‐cause mortality (hazards ratio 1.1 per year difference, P = 4.2 × 10?5 adjusted for sex and chronological age). Additionally, Δage was associated with coronary heart disease, hypertension, blood pressure, and glucose levels. In conclusion, we constructed a microRNA age prediction model based on whole‐blood microRNA expression profiling. Age‐associated microRNAs and their targets have potential utility to detect accelerated aging and to predict risks for age‐related diseases. 相似文献
95.
A. E. Kahraman T. Z. Alicli T. Akayli I. K. Oray 《Zeitschrift fur angewandte Ichthyologie》2008,24(5):551-554
The study aimed at identifying spawning season and potential year classes reaching maturity in the north‐eastern Mediterranean, an area where little information on tuna spawning is available. Gonads (60 ovaries and 36 testis) were obtained from little tunny, Euthynnus alletteratus. The fish were caught between November 2002 and May 2005 in the north‐eastern Mediterranean Sea. The ovaries were histologically examined to determine the reproductive conditions and developmental stages of oocytes. Seven females sampled in May, July, and August were sexually mature (stage III or IV). The gonado‐somatic index (GSI) indicated that spawning generally occurred between May and September. The most intensive spawning period was observed between July and August. The sex ratio was calculated as 1 : 1.7 M/F (total n = 96). The length and weight relationship was calculated with W = 0.038 L2.77, ages from year I to IV being included in the analysis. 相似文献
96.
Kahraman Gurcan Fatih Demirel Mehmet Tekin Serap Demirel Taner Akar 《BMC plant biology》2017,17(1):171
Background
Turkey is one of the important gene centers for many crop species. In this research, some ancient wheats such as tetraploid and diploid hulled wheats together with hexaploid tir wheats (Triticum aestivum ssp. leucospermum Korn.) landraces mainly adapted to harsh winter conditions of Eastern Anatolian region of Turkey were characterized at agro-morphological and molecular level. Totally 50 hulled wheat population from Kastamonu, Konya and Kayseri provinces and 15 tir wheats from Kars provinces of Turkey were in-situ collected for characterization in 2013. Some quantitative and qualitative traits of each population were determined.Results
Twenty three hulled wheat population collected from Kastamonu province were distinguished into nine emmer and 14 einkorn wheats at morphological level. Additionally, Konya, Kayseri and Kars population were characterized as einkorn, emmer and tir wheat, respectively. Among the evaluated traits, protein ratios of hulled wheats were strikingly higher than registered cultivars. All the populations were also examined by molecular level by using fluorescently labelled 11 polymorphic SSRs primers. The primers exhibited 104 bands, ranging from 6 to 16 with a mean value 9.45 per loci. The clustering analysis separated the germplasm into two clusters which were also divided into two subclusters based on genetic similarity coefficient. Sixty-five population and five checks were analyzed to estimate mean number of alleles (N), expected and observed heterozygoties (He and Ho), polymorphism information content (PIC), Wright fix index (F), genetic deviation from Hardy-Weinberg expectation (Fit-Fis) and genetic variation (Fst) were determined as 9.45, 0.71, 0.07, 0.67, 0.90, 0.39, 0.87 and 0.39, respectively. A clear genetic deviation from Hardy – Weinberg expectation was observed among population in particular. These results showed considerable genetic variation among landraces rather than within population.Conclusions
These molecular information has revealed genetically diverse einkorn, emmer wheat and tir wheat population could be used as parents for further breeding studies in both Turkey and abroad. Furthermore, the molecular analysis has also generally discriminated the germplasm into ploidy level.97.
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OBJECTIVE: Graves' disease (GD) with sarcoid involvement of the thyroid gland has rarely been reported. METHOD: We report a case of GD with thyroid sarcoidosis in a 28-year-old woman. Thyroid function was assessed by triiodothyronine (T(3)), thyroxine (T(4)), thyroid-stimulating hormone (TSH) and TSH receptor antibodies (TSH-R Ab). Thyroid scintigraphy, ultrasound and fine-needle aspiration biopsy were performed. The patient underwent surgery. RESULT: The patient had a nodular goiter. Serum T(3), T(4) and TSH-R Ab levels were elevated with suppressed TSH level. Scintigraphy showed diffuse activity as seen in GD, and ultrasound revealed that parenchyma was heterogenous. Sarcoidosis was discovered on routine chest X-ray. Although no sarcoid involvement was found on specimen, the thyroid gland showed non-caseating granulomas on histology. CONCLUSION: Since sarcoid involvement of the thyroid gland can cause hypofunction, we report the uncommon infiltration of sarcoidosis with hyperthyroidism. 相似文献
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Carsten Eggers David J. Pedrosa Deniz Kahraman Franziska Maier Catharine J. Lewis Gereon R. Fink Matthias Schmidt Lars Timmermann 《PloS one》2012,7(10)