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501.
Effects of disturbance scale on soil microbial communities in the Western Cascades of Oregon 总被引:1,自引:0,他引:1
Stacie A. Kageyama Nancy R. Posavatz Sarah S. Jones Kirk E. Waterstripe Peter J. Bottomley Kermit Cromack Jr. David D. Myrold 《Plant and Soil》2013,372(1-2):459-471
Aims
To gain a better understanding of how rapidly microbial communities respond to different magnitudes of perturbation that mimic minor or catastrophic disturbances.Methods
Two montane sites in the western Cascade Mountains of Oregon with adjacent areas of forest and meadow vegetation were studied. A reciprocal transplant experiment evaluated both minor (soil cores remaining in the same vegetation type) or more severe disturbance (soil cores transferred to a different vegetation type). The biomass and composition of the bacterial and fungal communities were measured for 2 years following the establishment of the experiment.Results
Minor disturbance (coring) had little impact on microbial biomass but transferring between vegetation type showed greater fungal biomass in soil incubated in the forest environment. The composition of bacterial communities was not influenced by coring but responded strongly to transfers between vegetation sites, changing to reflect their new environment after 2 years. Fungal community composition responded somewhat to coring, probably from disrupting mycorrhizal fungal hyphae, but more strongly to being transferred to a new environment.Conclusions
The response of the microbial community to major disturbance was rapid, showing shifts reflective of their new environment within 2 years, suggesting that microbial communities have the capacity to quickly adjust to catastrophic disturbances. 相似文献502.
Tomohito Hirao Yoshishige Urata Kan Kageyama Midori Ikezaki Miho Kawakatsu Michiko Matsuse 《Free radical research》2013,47(11-12):957-965
Dehydroepiandrosterone (DHEA) modulates sensitivity to radiation-induced injury in human neuroglioma cells (H4) through effects on Akt signalling by glutathione (GSH)-dependent redox regulation. Previous treatment of H4 cells with DHEA for 18 h reduced the γ-ray-induced phosphorylation of Akt, activated p21waf1 synthesis and up-regulated phosphorylation of Rb independent of p53. These reactions were followed by a decrease in cell number and an increase in apoptosis and G2/M checkpoint arrest. The suppression of phosphorylation of Akt by DHEA was due to regulation of the dephosphorylation by protein phosphatase 2A (PP2A). DHEA up-regulated the expression of γ-glutamylcysteine synthetase, a rate-limiting enzyme of glutathione (GSH) synthesis, and the levels of GSH to maintain PP2A activity. The results suggested that DHEA increases the sensitivity of cells to γ-ray irradiation by inducing apoptosis and cell cycle arrest through GSH-dependent regulation of the reduced form of PP2A to down-regulate the Akt signalling pathway. 相似文献
503.
Yukiko Harima Yoshiki Takashima Yuriko Ueda Toshiyuki Ohtsuka Ryoichiro Kageyama 《Cell reports》2013,3(1):1-7
Highlights? Reduction of Hes7 intron number accelerates the tempo of Hes7 oscillation ? Reduction of Hes7 intron number accelerates the tempo of somite segmentation ? Hes7 is a key segmentation pacemaker ? The number of introns may be important for normal timing of gene expression 相似文献
504.
Rapid sex chromosomal chimerism analysis in heterosexual twin female calves by Loop-mediated Isothermal Amplification 总被引:1,自引:0,他引:1
Hirayama H Katagiri S Kageyama S Minamihashi A Moriyasu S Sawai K Onoe S Takahashi Y 《Animal reproduction science》2007,101(1-2):38-44
We attempted to apply an embryo sexing kit with Loop-mediated Isothermal Amplification (LAMP) to sex chromosomal chimerism analysis in heterosexual twin female calves. Peripheral blood was used for the amplification of male-specific DNA, derived from XY leukocytes. When blood samples were diluted 1:1000 in LAMP reaction mixture, hemoglobin or blood coagulation did not influence the turbidity measurement of the reaction mixture for detection of amplified DNA. This procedure detected the existence of XY leukocytes of 0.01% in female blood. Furthermore, all heterosexual twin female calves, bearing sex chromosomal chimerism based on karyotyping and PCR, showed male-specific DNA from peripheral blood by LAMP. These results indicated that the embryo sexing kit with LAMP was available for sensitive detection of sex chromosomal chimerism. This procedure made it possible to detect easily Y-chromosome specific DNA in a short interval compared with PCR, and was convenient for field application of freemartin diagnosis. 相似文献
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Kageyama T 《Biochemistry》2006,45(48):14415-14426
Pepsin B is known to be distributed throughout mammalia, including carnivores. In this study, the proteolytic specificity of canine pepsin B was clarified with 2 protein substrates and 37 synthetic octapeptides and compared with that of human pepsin A. Pepsin B efficiently hydrolyzed gelatin but very poorly hydrolized hemoglobin. It was active against only a group of octapeptides with Gly at P2, such as KPAGF/LRL and KPEGF/LRL (arrows indicate cleavage sites). In contrast, pepsin A hydrolyzed hemoglobin but not gelatin and showed high activity against various types of octapeptides, such as KPAEF/FRL and KPAEF/LRL. The specificity of pepsin B is unique among pepsins, and thus, the enzyme provides a suitable model for analyzing the structure and function of pepsins and related aspartic proteinases. Because Tyr13 and Phe219 in/around the S2 subsites (Glu/Ala13 and Ser219 are common in most pepsins) appeared to be involved in the specificity of pepsin B, site-directed mutagenesis was undertaken to replace large aromatic residues with small residues and vice versa. The Tyr13Ala/Phe219Ser double mutant of pepsin B was found to demonstrate broad activity against hemoglobin and various octapeptides, whereas the reverse mutant of pepsin A had significantly decreased activity. According to molecular modeling of pepsin B, Tyr13 OH narrows the substrate-binding space and a peptide with Gly at P2 might be preferentially accommodated because of its high flexibility. The hydroxyl can also make a hydrogen bond with nitrogen of a P3 residue and fix the substrate main chain to the active site, thus restricting the flexibility of the main chain and strengthening preferential accommodation of Gly at P2. The phenyl moiety of Phe219 is bulky and narrows the S2 substrate space, which also leads to a preference for Gly at P2, while lowering the catalytic activity against other peptide types without making a hydrogen-bonding network in the active site. 相似文献