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排序方式: 共有174条查询结果,搜索用时 875 毫秒
161.
Marika Pikta Timea Szanto Margus Viigimaa Sandra Lejniece Drta Balode Kadri Saks Valdas Banys 《Journal of Medical Biochemistry》2021,40(2):167
BackgroundAccurate diagnosis and classification of von Willebrand disease (VWD) are essential for optimal management. The von Willebrand factor multimers analysis (VWF:MM) is an integral part of the diagnostic process in the phenotypic classification, especially in discrepant cases. The aim of this study was to evaluate the performance of a new Hydragel 11VWF multimer assay (H11VW).MethodsAnalytical performance characteristics such as repeatability (intra-assay variability, in gel between track variation), reproducibility (inter-assay variability, between gel variation), sensitivity, EQA performance and differences between two commercially available VWF:MM kits (H5VW and H11VW) were analysed in healthy volunteers'' plasmas using in-house prepared reference plasma.ResultsRepeatability and reproducibility results of H11VW demonstrated acceptable and equivalent performance with previously verified H5VW. Participation in EQA was successful. No statistically significant difference was detected between H5VW and H11VW kits for different fractions of multimers: LMWM p=0.807; IMWM p=0.183; HMWM p=0.774.ConclusionsH11VW demonstrated acceptable analytical performance characteristics. H11VW kit conveniently offers a more significant number of samples on a single gel. H5VW and H11VW kits can be used in daily practice interchangeably. 相似文献
162.
The aim of the study was to compare the effect of two acute puerperal metritis (APM) treatment protocols on uterine condition
during the late puerperal period (5th to 7th week). Late gestation healthy cows (n = 21) were divided randomly in three equal groups. Parturitions were induced. Treatments
of APM were started on the third day postpartum (PP). Group A was treated with an oxytocin analogue carbetocin for three days
and intrauterine administration of cephapirin between days 15 and 17. Group B was given intramuscular injection of ceftiofur
for five days followed by two injections of prostaglandin F2α, at an interval of 12 h, on the eighth day PP. Group C served as the control group with no treatment. Body temperature was
recorded daily for 14 days PP. Uterine biopsies for bacteriology, and uterobrush samples for cytology, were taken once a week
from the 5th to 7th week postpartum. No differences were found in body temperature on day 14 PP, presence of bacteriological infections and disappearance
of uterine inflammatory signs diagnosed by cytological examination between experimental groups. 相似文献
163.
Setegn Worku Alemu Naveen Kumar Kadri Chad Harland Pierre Faux Carole Charlier Armando Caballero Tom Druet 《Heredity》2021,126(3):410
The estimation of the inbreeding coefficient (F) is essential for the study of inbreeding depression (ID) or for the management of populations under conservation. Several methods have been proposed to estimate the realized F using genetic markers, but it remains unclear which one should be used. Here we used whole-genome sequence data for 245 individuals from a Holstein cattle pedigree to empirically evaluate which estimators best capture homozygosity at variants causing ID, such as rare deleterious alleles or loci presenting heterozygote advantage and segregating at intermediate frequency. Estimators relying on the correlation between uniting gametes (FUNI) or on the genomic relationships (FGRM) presented the highest correlations with these variants. However, homozygosity at rare alleles remained poorly captured. A second group of estimators relying on excess homozygosity (FHOM), homozygous-by-descent segments (FHBD), runs-of-homozygosity (FROH) or on the known genealogy (FPED) was better at capturing whole-genome homozygosity, reflecting the consequences of inbreeding on all variants, and for young alleles with low to moderate frequencies (0.10 < . < 0.25). The results indicate that FUNI and FGRM might present a stronger association with ID. However, the situation might be different when recessive deleterious alleles reach higher frequencies, such as in populations with a small effective population size. For locus-specific inbreeding measures or at low marker density, the ranking of the methods can also change as FHBD makes better use of the information from neighboring markers. Finally, we confirmed that genomic measures are in general superior to pedigree-based estimates. In particular, FPED was uncorrelated with locus-specific homozygosity.Subject terms: Conservation genomics, Animal breeding, Inbreeding 相似文献
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Isolation of auxotrophic mutants and conjugal transfer of plasmids are successfully demonstrated in four obligate methylotrophic strains isolated at Kuwait Institute for Scientific Research (KISR)Out of 11 auxotrophic, mutants, three required methionine and two leucine. Complete transfer of plasmid R68.45 from Pseudomonasto KISR methylotrophs and vice versa have been achieved with a low transfer rate. 相似文献
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Mario L. Suvà Esther Rheinbay Shawn M. Gillespie Anoop P. Patel Hiroaki Wakimoto Samuel D. Rabkin Nicolo Riggi Andrew S. Chi Daniel P. Cahill Brian V. Nahed William T. Curry Robert L. Martuza Miguel N. Rivera Nikki Rossetti Simon Kasif Samantha Beik Sabah Kadri Itay Tirosh Ivo Wortman Alex K. Shalek Orit Rozenblatt-Rosen Aviv Regev David N. Louis Bradley E. Bernstein 《Cell》2014
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