Behavioural changes are related to moult regulation in the cockroach, Periplaneta americana

Abstract. Larval holometabolous insects show changes in behaviour (e.g. start of wandering and spinning) in specific periods of the moulting cycle in relation to definite ratios of juvenile hormone and moulting hormone (ecdysone). In hemimetabolous insects no such changes in behaviour are known. It should be investigated whether the cockroach Periplaneta americana shows changes in locomotor activity and in food and water consumption in relation to periods of ecdysone production during the last larval stage. Within a mean duration of the last larval stage of 30 days there were two periods of reduced locomotor activity: on day 9 and between days 13 and 17. From days 12–13 food consumption decreased by c . 40% up to the day 18. Water consumption decreased between days 9 and 18 by about 55%. Peaks of ecdysone production appeared after these changes of behaviour in each case. Therefore in larval Periplaneta ecdysone seems not to trigger these behavioural changes.     

Martinssonia elongata gen. et sp.n., a crustacean-like euarthropod from the Upper Cambrian 'Orsten' of Sweden

A tiny arthropod, with five growth stages, is described. Three of the instars are metanauplius-like larvae, having unsegmented bodies and four pairs of appendages. The largest stage, with a length of about 1.5 mm, may still be immature. Its body is divided into three tagmata. The cephalon, including five appendiculate segments, h a projecting forehead with a rostral spine and a small shield with a joint between fourth and fifth segments. Eyes are absent. The trunk is composed of seven annular segments, the anterior two with appendages. The caudal end is a long pleotelson-like segment with the anus on its ventral surface. There are seven pairs of appendages: uniramous antennulae, composed of few tubular podomeres; four pairs of biramous postantennular, almost homeomorphic cephalic appendages; two pairs on the trunk, the anterior pair being similar to the cephalic appendages except for the exopodite, the posterior being much smaller, uniramous and apparently rudimentary. Martinssonia was probably benthic, feeding on detritic particles which it stirred up from the bottom. Besides various crustacean-like features, the new form reveals structures different from Crustacea as well as from all other known arthropodan groups. Martinssonia presumably is a descendant of an euarthropodan group, originating from the crustacean branch long before reaching the eucrustacean level of evolution.     

Effects of rearing and induction temperature on the temporal dynamics of heat shock protein 70 expression in a butterfly

The temporal dynamics of heat shock protein 70 (HSP70) expression in response to longer‐term acclimation and rapid hardening in the butterfly Lycaena tityrus is investigated. After a 1‐h exposure to 1 °C or 37 °C, HSP70 is quickly up‐regulated within 1 h and down‐regulated within 2 h. The fast dynamic of HSP70 expression is in contrast to the patterns found in organisms inhabiting more stable thermal environments, and is interpreted as an adaptation to the large and rapid temperature variation experienced by flying ectotherms. HSP70 expression is higher in males than in females, as well as in animals reared at 27 °C than at 20 °C, although it is very similar across the high and low induction temperatures. Animals reared at the higher temperature, however, respond less strongly to high‐temperature stress.     

Crustacea with preserved soft parts from the Upper Cambrian of Sweden

Six monotypic new genera of small crustaceans with phosphatised integument are described from Upper Cambrian, mainly bituminous limestone of southern Sweden. These are Dala peilertae, Bredocaris admirabilis, Walossekia quinquespinosa, Rehbachiella kinnekullensis, Skara anulata and Oelandocaris oelandica. The well-preserved details reveal a nauplius-like organisation of the head region. At least in Bredocaris, Walossekia , and Rehbachiella the head tagma has only three to four specialised pairs of appendages; Skara and Oelandocaris have five pairs of headal limbs. The antennae and the mandibulae served for locomotion and for mastication. All species have a filter apparatus with a distinct filter groove and serially constructed appendages. Four of the six forms had developed a conspicuous median compound eye. Specialisation in the development of the limbs, etc., suggests that some of the forms were benthie, while others may have been epibcnthic and active swimmers, Crustacea , Dala, Bredocaris, Walossekia, Rehbachiella, Skara, Oelandocaris, soft-part preservation, Upper Cambrian, Sweden.     

Evolution of sperm morphology in potamid freshwater crabs (Crustacea: Brachyura: Potamoidea)

We investigated sperm cells and spermatophores of four species of Old World freshwater crabs belonging to three different genera of the subfamily Potaminae (family Potamidae). Characters previously believed to be apomorphic for the potamid subfamily Potamiscinae were also found to occur in the Potaminae. To infer the morphological ancestral character state combination of the Potamidae, ancestral character state analysis of four different sperm traits was performed, based on a 16S rDNA phylogeny of the investigated species. Comparing molecular phylogeny and character state distribution, several cases of convergent evolution could be identified. The densely packed, coenospermic spermatophores and the occurrence of a ‘tongue‐and‐groove’ connection between operculum and acrosomal zones are probably apomorphies for the whole Potamidae. The spermatozoa of Socotrapotamon socotrense show several unique characters. We also analysed the evolution of acrosome size. The sperm cells of the Potamidae and their sister‐group Gecarcinucidae only slightly overlap in acrosome size. Within the investigated species, the ‘East Asia’ subclade (subfamily Potamiscinae) developed significantly larger acrosomes than the subfamily Potaminae. Our results suggest that the use of brachyuran acrosome morphology for phylogenetic inference at the family level is strongly affected by small sample size, and by convergent character evolution. © 2010 The Linnean Society of London, Zoological Journal of the Linnean Society, 2010.     

The responses of central acoustic and vibratory interneurones in bushcrickets and locusts to ultrasonic stimulation

ABSTRACT. Analysis of the ultrasonic content of the calling songs of two tettigoniids, Decticus verrucivorus L. and Tettigonia cantans Fuessly, showed that the major secondary energy peaks in the ultrasonic range are only about 15–20 dB below the main audible frequency peaks. The song of the acridid, Locusta migratoria L., contains no appreciable secondary peaks at ultrasonic frequencies, Bifunctional acoustic-vibratory interneurones are present in the ventral nerve cord of all three species. They are divided into three categories, according to their response characteristics: VS (vibration and sound), S (sound) and V (vibration) neurones. All the unit-types capable of coding sound signals in the ventral cord (VS and S neurones) are sensitive to frequencies of up to 100 kHz, with one exception (S3). In tettigoniids, three of these unit-types are more sensitive at ultrasonic frequencies than they are at the audible frequencies of their conspecific songs. Among the vibratory neurones (V), one unit-type receives inhibitory inputs from ultrasonic acoustic primary receptors. The possible importance of ultrasonic perception in the natural environment is briefly discussed.     

The ultrastructure of the protonephridial system of Götte's larva of Stylochus mediterraneus (Polycladida, Platyhelminthes)

The protonephridial system of Götte's larva of Stylochus mediterraneus was studied by electron microscopy. There is one protonephridium on each side of the body, formed by one terminal and one canal cell. The terminal filtration apparatus is formed by a single cell (the terminal cell) with several globular processes, the largest of which includes the nucleus. Fingers of cytoplasm (leptotriches) from each process penetrate the lumen surrounding the bundle of cilia and fingers from adjacent processes interdigitate to form a pattern of convoluted slits which constitute the weir. The single canal cell is inserted internally to the terminal cell at the top of the weir and encloses the lumen without a junction. Septate junctions are present between the terminal and canal cells. The lumen of the canal cell is smooth-walled for most of its length and cilia arise and terminate at all levels of the terminal and canal cells. Posterior to the larval mouth opening, the canal cell crosses the epithelium and the lumen ramifies to form the excretory opening. The terminal apparatus closely resembles that found in the freshwater planarian Bdellocephala brunnea .     

The Buccal Organ of Some Monogenea Polyopisthocotylea

Light and electron microscopic studies of the 'buccal suckers' of Gotocotyla secunda showed that they are complex structures with muscular, glandular, (resorptive?), and probably sensory components. The name 'buccal organ' is therefore proposed for them. Ducts run from the lumen of the buccal organs to their dorsal, ventral and lateral surface and communicate with lacunae surrounding them. The surface of the ducts and of the muscular walls of the buccal organs are lined by tegument with short microvilli, tegument with dense, long surface lamellae, and tegument with thick, long microvilli which have an electron-dense core. The latter type of tegument contains many vesicles and electrondense ovoid bodies, and is traversed by gland ducts containing large secretory droplets. Electron microscopic examination showed that the prebuccal convoluted structures in Heteromicrocotyloides mirabilis are a modified tegument connected to subtegumental cells. Light microscopic studies of Gotocotyla bivaginalis. Pseudothoracocotyla indica, Pricea multae and Heteromicrocotyla australiensis showed that the first three species have buccal organs similar to those in G. secunda , whereas the last species resembles H. mirabilis. It is tentatively assumed that the buccal organs play a role in finding suitable sites for bloodfeeding.