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Jae Chul SHIM Dong-Kyu LEE Terry A. KLEIN Heung-Chul KIM Won-Ja LEE Heung Ku IM 《Entomological Research》2010,40(4):202-210
After re-emergence of malaria in 1993, a continued increase in Plasmodium vivax cases was observed from 1993 to 2006 in northern Gyeonggi and Gangwon Provinces adjacent to the demilitarized zone separating North from South Korea. Annual parasite incidence per 1000 people ranged from 0.33 in 2004 to 0.89 in 2006. While malaria case rates declined (22.6%) in 2004, they increased 75.1% in 2005 and 51.7% in 2006 from the previous years. An initial incorrect diagnosis of 46.8% of malaria cases as common cold resulted in a mean delay of 1.3 days for the detection malarial parasites. Of the total cases, 10.2% from December to May were due to latent intrinsic incubation infections acquired the previous malaria season and the rest of the cases from June to November were either latent or short incubation infections. Overall, the peak anopheline population occurred from July to September, resulting in a similar peak in malaria cases. While malaria cases increased during 2005–2006, anopheline populations, based on trap indices, were not significantly different during 4 years of surveillance. To decrease the malaria patient infective period to mosquitoes, public health centers in Paju and Cheorwon in 2006 prescribed chloroquine + primaquine at days 0–3 after initial malaria diagnosis followed by an additional 11 days of primaquine (early primaquine treatment), rather than chloroquine on days 0–3 and primaquine on days 4–17 (delayed primaquine treatment). A reduction in the malaria parasite incidence during 2007 was recorded for the two locations offering the early primaquine treatment relative to other locations using the delayed primaquine treatment. 相似文献
94.
Nine novel microsatellite loci were isolated from Oplegnathus fasciatus by screening an enriched genomic library using nonradioactive PCR (polymerase chain reaction) techniques. All loci were found to be polymorphic with an average of 8.1 alleles per locus (range 3–15). The mean observed and expected heterozygosities were 0.71 (range 0.40–1.00) and 0.74 (range 0.50–0.90), respectively. Two loci showed significant Hardy–Weinberg disequilibrium at the P < 0.05 level. The high variabilities revealed in this study suggest that these microsatellite loci should provide useful markers for genetic variation monitoring of O. fasciatus. 相似文献
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96.
Heung Chul KIM Sung Tae CHONG Peter V. NUNN Terry A. KLEIN 《Entomological Research》2010,40(2):136-144
Adult mosquito surveillance was conducted using New Jersey light traps from 1 May to 15 October during 2007 at 14 US Army and three Air Force installations and two US Army training sites located in six provinces in the Republic of Korea to determine threshold levels to initiate pesticide applications based on trap indices. A total of 24 013 adult mosquitoes (19 763 [82.3%] females and 4250 [17.1%] males) comprising 16 species (including five members of the Anopheles Hyrcanus Group) and belonging to seven genera were collected. Females of the most commonly collected species were members of the Anopheles Hyrcanus Group (52.1%), followed by, Culex tritaeniorhynchus Giles (20.6%), Culex pipiens Coquillett (13.8%), and Aedes vexans nipponii (Theobald) (11.6%). Trap indices varied widely for species over their range, due in part, to geographical distribution and degree of association with urban or rural communities. 相似文献
97.
BYEONG G. KIM PETER P. McCANN THOMAS J. BYERS 《The Journal of eukaryotic microbiology》1987,34(3):264-266
Proliferation of Acanthamoeba castellanii (Neff strain) in either a broth medium or a defined medium was arrested by α-monofluoromethyldehydroornithine (Δ-MFMOme), α-difluoromethylornithine (DFMO), and (R, R')-δ-methyl-α-acetylenic putrescine (MAP), three specific inhibitors of ornithine decarboxylase. Although all three inhibited the ameba enzyme, Δ-MFMOme was the most effective inhibitor of multiplication. Growth inhibition was reversed by the addition of polyamines. The inhibitors did not induce differentiation by themselves although DFMO caused encystment when supplemented with CaC12 or MgSO4. 相似文献
98.
New microsatellite DNA markers from brown sole were developed and characterized. Fourteen primer sets were designed from 40 microsatellite regions. Eight of 14 loci exhibited variations comprising 8–31 alleles. Observed and expected heterozygosities ranged from 0.611 to 0.833 and from 0.647 to 0.968 among 36 individuals, respectively. Phz3, Phz8 and Phz12 significantly deviated from Hardy–Weinberg equilibrium, and there was a significant linkage disequilibrium between Phz2 and Phz12. Seven of eight loci conformed to the Mendelian manner of inheritance in a full‐sib family. Seven to four loci of three related species were cross‐amplified by primers for brown sole. 相似文献
99.
In this study, we isolated and characterized nine polymorphic trinucleotide microsatellites (CAG and CCT) from the crucian carp (Carassius auratus). The number of alleles per locus ranged from two to six. Five loci showed a significantly excess homozygosity, and a genetic linkage between CAL0102 and CAL0495 was strongly suggested. Our results confirmed the triploidy of Korean individuals, and the microsatellites were found to be useful for analysing the allelic state of the polyploid crucian carp. 相似文献