Stomatal responses to humidity and temperature in darkness

Stomatal responses to leaf temperature (T_l) and to the mole fractions of water vapour in the ambient air (w_a) and the leaf intercellular air spaces (w_i) were determined in darkness to remove the potential effects of changes in photosynthesis and intercellular CO₂ concentration. Both the steady‐state and kinetic responses of stomatal conductance (g_s) to w_a in darkness were found to be indistinguishable from those in the light. g_s showed a steep response to the difference (Δw) between w_a and w_i when w_a was varied. The response was much less steep when w_i was varied. Although stomatal apertures responded steeply to T_l when Δw was held constant at 17 mmol mol^?1, the response was much less steep when Δw was held constant at about zero. Similar results were obtained in the light for Δw = 15 mmol mol^?1 and Δw ≈ 0 mmol mol^?1. These results are discussed in the context of mechanisms for the stomatal response to humidity.     

Ultrastructure and Vacuolar Movements in the Free-Living Diplomonad Trepomonas agilis Klebs*

SYNOPSIS. The structure of Trepomonas agilis communis Klebs is described from light and electron microscope observations on 2 clone isolates of the organism. The surface membrane shows marked differentiation into an extremely thick (16 nm) symmetric membrane which covers the greater part of the body, and a thinner (∼ 10–12 nm) asymmetric membrane which lines the 2 lateral oral grooves and the posterior channel connecting them; a similar asymmetric membrane covers the flagella. Thorium dioxide staining suggests a denser distribution of acidic carbohydrate groups on the asymmetric membrane. The pathways of cytoplasmic streaming observed in the living flagellate coincide with those of microtubule bands arising close to the flagellar basal bodies and it is suggested that the bands play an orienting role in the streaming of food vacuoles. The contractile vacuole undergoes diastole in the anterior (postnuclear) cytoplasm, and is formed by coalescence of smaller vesicles. At systole the entire vacuole migrates to the posterior extremity to discharge into the posterior channel; the route of exit lacks guiding structural elements. Features of the flagellate's physiology and organization are discussed in relation to the observed lack of mitochondria, microbodies and Golgi apparatus in diplomonads.     

Scale and uncertainty in modeled soil organic carbon stock changes for US croplands using a process‐based model

Process‐based model analyses are often used to estimate changes in soil organic carbon (SOC), particularly at regional to continental scales. However, uncertainties are rarely evaluated, and so it is difficult to determine how much confidence can be placed in the results. Our objective was to quantify uncertainties across multiple scales in a process‐based model analysis, and provide 95% confidence intervals for the estimates. Specifically, we used the Century ecosystem model to estimate changes in SOC stocks for US croplands during the 1990s, addressing uncertainties in model inputs, structure and scaling of results from point locations to regions and the entire country. Overall, SOC stocks increased in US croplands by 14.6 Tg C yr^?1 from 1990 to 1995 and 17.5 Tg C yr^?1 during 1995 to 2000, and uncertainties were ±22% and ±16% for the two time periods, respectively. Uncertainties were inversely related to spatial scale, with median uncertainties at the regional scale estimated at ±118% and ±114% during the early and latter part of 1990s, and even higher at the site scale with estimates at ±739% and ±674% for the time periods, respectively. This relationship appeared to be driven by the amount of the SOC stock change; changes in stocks that exceeded 200 Gg C yr^?1 represented a threshold where uncertainties were always lower than ±100%. Consequently, the amount of uncertainty in estimates derived from process‐based models will partly depend on the level of SOC accumulation or loss. In general, the majority of uncertainty was associated with model structure in this application, and so attaining higher levels of precision in the estimates will largely depend on improving the model algorithms and parameterization, as well as increasing the number of measurement sites used to evaluate the structural uncertainty.     

Viral Properties of Scrapie

ALTHOUGH scrapie agent has many unusual features^1–4, some aspects of its behaviour are analogous to viral properties⁵. The manner in which the agent invades the nervous system after peripheral inoculation immediately recalls such typically neurotropic viruses as poliomyelitis or rabies. After inoculation into the fore-limb changes occur first in the cervical cord; following injection into the leg they appear first in the lumbar cord⁶. We now report another way in which scrapie behaves as a classical virus.     

Population studies of the beech leaf mining weevil (Rhynchaenus fagi) in Ireland and Scotland

Abstract. 1. Population budgets for Rhynchaenus fagi, based for the most part on samples of beech leaves taken in June, were constructed for a 10-year period (Northern Ireland) and a 3-year period (Scottish borders).
2. There were years of sustained population growth and sustained decline, but not enough evidence to suggest a cycle. Similar population levels were found at both sites in all but one year.
3. Key factors were associated with the adult stage, although egg mortality may also make a significant contribution to population change.
4. Unknown sources of mortality embodied in the residual mortality were also suspected of acting in a density dependent manner, compensating for variation in parasitism.
5. Death rate of larvae within mines was related to May temperature in Northern Ireland, but not so clearly in Scotland.     

Historical and Ecological Factors in the Biogeography of Aposematic Neotropical Butterflies

SYNOPSIS. Quantitative analysis of low-level (mostly subspecific)geographic differentiation of aposematic color patterns in threegroups of forest butterflies (Heliconiini, Ithomiinae, and Troidini),in 1,550 half-degree quadrants throughout the Neotropics, indicatesfour principal species-endemicity regions, 44 principal subspecies-endemicitycenters, and 300 quadrants of subspecies hybridization. Theendemism centers are different in outline but nearly identicalin position for each of the three ecologically different groups.Basic data on present and past physical environments (climate,soils, geomorphology, and vegetation structures), when integratedinto maps, show 1) regions of favorable ecological conditionsfor forest organisms in the present, 2) regions of environmentalconformities and rapid transitions in the present, and 3) regionsof high probability for humid forest persistence during themajor landscape changes at the end of the last glacial period,13,000–20,000 yr ago. Species diversity (a local phenomenon)is readily related to favorable modern ecological conditionsand environmental microheterogeneity, promoted by unpredictablemild disturbance. Biogeographical patterns of evolution at thelevel of regional subspecies correlate very well with presumedpaleoecological conditions. The biogeographical data also supportan hypothesis of differentiation resulting from areas of rapidenvironmental change; most of the hybridization quadrants fallwithin transition zones. There is lower correlation betweencenters of endemism and zones of en vironmental conformity,but some individual cases of differentiation in the butterfliesmay be adequately explained by modern ecological factors. Speciespresently undergoing differentiation in isolated habitat islands(Amazonian savannas and high-altitude Andean forests and paramos)may offer the best opportunities for observation of evolutionaryprocesses in the present.     

Reintroducing capercaillie (Tetrao urogallus) into southern Scotland: identification of minimum viable populations at potential release sites

Capercaillie (Tetrao urogallus) populations are declining in western parts of their range, including Scotland. It has been proposed that their numbers, and the extent of their range in Scotland be increased to reduce the risk of a second extinction in the UK. The feasibility of a reintroduction of capercaillie to coniferous plantations in southern Scotland was assessed by undertaking a population viability analysis. Following a review of capercaillie ecology and habitat requirements, VORTEX population simulation software was used to identify a minimum viable population (MVP). From this the minimum dynamic area of suitable habitat required in order to support such a MVP was then calculated. It was estimated that a minimum of 60 individuals would be required in approximately 5000ha of habitat in order for the population to have a >0.95 probability of surviving for 50 years. Supplementation of populations with two unrelated individuals every five years reduced the MVP to ten individuals. Further simulations were run in order to establish the sensitivity of the model to changes in three key parameters. Assessment of areas of suitable habitat identified two potential release sites, Wauchope and Newcastleton forests, in southern Scotland. Some practical considerations relating to management of a release population are outlined. It was concluded that an appropriately planned and resourced reintroduction was feasible.     

Elevated volatile concentrations in high‐nutrient plants: do insect herbivores pay a high price for good food?

1. A tritrophic perspective is fundamental for understanding the drivers of insect–plant interactions. While host plant traits can directly affect insect herbivore performance by either inhibiting or altering the nutritional benefits of consumption, they can also have an indirect effect on herbivores by influencing rates of predation or parasitism. 2. Enhancing soil nutrients available to trees of the genus Eucalyptus consistently modifies plant traits, typically improving the nutritional quality of the foliage for insect herbivores. We hypothesised that resulting increases in volatile essential oils could have an indirect negative effect on eucalypt‐feeding herbivores by providing their natural enemies with stronger host/prey location cues. 3. Eucalyptus tereticornis Smith seedlings were grown under low‐ and high‐nutrient conditions and the consequences for the release of volatile cues from damaged plants were examined. The influence of 1,8‐cineole (the major volatile terpene in many Eucalyptus species) on rates of predation on model caterpillars in the field was then examined. 4. It was found that the emission of cineole increased significantly after damage (artificial or herbivore), but continued only when damage was sustained by herbivore feeding. Importantly, more cineole was emitted from high‐ than low‐nutrient seedlings given an equivalent amount of damage. In the field, predation was significantly greater on model caterpillars baited with cineole than on unbaited models. 5. These findings are consistent with the hypothesis that any performance benefits insect herbivores derive from feeding on high‐nutrient eucalypt foliage could be at least partially offset by an increased risk of predation or parasitism via increased emission of attractive volatiles.