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51.
Ammonium tolerance in the cyanobacterium Synechocystis sp. strain PCC 6803 and the role of the psbA multigene family 总被引:2,自引:0,他引:2
Ammonium is one of the major nutrients for plants, and a ubiquitous intermediate in plant metabolism, but it is also known to be toxic to many organisms, in particular to plants and oxygenic photosynthetic microorganisms. Although previous studies revealed a link between ammonium toxicity and photodamage in cyanobacteria under in vivo conditions, ammonium‐induced photodamage of photosystem II (PSII) has not yet been investigated with isolated thylakoid membranes. We show here that ammonium directly accelerated photodamage of PSII in Synechocystis sp. strain PCC6803, rather than affecting the repair of photodamaged PSII. Using isolated thylakoid membranes, it could be demonstrated that ammonium‐induced photodamage of PSII primarily occurred at the oxygen evolution complex, which has a known binding site for ammonium. Wild‐type Synechocystis PCC6803 cells can tolerate relatively high concentrations of ammonium because of efficient PSII repair. Ammonium tolerance requires all three psbA genes since mutants of any of the three single psbA genes are more sensitive to ammonium than wild‐type cells. Even the poorly expressed psbA1 gene, whose expression was studied in some detail, plays a detectable role in ammonium tolerance. 相似文献
52.
The transpiration of Tropaeolum plants, grown in water culture,on full nutrient solutions and on solutions deficient in oneof the following, i.e. boron, copper, manganese, and zinc, wasstudied. The transpiration rate of the control plants grownon the full nutrient solution was significantly higher thanthat of the deficient ones. The more acute the deficiency, thegreater was the disparity. Under the condition of the experiment, the control plants weresmaller in size, having fewer and smaller leaves than the deficientones, but the foliage was much greener and healthier in appearance. 相似文献
53.
Identifying factors that influence the survival of individuals during disturbance is critical to understanding patterns of species reassembly within ecological communities. Although most studies of recovery of populations post‐burning acknowledge the potentially important contribution of animals surviving in situ, few have measured the effectiveness of refugia. This paper tests the hypothesis that some plants with tightly packed leaf‐bases provide a refuge for invertebrates during fire (even when the plants themselves burn) by using the highly flammable grass tree (Xanthorrhoeaceae: Xanthorrhoea). Invertebrates were sampled from four unburnt and five experimentally burnt grass trees (Xanthorrhoea preissii Endl.). Also collected were invertebrates fleeing during burning. The dataset comprises 949 specimens, representing 81 species from 18 orders, of which 749 individuals were from unburned plants. Slaters (Isopoda), silverfish (Thysanura), spiders (Araneae) and bugs (Hemiptera) dominated assemblages of the unburnt grass trees. Despite grass trees burning at temperatures of up to 515°C, some invertebrates survived in situ. Species‐specific microhabitat preferences within the plant appeared to influence survivorship. Species collected in the crown of unburned plants were found more often alive on burnt plants than species typically inhabiting the dead skirt of decaying leaves (thatch). We contend that the mechanism causing differential mortality is fire temperature. In the dead skirt, temperatures reached 225.33 ± 66.57°C. In contrast, a region of mild temperature (25.00 ± 3.54°C) persisted throughout burning near the apical meristem (within the crown). We conclude that grass trees are a potential reservoir from which invertebrates might re‐colonize recently burnt areas. However, owing to species‐specific microhabitat preferences and differential mortality across microhabitats, the invertebrate assemblage remaining in situ will be restricted taxonomically compared with the original grass tree fauna. Moreover, different fire regimes might mediate the effectiveness of grass trees as refugia. Finally, we argue that in situ survival of invertebrates within plants with tightly packed leaf‐bases is an unrecognized global phenomenon applicable to a wide array of plant taxa. 相似文献
54.
55.
Eight microsatellite loci were isolated and characterized from the Florida crown conch (Melongena spp.). Approximately 500 individuals from 20 populations encompassing all known Melongena spp. groups in Florida and Alabama were genotyped at all loci. High levels of variation were found with eight to 28 alleles per locus and observed per locus heterozygosity ranging from 0.148 to 0.779 (mean 0.624). One locus, Mco5, was monomorphic in more than half of the populations. These loci can provide useful information for a variety of studies ranging from parentage to population‐level analyses of the Florida crown conch. 相似文献
56.
KARL J. AUFDERHEIDE 《The Journal of eukaryotic microbiology》1978,25(3):362-365
SYNOPSIS. Following electroshock-induced extrusion of its inserted trichocysts, Paramecium tetraurelia rapidly begins replacement of the population of lost organelles. Light microscopy of the cortical insertion of new trichocysts reveals a series of characteristic motility activities. An uninserted trichocyst in the cyclotic flow of the cell appears to be “captured” and removed to the noncyclotic, subcortical regions. The trichocyst then makes a series of saltatory motions which apparently serve to transport it to the cortex, with proper orientation (tip first) for insertion. Trichocyst saltations end with either cortical insertion of the organelle, or return to cyclosis. If the trichocyst is inserted, it makes a series of unique pivoting movements around the motionless tip. This form of motility, termed “wobble,” continues for a short period of time. After cessation of wobble, the insertion of the trichocyst is apparently complete, since no further motility is observed. With the aid of these observations it was possible to identify saltatory motility as the means for transporting trichocysts to the cortex for insertion, and also to observe a motility of unknown significance (wobble) apparently associated with the process of cortical insertion. 相似文献
57.
Monoclonal antibodies (MAb) to tumor-associated antigens are attracting much attention for tumor therapy. Melanomas belong to the tumors most studied in this respect, and several melanoma-associated antigens have been studied in great detail. These include the melanoma-associated glycoprotein p97, the melanoma-associated proteoglycan, and glycolipid antigens. Although none of the antigens is absolutely specific for tumor, the degree of relative specificity appears to be sufficient to use several of the melanoma antigens as therapeutic “targets”. Antimelanoma MAb can be applied therapeutically in several ways. The most straightforward approach is use of MAb without further modification. MAb which kill melanoma cells in the presence of human serum as the source of complement or mediate antibody-dependent cellular cytotoxicity with human natural killer (NK) cells or macrophages as effectors are logical choices for this. Some cases of partial or even complete regression of metastatic melanoma have been observed in patients treated with such MAb. Combinations of such MAb with interleukin 2 (IL-2) or other immunological response modifiers are of great interest. Alternatively, one may use antimelanoma MAb (or fragments prepared from MAb) as carriers of antitumor agents, including radioactive isotopes, toxins, or chemotherapeutic drugs. Although it is premature to make any conclusions about the efficacy of such conjugates, we are optimistic that it will be feasible by using the right combination of MAb and antitumor agent to achieve therapeutic benefit. Another approach is to develop therapeutic “vaccines” for active immunization, once an antigen characterized by using a MAb has proven to have a relatively high level of tumor selectively. Anti-idiotypic antibodies and live recombinant viruses inducing tumor antigen expression in infected cells provide alternative strategies to this approach. 相似文献
58.
KARL KRAL 《Physiological Entomology》2013,38(1):1-12
Mantispids (Neuroptera: Mantispidae) are remarkable insects as a result of their close resemblance to the praying mantis (order Mantodea). Although not closely related phylogenetically, as a result of similar selective pressures, both mantispids and mantids have evolved powerful raptorial forelegs for capturing insects. Another striking feature is the hypermetamorphosis in mantispid development, as well as the parasitizing behaviour of the first‐instar larvae. The present review focuses on the role of mantispid vision. First, the morphology and functional significance of the larval eyes (stemmata) are examined. In principle, the stemmata are suitable for spatial vision because of their arrangement and structure. This is then followed by a discussion of how adult mantispids are able to capture fast‐moving insects successfully, although, in contrast to the praying mantis, mantispids rely on superposition eyes rather than on apposition eyes with a frontal region of high acuity. For both larvae and adults, comparisons are made with other insect groups. The present review also addresses the role of mantispid vision as an important cue for triggering mating behaviour; accordingly, sex‐specific differences are considered. Finally, vision in the context of orientation flight is discussed. 相似文献
59.
ASTRID N. SCHWALB KARL COTTENIE MARK S. POOS JOSEF D. ACKERMAN 《Freshwater Biology》2011,56(8):1509-1518
1. Freshwater unionid mussels are a highly imperilled group. Their dispersal abilities depend on the availability and the movement of host fish on which their parasitic mussel larvae develop. 2. We examined the relationship between the dispersal abilities of unionid mussels and their conservation status on a regional (SW Ontario) scale and their distribution and abundance on a catchment scale (Sydenham River, SW Ontario) by determining host specificity and estimating the dispersal abilities of mussels on fish from a review of the literature. 3. On the regional scale, we found that mussels with the most precarious conservation status relied on host fish with short movement distances, whereas vulnerable and more secure mussel species had host fish with 2–3 orders of magnitude larger movement distances. We were not able to detect a clear pattern on the catchment scale. 4. Our results suggest that limited dispersal by host fish affects the abundance and distribution of unionid mussels and ultimately their conservation status on a regional scale. Information on dispersal limitations because of differences in host fish communities should be included in conservation and management decisions to ensure connectivity and maintain functioning mussel metacommunities. 相似文献
60.
PIETER VANORMELINGEN KARL COTTENIE ERIK MICHELS KOENRAAD MUYLAERT WIM VYVERMAN LUC DE MEESTER 《Freshwater Biology》2008,53(11):2170-2183
1. The recognition that both local and regional processes act together in shaping local communities makes determining their relative roles in natural communities central to understanding patterns in community structure. 2. We investigated the relative influence of these processes on the phytoplankton communities of a highly interconnected pond system. We sampled the phytoplankton communities of 28 ponds concurrently with 20 local environmental variables. 3. We found that phytoplankton community variation, in terms of both phytoplankton community composition (PCC) and diversity, was only significantly explained by local environmental variables. These were mainly associated with the contrasting clear‐water and turbid ecological states of the shallow ponds studied. Clear‐water conditions favoured only a few taxa, resulting in a significantly lower taxon diversity and richness under these conditions. 4. The failure to explain variation in PCC by a dispersal model based on the water flow between ponds points at very effective species sorting. This is attributed to the high population turn‐over rates and sensitivity to environmental conditions of phytoplankton communities. Some evidence was found, however, that dispersal influences local communities through mass effects between neighbouring ponds. 5. Overall, our results emphasize both the strong selection pressure that components of the food web exert on phytoplankton communities and the high potential of these communities to respond to such environmental change, thereby effectively opposing the homogenizing effects of continuous dispersal. 相似文献