全文获取类型
收费全文 | 5884篇 |
免费 | 579篇 |
国内免费 | 1464篇 |
出版年
2024年 | 17篇 |
2023年 | 100篇 |
2022年 | 205篇 |
2021年 | 294篇 |
2020年 | 230篇 |
2019年 | 293篇 |
2018年 | 243篇 |
2017年 | 178篇 |
2016年 | 269篇 |
2015年 | 355篇 |
2014年 | 459篇 |
2013年 | 468篇 |
2012年 | 598篇 |
2011年 | 605篇 |
2010年 | 391篇 |
2009年 | 360篇 |
2008年 | 430篇 |
2007年 | 388篇 |
2006年 | 362篇 |
2005年 | 272篇 |
2004年 | 236篇 |
2003年 | 236篇 |
2002年 | 218篇 |
2001年 | 121篇 |
2000年 | 112篇 |
1999年 | 95篇 |
1998年 | 69篇 |
1997年 | 53篇 |
1996年 | 47篇 |
1995年 | 23篇 |
1994年 | 33篇 |
1993年 | 17篇 |
1992年 | 22篇 |
1991年 | 14篇 |
1990年 | 13篇 |
1989年 | 9篇 |
1988年 | 8篇 |
1987年 | 16篇 |
1986年 | 7篇 |
1985年 | 4篇 |
1984年 | 6篇 |
1983年 | 3篇 |
1982年 | 5篇 |
1980年 | 7篇 |
1978年 | 4篇 |
1974年 | 5篇 |
1971年 | 4篇 |
1967年 | 2篇 |
1966年 | 2篇 |
1950年 | 2篇 |
排序方式: 共有7927条查询结果,搜索用时 15 毫秒
31.
云南普通马矮型马蛋白多态性及其品种分化关系 总被引:3,自引:1,他引:2
本文运用蛋白电泳技术对来自云南省文山州马关县和麻栗坡县的21匹普通马和14匹矮型马进行了分析。共分析遗传座位44个,其中有10个座位检测到多态性。根据分子钟假说和相应的公式,推算两者的分歧时间约为18.5万年。 相似文献
32.
发情期小灵猫行为的研究 总被引:9,自引:0,他引:9
本文对笼养小灵猫发情期的繁殖行为进行了观察。结果表明:小灵猫一年有两次发情期,绝大部分的小灵猫在春季(2-4月)发情,少数个体在秋季(8月底至9月)出现第2次发情。发情期雄性小灵猫频繁地发出求偶叫声和增加擦香次数。雄猫的擦香行为和求偶叫声可促使雌猫的发情,发情期一系列性行为对雌雄交配的成功与否有重要的意义。 相似文献
33.
The effects of 24 hr light-dark cycles on the circadian conidiation rhythm inNeurospora crassa were compared among will-typefrq
+ and clock mutantsfrq
+,frq
3,frq
7,frq
9 andfrq
11. The minimum length of the light period necessary for complete entrainment to the light-dark cycles was almost 2 hr infrq
+,frq
3 andfrq
7 strains. The minimum duration of the dark period necessary for the appearance of circadian conidiation was almost 4 hr in
all of the strains except thefrq
11 strain. The phase of the conidiation rhythm was dependent on the light to dark transition in thefrq
1 strain in all light-dark cycles examined and in thefrq
+ andfrq
3 strains when the light period was shorter than 16 hr. In contrast, the phase of thefrq
7 strain was dependent on the light to dark transition when the light period was shorter than 10 hr. 相似文献
34.
35.
(p-Amidinophenyl)methanesulfonyl fluoride, an irreversible inhibitor of serine proteases 总被引:4,自引:0,他引:4
p-(Amidinophenyl)methanesulfonyl fluoride (p-APMSF) has been synthesized and shown to be a specific, irreversible inhibitor of the class of plasma serine proteases which demonstrate substrate specificity for the positively charged side chains of the amino acid lysine or arginine. In equimolar concentration, this compound causes immediate and complete irreversible inhibition of bovine trypsin and human thrombin. A 5-10-fold molar excess of reagent over enzyme is required to achieve complete irreversible inhibition of bovine Factor Xa, human plasmin, human C1-r, and human C1-s. the Ki of p-APMSF for all of the above-mentioned proteases is between 1 and 2 microM. In contrast, p-APMSF in large molar excess does not inactivate chymotrypsin or acetylcholinesterase. The unique reactivity of p-APMSF has been further shown in comparison with the related compound p-nitrophenyl (p-amidinophenyl)methanesulfonate which is an active-site titrant for thrombin but reacts poorly with Factor Xa, C1-r, and C1-s and is not hydrolyzed by bovine trypsin or human plasmin. Similarly, (p-amidinophenyl)methanesulfonate has a Ki of 30 microM for thrombin but is a poor inhibitor of trypsin, Factor Xa, C1-r, C1-s, and plasmin. Studies with bovine trypsin have demonstrated that the inhibitory activity of p-APMSF is the result of its interaction with the diisopropyl fluorophosphate reactive site. The unique reactivity of this inhibitor classifies it as one of the most effective active site directed reagents for this class of serine proteases. Collectively, these results suggest that the primary substrate binding site of these enzymes, which share a high degree of structural homology, do in fact significantly differ from each other in their ability to interact with low molecular weight inhibitors and synthetic substrates. 相似文献
36.
Leucocyte sodium efflux rate constants and intracellular electrolyte contents were estimated in 13 patients with untreated essential hypertension. There was no correlation between intracellular sodium or potassium content or efflux rate constant and blood pressure. The patients were then treated with oral nifedipine and blood pressure controlled. Sodium efflux rate constants and electrolyte contents were estimated one and three months after the start of treatment. There was a significant fall in blood pressure, but mean sodium efflux rate constant and intracellular sodium content were unchanged. There was no correlation between the fall in blood pressure, initial sodium efflux, or intracellular sodium content. These data do not support the hypothesis that the sodium pump and intracellular sodium content have a direct role in generating raised blood pressure, or that treatment of hypertension with calcium antagonists corrects a fundamental alteration of calcium-sodium exchange across the cell membrane. 相似文献
37.
38.
A new simplified procedure for determination of nitric oxide (NO) in biological solutions is described utilizing a new reducing system of nitric oxide prior to chemiluminescence. Advantages of the new method makes heating of the reducing solution unnecessary and avoids cooling and condensation of generated vapors. Only traces of acid with a high boiling point are used. The method permits analysis of small sample volumes (200 microL). The basal production of nitric oxide by freshly harvested endothelial cells ranged from 100 to 880 picomoles. 相似文献
39.
The entomopathogenic fungus,Beauveria bassiana (Balsamo) Vuillemin, was applied to whorl-stage (V7) corn,Zea mays L., by foliar application of a granular formulation of corn grits containing conidia or by injection of a conidial suspension.
All plants were infested with European corn borer larvae,Ostrinia nubilalis (Hübner), at the V7 (whorl), V12 (late-whorl), or V17 (pretassel) stage of plant development. Plants infested at whorl and
late-whorl stages had significantly more European corn borer tunneling than did plants infested at the pretassel stage. The
percentage of plants colonized byB. bassiana did not differ significantly among the whorl, late-whorl, and pretassel stages. As the plants matured,B. bassiana was isolated from different plant areas, with the pith more frequently colonized than the leaf collars. Foliar application
ofB. bassiana provided immediate suppression ofO. nubilalis in those plants infested at whorl stage. The reduced efficacy ofB. bassiana at the intermediate plant stages relative to efficacy at harvest is discussed.
The mention of firm names or trade products does not imply that they are endorsed or recommended by the U.S. Department of
Agriculture over other firms or similar products not mentioned. 相似文献
40.